ID   ARF_GIAIN               Reviewed;         191 AA.
AC   P26991;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=ADP-ribosylation factor;
OS   Giardia intestinalis (Giardia lamblia).
OC   Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX   NCBI_TaxID=5741;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1577802; DOI=10.1016/s0021-9258(19)50140-5;
RA   Murtagh J.J. Jr., Mowatt M.W., Lee C.-M., Lee F.-J.S., Mishima K.,
RA   Nash T.E., Moss J., Vaughan M.;
RT   "Guanine nucleotide-binding proteins in the intestinal parasite Giardia
RT   lamblia. Isolation of a gene encoding an approximately 20-kDa ADP-
RT   ribosylation factor.";
RL   J. Biol. Chem. 267:9654-9662(1992).
CC   -!- FUNCTION: GTP-binding protein involved in protein trafficking; may
CC       modulate vesicle budding and uncoating within the Golgi apparatus.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC       {ECO:0000305}.
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DR   EMBL; M86513; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; S29008; S29008.
DR   AlphaFoldDB; P26991; -.
DR   SMR; P26991; -.
DR   PRIDE; P26991; -.
DR   VEuPathDB; GiardiaDB:DHA2_7789; -.
DR   VEuPathDB; GiardiaDB:GL50581_1483; -.
DR   VEuPathDB; GiardiaDB:GL50803_007789; -.
DR   eggNOG; KOG0070; Eukaryota.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
DR   CDD; cd04150; Arf1_5_like; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR045872; Arf1-5-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR024156; Small_GTPase_ARF.
DR   InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR   PANTHER; PTHR11711; PTHR11711; 1.
DR   Pfam; PF00025; Arf; 1.
DR   PRINTS; PR00328; SAR1GTPBP.
DR   SMART; SM00178; SAR; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51417; ARF; 1.
PE   3: Inferred from homology;
KW   ER-Golgi transport; Golgi apparatus; GTP-binding; Lipoprotein; Myristate;
KW   Nucleotide-binding; Protein transport; Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000255"
FT   CHAIN           2..191
FT                   /note="ADP-ribosylation factor"
FT                   /id="PRO_0000207424"
FT   BINDING         24..31
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         67..71
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         128..131
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   LIPID           2
FT                   /note="N-myristoyl glycine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   191 AA;  21765 MW;  89C34955FDC24B8B CRC64;
     MGQGASKIFG KLFSKKEVRI LMVGLDAAGK TTILYKLMLG EVVTTVPTIG FNVETVEYKN
     INFTVWDVGG QDSIRPLWRH YYQNTDALIY VIDSADLEPK RIEDAKNELH TLLGEDELRD
     AALLVFANKQ DLPKAMSTTD LTERLGLQEL KKRDWYIQPT CARSGDGLYQ GLDWLSDYIF
     DKKNKKKGKK R