LEUD2_THEMA
ID LEUD2_THEMA Reviewed; 166 AA.
AC Q9WZ25;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=3-isopropylmalate dehydratase small subunit 2;
DE EC=4.2.1.33;
DE AltName: Full=Alpha-IPM isomerase 2;
DE Short=IPMI 2;
DE AltName: Full=Isopropylmalate isomerase 2;
GN Name=leuD2; OrderedLocusNames=TM_0555;
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
CC -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC isopropylmalate, via the formation of 2-isopropylmaleate.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC EC=4.2.1.33;
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 2/4.
CC -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LeuD family. LeuD type 2 subfamily.
CC {ECO:0000305}.
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DR EMBL; AE000512; AAD35640.1; -; Genomic_DNA.
DR PIR; A72363; A72363.
DR RefSeq; NP_228365.1; NC_000853.1.
DR RefSeq; WP_010865146.1; NZ_CP011107.1.
DR AlphaFoldDB; Q9WZ25; -.
DR SMR; Q9WZ25; -.
DR STRING; 243274.THEMA_01900; -.
DR EnsemblBacteria; AAD35640; AAD35640; TM_0555.
DR KEGG; tma:TM0555; -.
DR KEGG; tmw:THMA_0568; -.
DR PATRIC; fig|243274.18.peg.374; -.
DR eggNOG; COG0066; Bacteria.
DR InParanoid; Q9WZ25; -.
DR OMA; GLPIIEC; -.
DR OrthoDB; 1384217at2; -.
DR UniPathway; UPA00048; UER00071.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd01577; IPMI_Swivel; 1.
DR Gene3D; 3.20.19.10; -; 1.
DR HAMAP; MF_01032; LeuD_type2; 1.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR InterPro; IPR033940; IPMI_Swivel.
DR InterPro; IPR011824; LeuD/DmdB_bac.
DR InterPro; IPR011827; LeuD_type2/HacB/DmdB.
DR Pfam; PF00694; Aconitase_C; 1.
DR TIGRFAMs; TIGR02084; leud; 1.
DR TIGRFAMs; TIGR02087; LEUD_arch; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW Leucine biosynthesis; Lyase; Reference proteome.
FT CHAIN 1..166
FT /note="3-isopropylmalate dehydratase small subunit 2"
FT /id="PRO_0000141928"
SQ SEQUENCE 166 AA; 18451 MW; BE56CDCEB6B0D22F CRC64;
MVVKIKGKVF VFGDNVNTDE IIPARYLNTS DPQELAKYCM EDARPGFGRR DDIKGSIIVA
GENFGCGSSR EHAPVAIKAA GISCVIAKSF ARIFFRNAIN IGLPIVELKE ADEFEEGDIA
EVDLENGVVR NLTKGKEYRI RPYPEFLMKI MEAGGWLEYC LKEVGE
