LEU1_RHOPA
ID LEU1_RHOPA Reviewed; 524 AA.
AC Q6N858;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=2-isopropylmalate synthase {ECO:0000255|HAMAP-Rule:MF_01025};
DE EC=2.3.3.13 {ECO:0000255|HAMAP-Rule:MF_01025};
DE AltName: Full=Alpha-IPM synthase {ECO:0000255|HAMAP-Rule:MF_01025};
DE AltName: Full=Alpha-isopropylmalate synthase {ECO:0000255|HAMAP-Rule:MF_01025};
GN Name=leuA {ECO:0000255|HAMAP-Rule:MF_01025}; OrderedLocusNames=RPA2046;
OS Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Rhodopseudomonas.
OX NCBI_TaxID=258594;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-98 / CGA009;
RX PubMed=14704707; DOI=10.1038/nbt923;
RA Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L.,
RA Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R.,
RA Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C.,
RA Harrison F.H., Gibson J., Harwood C.S.;
RT "Complete genome sequence of the metabolically versatile photosynthetic
RT bacterium Rhodopseudomonas palustris.";
RL Nat. Biotechnol. 22:55-61(2004).
CC -!- FUNCTION: Catalyzes the condensation of the acetyl group of acetyl-CoA
CC with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-
CC hydroxy-4-methylpentanoate (2-isopropylmalate). {ECO:0000255|HAMAP-
CC Rule:MF_01025}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-
CC isopropylmalate + CoA + H(+); Xref=Rhea:RHEA:21524, ChEBI:CHEBI:1178,
CC ChEBI:CHEBI:11851, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.13;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01025};
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 1/4. {ECO:0000255|HAMAP-
CC Rule:MF_01025}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01025}.
CC -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC family. LeuA type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_01025}.
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DR EMBL; BX572599; CAE27487.1; -; Genomic_DNA.
DR RefSeq; WP_011157601.1; NC_005296.1.
DR AlphaFoldDB; Q6N858; -.
DR SMR; Q6N858; -.
DR STRING; 258594.RPA2046; -.
DR PRIDE; Q6N858; -.
DR EnsemblBacteria; CAE27487; CAE27487; RPA2046.
DR GeneID; 66893091; -.
DR KEGG; rpa:RPA2046; -.
DR eggNOG; COG0119; Bacteria.
DR HOGENOM; CLU_022158_0_1_5; -.
DR OMA; NTMRMLV; -.
DR PhylomeDB; Q6N858; -.
DR BioCyc; RPAL258594:TX73_RS10425-MON; -.
DR UniPathway; UPA00048; UER00070.
DR Proteomes; UP000001426; Chromosome.
DR GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.20.20.70; -; 1.
DR Gene3D; 3.30.160.270; -; 1.
DR HAMAP; MF_01025; LeuA_type1; 1.
DR InterPro; IPR013709; 2-isopropylmalate_synth_dimer.
DR InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR036230; LeuA_allosteric_dom_sf.
DR InterPro; IPR005671; LeuA_bact_synth.
DR InterPro; IPR000891; PYR_CT.
DR Pfam; PF00682; HMGL-like; 1.
DR Pfam; PF08502; LeuA_dimer; 1.
DR SMART; SM00917; LeuA_dimer; 1.
DR SUPFAM; SSF110921; SSF110921; 1.
DR TIGRFAMs; TIGR00973; leuA_bact; 1.
DR PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR PROSITE; PS50991; PYR_CT; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW Leucine biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..524
FT /note="2-isopropylmalate synthase"
FT /id="PRO_1000149258"
FT DOMAIN 12..274
FT /note="Pyruvate carboxyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01151"
SQ SEQUENCE 524 AA; 57551 MW; EF9D39C1079DD49B CRC64;
MTTTTQSEQD RVIIFDTTLR DGEQCPGATM TFEEKLNVAR MLDDMGVDVI EAGYPFASDG
DFEAVHEIAK RSKNSVICGL SRAAHKDIDR CAEAIKPAER GRIHTFLSTS PVHMKYKLQM
EAAQVYEMVI SSVTRARNHT DDVEWSAEDA TRTEFDFLCR CIEAAIKAGA TTINLPDTVG
YAVPEEYREL FRKVRETVPN SDKARFSVHC HNDLGMAVAN SMAGVAGGAR QIECTINGIG
ERAGNAALEE VVMAMRVRQD RLPYWNRIET TMLTHASKTV SAATSFPVQY NKAIVGRNAF
AHESGIHQDG MIKNAQTYEI MTPETVGVKG TSLVMGKHSG RAGLIHKMEE LGYKLSRNQI
EDVFVRFKAL ADRKKDVYDE DIEALVDEQL LHGQDQIKLM SLTVIAGTHG PQRATMKLDV
DGQIRIEEAE GNGPVDAVFN CIKALVPHDA KLELYQVHAV TEGTDAQAEV SVRLSHEGRS
MTARAADPDT LVASAKAYLG ALNKIVAKRQ RSVREDAPAV AVAG