ID   ARF_ENCCU               Reviewed;         207 AA.
AC   Q8SQH8;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=ADP-ribosylation factor;
GN   Name=ARF-1; OrderedLocusNames=ECU08_1930;
GN   and
GN   Name=ARF-2; OrderedLocusNames=ECU10_0090;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL
RP   STAGE, AND SUBCELLULAR LOCATION.
RX   PubMed=16691553; DOI=10.1002/pmic.200500796;
RA   Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.;
RT   "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi
RT   (microsporidia): a reference map for proteins expressed in late sporogonial
RT   stages.";
RL   Proteomics 6:3625-3635(2006).
CC   -!- FUNCTION: GTP-binding protein involved in protein trafficking; may
CC       modulate vesicle budding and uncoating within the Golgi apparatus.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in late sporogonial stages.
CC       {ECO:0000269|PubMed:16691553}.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC       {ECO:0000305}.
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DR   EMBL; AL590448; CAD26496.1; -; Genomic_DNA.
DR   EMBL; AL590449; CAD25729.1; -; Genomic_DNA.
DR   RefSeq; NP_586125.1; NM_001041958.1.
DR   RefSeq; NP_597320.1; NM_001041929.1.
DR   AlphaFoldDB; Q8SQH8; -.
DR   SMR; Q8SQH8; -.
DR   STRING; 284813.Q8SQH8; -.
DR   GeneID; 859742; -.
DR   GeneID; 859771; -.
DR   KEGG; ecu:ECU08_1930; -.
DR   KEGG; ecu:ECU10_0090; -.
DR   VEuPathDB; MicrosporidiaDB:ECU08_1930; -.
DR   VEuPathDB; MicrosporidiaDB:ECU10_0090; -.
DR   HOGENOM; CLU_040729_12_4_1; -.
DR   InParanoid; Q8SQH8; -.
DR   OMA; LWRILNI; -.
DR   OrthoDB; 1271528at2759; -.
DR   Proteomes; UP000000819; Chromosome VIII.
DR   Proteomes; UP000000819; Chromosome X.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR024156; Small_GTPase_ARF.
DR   InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR   PANTHER; PTHR11711; PTHR11711; 1.
DR   Pfam; PF00025; Arf; 1.
DR   PRINTS; PR00328; SAR1GTPBP.
DR   SMART; SM00178; SAR; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51417; ARF; 1.
PE   1: Evidence at protein level;
KW   ER-Golgi transport; Golgi apparatus; GTP-binding; Lipoprotein; Myristate;
KW   Nucleotide-binding; Protein transport; Reference proteome; Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..207
FT                   /note="ADP-ribosylation factor"
FT                   /id="PRO_0000207414"
FT   BINDING         32..39
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         75..79
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         133..136
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   LIPID           2
FT                   /note="N-myristoyl glycine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   207 AA;  23473 MW;  EBFC49FE3A899E75 CRC64;
     MGNMMSKVNN LLYTKLRGLF SGQSERSITM IGLDGAGKTT LLLYLQTGEV HQTVPTLGFN
     CENVTLGSMK FQVWDIGGQN SFMRFWHQYI NEGCGIIYMV DCADPQRFGK SGEELWRILN
     ILNSPRPLLV LANKIDLIRE HERSEVVKSI RNEFNLERYN GPSQVVPISV LQAGSMTSAN
     DENGREIIDA FRWLNKELEK MPRAEAL