LEU1_RHORT
ID LEU1_RHORT Reviewed; 558 AA.
AC Q2RT17;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=2-isopropylmalate synthase {ECO:0000255|HAMAP-Rule:MF_00572};
DE EC=2.3.3.13 {ECO:0000255|HAMAP-Rule:MF_00572};
DE AltName: Full=Alpha-IPM synthase {ECO:0000255|HAMAP-Rule:MF_00572};
DE AltName: Full=Alpha-isopropylmalate synthase {ECO:0000255|HAMAP-Rule:MF_00572};
GN Name=leuA {ECO:0000255|HAMAP-Rule:MF_00572}; OrderedLocusNames=Rru_A1928;
OS Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 /
OS NCIMB 8255 / S1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Rhodospirillum.
OX NCBI_TaxID=269796;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1;
RX PubMed=21886856; DOI=10.4056/sigs.1804360;
RA Munk A.C., Copeland A., Lucas S., Lapidus A., Del Rio T.G., Barry K.,
RA Detter J.C., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D.,
RA Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Kyrpides N.C.,
RA Mavromatis K., Richardson P., Rohde M., Goeker M., Klenk H.P., Zhang Y.,
RA Roberts G.P., Reslewic S., Schwartz D.C.;
RT "Complete genome sequence of Rhodospirillum rubrum type strain (S1).";
RL Stand. Genomic Sci. 4:293-302(2011).
CC -!- FUNCTION: Catalyzes the condensation of the acetyl group of acetyl-CoA
CC with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-
CC hydroxy-4-methylpentanoate (2-isopropylmalate). {ECO:0000255|HAMAP-
CC Rule:MF_00572}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-
CC isopropylmalate + CoA + H(+); Xref=Rhea:RHEA:21524, ChEBI:CHEBI:1178,
CC ChEBI:CHEBI:11851, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.13;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00572};
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 1/4. {ECO:0000255|HAMAP-
CC Rule:MF_00572}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00572}.
CC -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC family. LeuA type 2 subfamily. {ECO:0000255|HAMAP-Rule:MF_00572}.
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DR EMBL; CP000230; ABC22728.1; -; Genomic_DNA.
DR RefSeq; WP_011389681.1; NC_007643.1.
DR RefSeq; YP_427015.1; NC_007643.1.
DR AlphaFoldDB; Q2RT17; -.
DR SMR; Q2RT17; -.
DR STRING; 269796.Rru_A1928; -.
DR EnsemblBacteria; ABC22728; ABC22728; Rru_A1928.
DR KEGG; rru:Rru_A1928; -.
DR PATRIC; fig|269796.9.peg.2011; -.
DR eggNOG; COG0119; Bacteria.
DR HOGENOM; CLU_004588_3_0_5; -.
DR OMA; WPDKVID; -.
DR OrthoDB; 840579at2; -.
DR PhylomeDB; Q2RT17; -.
DR UniPathway; UPA00048; UER00070.
DR Proteomes; UP000001929; Chromosome.
DR GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd07942; DRE_TIM_LeuA; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR Gene3D; 3.30.160.270; -; 1.
DR HAMAP; MF_00572; LeuA_type2; 1.
DR InterPro; IPR013709; 2-isopropylmalate_synth_dimer.
DR InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR005668; IPM_Synthase.
DR InterPro; IPR036230; LeuA_allosteric_dom_sf.
DR InterPro; IPR039371; LeuA_N_DRE-TIM.
DR InterPro; IPR000891; PYR_CT.
DR Pfam; PF00682; HMGL-like; 1.
DR Pfam; PF08502; LeuA_dimer; 1.
DR SMART; SM00917; LeuA_dimer; 1.
DR SUPFAM; SSF110921; SSF110921; 1.
DR TIGRFAMs; TIGR00970; leuA_yeast; 1.
DR PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR PROSITE; PS50991; PYR_CT; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW Leucine biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..558
FT /note="2-isopropylmalate synthase"
FT /id="PRO_1000129512"
FT DOMAIN 31..306
FT /note="Pyruvate carboxyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01151"
SQ SEQUENCE 558 AA; 61146 MW; F535CB6CD0611179 CRC64;
MMIDPSKKYR PFPPVALADR TWPSATITKA PIWCAVDLRD GNQALVEPMG ADRKVRMFQL
LCELGYKEIE VGFPSASQTD FDFLRQLIER DMIPADVTIQ VLTQAREDLI SRTFQALEGA
SSAIVHLYNS TSTLQRRVVF ALDRAGITDL AVRGAEMVRE GAAKASASTK LRFQYSPESF
TGTELDYAVE ICEAVMGVFE PTAEAPLILN LPSTVEMATP NVYADQIEWF CRALPHRDRV
LISLHPHNDR GTAVAAAELA LMAGADRIEG TLFGNGERTG NVDLVTLGMN MFTQGIDPGI
DFSNIDHIRE IAEECNRLPV HPRHPYAGDL VFTAFSGSHQ DAINKGLHAM EKTNQDVWEV
PYLPIDPKDI GRSYEAVIRV NSQSGKGGVA HILERDYGIR MPRGLQVEFS KVVQAITDTT
GEEITSRGLW KTFEETYLGA GSPYSFLEHH TRPDPAGKDQ RILTATVKAG GKVRDIDGRG
TGPIEALVDA LRKDSGLAIT IEDYEEHTLR PGSDAQAVAF IKASLPDGRA HYGVGIDANF
VVASLKAVLC AANHLSDK