ID   LEUD2_METKA             Reviewed;         168 AA.
AC   Q8TW31;
DT   19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit 2;
DE            EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase 2;
DE            Short=IPMI 2;
DE   AltName: Full=Isopropylmalate isomerase 2;
GN   Name=leuD2; Synonyms=leuD_2; OrderedLocusNames=MK1206;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC       isopropylmalate, via the formation of 2-isopropylmaleate.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC         Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC         EC=4.2.1.33;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE009439; AAM02419.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8TW31; -.
DR   SMR; Q8TW31; -.
DR   STRING; 190192.MK1206; -.
DR   EnsemblBacteria; AAM02419; AAM02419; MK1206.
DR   KEGG; mka:MK1206; -.
DR   PATRIC; fig|190192.8.peg.1308; -.
DR   HOGENOM; CLU_081378_1_1_2; -.
DR   OMA; ITPGRYN; -.
DR   UniPathway; UPA00048; UER00071.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd01577; IPMI_Swivel; 1.
DR   Gene3D; 3.20.19.10; -; 1.
DR   HAMAP; MF_01032; LeuD_type2; 1.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   InterPro; IPR033940; IPMI_Swivel.
DR   InterPro; IPR011827; LeuD_type2/HacB/DmdB.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   TIGRFAMs; TIGR02087; LEUD_arch; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Leucine biosynthesis; Lyase; Reference proteome.
FT   CHAIN           1..168
FT                   /note="3-isopropylmalate dehydratase small subunit 2"
FT                   /id="PRO_0000141939"
SQ   SEQUENCE   168 AA;  18714 MW;  5C7083095952D9C6 CRC64;
     MRWSGRAHVF GDDVDTDQII PGRCLRRVSY DELGRYAMTG ADPEFPEKVR EGDVIVAGKN
     FGCGSSREQA VMALQQAGVA CVVARSFARI FYRNAINRGL PTVEAEEDPT EVVEDGNRVT
     VDLDELVLRA GSEEVPLREP PEFALQAWRE GGLLELVKKN PDKPPWRD