ARG33_MOUSE
ID ARG33_MOUSE Reviewed; 850 AA.
AC Q8BW86;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-JUL-2010, sequence version 3.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Rho guanine nucleotide exchange factor 33;
GN Name=Arhgef33; Synonyms=Gm941;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-460 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Oviduct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-766, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BW86-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BW86-2; Sequence=VSP_039455;
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DR EMBL; AC132910; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC161447; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK053944; BAC35600.2; -; mRNA.
DR CCDS; CCDS50189.1; -. [Q8BW86-1]
DR RefSeq; NP_001138924.1; NM_001145452.1. [Q8BW86-1]
DR RefSeq; XP_006524586.1; XM_006524523.3. [Q8BW86-1]
DR RefSeq; XP_006524587.1; XM_006524524.3. [Q8BW86-1]
DR RefSeq; XP_017173034.1; XM_017317545.1. [Q8BW86-1]
DR AlphaFoldDB; Q8BW86; -.
DR SMR; Q8BW86; -.
DR IntAct; Q8BW86; 1.
DR MINT; Q8BW86; -.
DR STRING; 10090.ENSMUSP00000063284; -.
DR iPTMnet; Q8BW86; -.
DR PhosphoSitePlus; Q8BW86; -.
DR SwissPalm; Q8BW86; -.
DR jPOST; Q8BW86; -.
DR MaxQB; Q8BW86; -.
DR PaxDb; Q8BW86; -.
DR PeptideAtlas; Q8BW86; -.
DR PRIDE; Q8BW86; -.
DR ProteomicsDB; 283258; -. [Q8BW86-1]
DR ProteomicsDB; 283259; -. [Q8BW86-2]
DR Antibodypedia; 49385; 58 antibodies from 12 providers.
DR Ensembl; ENSMUST00000068175; ENSMUSP00000063284; ENSMUSG00000054901. [Q8BW86-1]
DR Ensembl; ENSMUST00000223878; ENSMUSP00000153224; ENSMUSG00000054901. [Q8BW86-1]
DR GeneID; 381112; -.
DR KEGG; mmu:381112; -.
DR UCSC; uc012axs.1; mouse. [Q8BW86-1]
DR CTD; 100271715; -.
DR MGI; MGI:2685787; Arhgef33.
DR VEuPathDB; HostDB:ENSMUSG00000054901; -.
DR eggNOG; ENOG502RVY2; Eukaryota.
DR GeneTree; ENSGT00940000162759; -.
DR HOGENOM; CLU_018772_0_0_1; -.
DR InParanoid; Q8BW86; -.
DR OMA; LEHANMV; -.
DR OrthoDB; 229670at2759; -.
DR PhylomeDB; Q8BW86; -.
DR TreeFam; TF335627; -.
DR Reactome; R-MMU-193648; NRAGE signals death through JNK.
DR Reactome; R-MMU-416482; G alpha (12/13) signalling events.
DR BioGRID-ORCS; 381112; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Arhgef33; mouse.
DR PRO; PR:Q8BW86; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q8BW86; protein.
DR Bgee; ENSMUSG00000054901; Expressed in cerebellar cortex and 76 other tissues.
DR ExpressionAtlas; Q8BW86; baseline and differential.
DR Genevisible; Q8BW86; MM.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR Gene3D; 1.20.900.10; -; 1.
DR InterPro; IPR042849; ARHGEF33.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR PANTHER; PTHR46944; PTHR46944; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF48065; SSF48065; 1.
DR PROSITE; PS50010; DH_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Guanine-nucleotide releasing factor;
KW Methylation; Reference proteome.
FT CHAIN 1..850
FT /note="Rho guanine nucleotide exchange factor 33"
FT /id="PRO_0000342344"
FT DOMAIN 273..448
FT /note="DH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 98..209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 504..550
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 702..850
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 54..128
FT /evidence="ECO:0000255"
FT COMPBIAS 103..128
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 505..519
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 783..809
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 766
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT VAR_SEQ 1..86
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_039455"
FT CONFLICT 165
FT /note="F -> L (in Ref. 2; BAC35600)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 850 AA; 94758 MW; B18975B3390B2C0B CRC64;
MEKSKAKQGE NEHMPVNNPS TQIYQLQALA SELKTGFTEA MQELTRIQHG EYALEEKVKS
CRCSMEEKVT EMKNSLNYFK EELSNAMSMI QAITSKQEEM QQKIEQLQQE KRRESRKVKA
KKAQKEEHGA QAGPASAPAP GSAPTQGSPF RSINVPEAGL PSDDFTNMLP SQNYEKAQES
RSVHVGDSNV KGMMGPGVNP TTPESDENLK PSLSAEIQSK GHHTPGLWRQ PKEGKEWGEE
YVTKDHPDKL KDAGQGRHSS LENVLCETSL AAKRQTVALE LLESERKYVI NISLILKIKA
TFQGSDGKRN PKERSLFPGS LRYLVQQHLD LLHALQERVL KWPRQGVLGD LFLKLTNDEN
NFLDYYVAYL RDLPECISLV HVVVLKEGDE EIKSDIYTLF FHIVQRIPEY LIHLQNVLKF
TEQEHPDYYL LLVCVQRLRV FISHYTLLFQ CNEDLLIQKR KKLKKSSMAK LYKGLASQCA
NAGQDASPNA GQEAVHDSGV HSEEMLQPYP SSSSSAPAVS HLMAPAKKGQ QQQSLMESMQ
PGKPGDWELE GRKHERPESL LAPAQFCAAE QDVKALAGPL QAIPEMDFEP SSAEPLGNVE
RSLRGPPELL PDARGFVPAG YEEFEYGGEI FALPAPYDEE PFQAPALFDN CSPASSESSL
DICFLRPVSF AMEAERPEHA LQPLPKSATS PASSSGAYKL EAAQAQAHGK AKPLSRSLKE
FPRTPPAEGV APRLYSTRSS SGGRAPLKVE RAPAPHGPAA AAAASRGAPR TFFPQQRSQS
EKQTYLEEMH LEDATRFCPK EERESEQTSF SDQNPRQDQK GGFRSSFRKL FKKKNGNSTG
EDFCGPWGWW
