ID   LEUD_ACTTI              Reviewed;          93 AA.
AC   Q44428;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit;
DE            EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase;
DE            Short=IPMI;
DE   AltName: Full=Isopropylmalate isomerase;
DE   Flags: Fragment;
GN   Name=leuD;
OS   Actinoplanes teichomyceticus.
OC   Bacteria; Actinobacteria; Micromonosporales; Micromonosporaceae;
OC   Actinoplanes.
OX   NCBI_TaxID=1867;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 31121 / DSM 43866 / BCRC 12106 / JCM 3252 / KCTC 9543 / NBRC
RC   13999 / NCIMB 12640 / NRRL B-16726 /AB 8327;
RX   PubMed=7789819; DOI=10.1016/0378-1119(95)00142-s;
RA   Castelli P., Donadio S., Marinelli F., Borghi A., Sosio M.;
RT   "Complementation of a Streptomyces lividans Leu- mutant by the Actinoplanes
RT   teichomyceticus leuC gene.";
RL   Gene 158:97-100(1995).
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC       isopropylmalate, via the formation of 2-isopropylmaleate.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC         Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC         EC=4.2.1.33;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD.
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; X84647; CAA59141.1; -; Genomic_DNA.
DR   PIR; I39700; I39700.
DR   AlphaFoldDB; Q44428; -.
DR   SMR; Q44428; -.
DR   UniPathway; UPA00048; UER00071.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.20.19.10; -; 1.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   Pfam; PF00694; Aconitase_C; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Leucine biosynthesis; Lyase.
FT   CHAIN           1..>93
FT                   /note="3-isopropylmalate dehydratase small subunit"
FT                   /id="PRO_0000141771"
FT   NON_TER         93
SQ   SEQUENCE   93 AA;  10361 MW;  780BD1B53D72A16C CRC64;
     MDKFVTHLGK IMPLRRSDVD TDQIIPAVYL KRVTRTGFED GLFSAWREDP GFVLHNPAHA
     GATILVAGPN FGTGSSRQHA VWALRDWGFK VVI