ID   ARFT_ARATH              Reviewed;         590 AA.
AC   Q9C7I9; Q5IRX2;
DT   26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 3.
DT   25-MAY-2022, entry version 131.
DE   RecName: Full=Auxin response factor 20;
GN   Name=ARF20; OrderedLocusNames=At1g35240; ORFNames=T9I1.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=15659631; DOI=10.1105/tpc.104.028316;
RA   Okushima Y., Overvoorde P.J., Arima K., Alonso J.M., Chan A., Chang C.,
RA   Ecker J.R., Hughes B., Lui A., Nguyen D., Onodera C., Quach H., Smith A.,
RA   Yu G., Theologis A.;
RT   "Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family
RT   members in Arabidopsis thaliana: unique and overlapping functions of ARF7
RT   and ARF19.";
RL   Plant Cell 17:444-463(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX   PubMed=12036261; DOI=10.1023/a:1015207114117;
RA   Hagen G., Guilfoyle T.J.;
RT   "Auxin-responsive gene expression: genes, promoters and regulatory
RT   factors.";
RL   Plant Mol. Biol. 49:373-385(2002).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=18986826; DOI=10.1016/j.tplants.2008.09.006;
RA   Swaminathan K., Peterson K., Jack T.;
RT   "The plant B3 superfamily.";
RL   Trends Plant Sci. 13:647-655(2008).
CC   -!- FUNCTION: Auxin response factors (ARFs) are transcriptional factors
CC       that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the
CC       auxin-responsive promoter elements (AuxREs). Could act as
CC       transcriptional activator or repressor. Formation of heterodimers with
CC       Aux/IAA proteins may alter their ability to modulate early auxin
CC       response genes expression. {ECO:0000269|PubMed:12036261}.
CC   -!- SUBUNIT: Homodimers and heterodimers. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DOMAIN: Interactions between auxin response factors (ARFs) and Aux/IAA
CC       proteins occur through their C-terminal dimerization domains III and
CC       IV.
CC   -!- SIMILARITY: Belongs to the ARF family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG51458.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AY669795; AAT67079.1; -; mRNA.
DR   EMBL; AC069160; AAG51458.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; D86473; D86473.
DR   AlphaFoldDB; Q9C7I9; -.
DR   SMR; Q9C7I9; -.
DR   BioGRID; 25645; 12.
DR   IntAct; Q9C7I9; 10.
DR   STRING; 3702.AT1G35240.1; -.
DR   PaxDb; Q9C7I9; -.
DR   PRIDE; Q9C7I9; -.
DR   ProteomicsDB; 246492; -.
DR   Araport; AT1G35240; -.
DR   TAIR; locus:2206717; AT1G35240.
DR   eggNOG; ENOG502QTME; Eukaryota.
DR   HOGENOM; CLU_002626_4_4_1; -.
DR   PhylomeDB; Q9C7I9; -.
DR   PRO; PR:Q9C7I9; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9C7I9; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR   CDD; cd10017; B3_DNA; 1.
DR   Gene3D; 2.40.330.10; -; 1.
DR   InterPro; IPR010525; ARF_dom.
DR   InterPro; IPR044835; ARF_plant.
DR   InterPro; IPR033389; AUX/IAA_dom.
DR   InterPro; IPR003340; B3_DNA-bd.
DR   InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR   InterPro; IPR000270; PB1_dom.
DR   PANTHER; PTHR31384; PTHR31384; 1.
DR   Pfam; PF02309; AUX_IAA; 1.
DR   Pfam; PF06507; Auxin_resp; 1.
DR   Pfam; PF02362; B3; 1.
DR   SMART; SM01019; B3; 1.
DR   SUPFAM; SSF101936; SSF101936; 1.
DR   PROSITE; PS50863; B3; 1.
DR   PROSITE; PS51745; PB1; 1.
PE   2: Evidence at transcript level;
KW   Auxin signaling pathway; DNA-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..590
FT                   /note="Auxin response factor 20"
FT                   /id="PRO_0000111524"
FT   DOMAIN          495..576
FT                   /note="PB1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT   DNA_BIND        126..224
FT                   /note="TF-B3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00326"
SQ   SEQUENCE   590 AA;  66970 MW;  8332DD4BA48CA628 CRC64;
     METGNVVNAQ PELSGIIDGS KSYMYEQLWK LCAGPLCDIP KLGENVYYFP QGNIELVDAS
     TREELNELQP ICDLPSKLQC RVIAIHLKVE NNSDETYAEI TLMPDTTQVV IPTQSENQFR
     PLVNSFTKVL TASDTSAYGG FFVPKKHAIE CLPPLPLPAQ ELLAKDLHGN QWRFRHSYRG
     TPQRHSLTTG WNEFTTSKKL VKGDVIVFVR GETGELRVGI RRARHQQGNI PSSIVSIDCM
     RHGVIASAKH ALDNQCIFIV VYKPSIRSSQ FIVSYDKFLD AMNNKFIVGS RFTMRFEGDD
     FSERRYFGTI IGVNDFSPHW KCSEWRSLEV QWDEFASFSR PNKVSPWEIE HLMSALNVPR
     SSLLKNKRLR EVNEFGQEIG QLSVASPMNT SLRYRDTTED AMNPSRLLMS YPVQPMPKLN
     YNNQMVTQIE ENITTKAVTN FRLFGVSLAI PLVIKDPIEE IGSDISKLTE GKKFGQSQTL
     RSPIEIQSKQ FGSTRTCTKV QMQGVTIGRA VDLSVLNGYD QLILELEKLF DLKGQLQTRN
     QWKIAFTDSD GYEMLVGDDP WPEFCKMVKK ILIYSKEEVK NLKSSKSLSS