ID   LEUD1_METKA             Reviewed;         170 AA.
AC   Q8TX94;
DT   19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit 1;
DE            EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase 1;
DE            Short=IPMI 1;
DE   AltName: Full=Isopropylmalate isomerase 1;
GN   Name=leuD1; Synonyms=leuD_1; OrderedLocusNames=MK0781;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC       isopropylmalate, via the formation of 2-isopropylmaleate.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC         Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC         EC=4.2.1.33;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE009439; AAM01995.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8TX94; -.
DR   SMR; Q8TX94; -.
DR   STRING; 190192.MK0781; -.
DR   EnsemblBacteria; AAM01995; AAM01995; MK0781.
DR   KEGG; mka:MK0781; -.
DR   PATRIC; fig|190192.8.peg.822; -.
DR   HOGENOM; CLU_081378_1_1_2; -.
DR   OMA; GLPIIEC; -.
DR   UniPathway; UPA00048; UER00071.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd01577; IPMI_Swivel; 1.
DR   Gene3D; 3.20.19.10; -; 1.
DR   HAMAP; MF_01032; LeuD_type2; 1.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   InterPro; IPR033940; IPMI_Swivel.
DR   InterPro; IPR011827; LeuD_type2/HacB/DmdB.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   TIGRFAMs; TIGR02087; LEUD_arch; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Leucine biosynthesis; Lyase; Reference proteome.
FT   CHAIN           1..170
FT                   /note="3-isopropylmalate dehydratase small subunit 1"
FT                   /id="PRO_0000141938"
SQ   SEQUENCE   170 AA;  18686 MW;  E329CD3D366367F6 CRC64;
     MRDVIRGRAW VFGDDIDTDQ IIPGRYLTTQ DPEELAKHVM EGADPEFPEK VREGDVIVAG
     KNFGCGSSRE HAPIALKAAG IACVVTRSFA RIFYRNAINL GLPLVVCPGV DDAFEDGQGI
     EVNLREGYVR NLDTGEELEA KPLPDFMMRI LEAGGLVELI KREGPRAFEG