LEUD_BUCPP
ID LEUD_BUCPP Reviewed; 207 AA.
AC Q9ZEZ5;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=3-isopropylmalate dehydratase small subunit;
DE EC=4.2.1.33;
DE AltName: Full=Alpha-IPM isomerase;
DE Short=IPMI;
DE AltName: Full=Isopropylmalate isomerase;
GN Name=leuD;
OS Buchnera aphidicola subsp. Pterocomma populeum.
OG Plasmid pBPp1.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Buchnera.
OX NCBI_TaxID=98792;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9812361; DOI=10.1111/j.1574-6968.1998.tb13253.x;
RA Silva F.J., van Ham R.C.H.J., Sabater B., Latorre A.;
RT "Structure and evolution of the leucine plasmids carried by the
RT endosymbiont (Buchnera aphidicola) from aphids of the family Aphididae.";
RL FEMS Microbiol. Lett. 168:43-49(1998).
CC -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC isopropylmalate, via the formation of 2-isopropylmaleate.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC EC=4.2.1.33;
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 2/4.
CC -!- SUBUNIT: Heterodimer of LeuC and LeuD.
CC -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC {ECO:0000305}.
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DR EMBL; AJ006877; CAA07298.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9ZEZ5; -.
DR SMR; Q9ZEZ5; -.
DR UniPathway; UPA00048; UER00071.
DR GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
DR GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd01577; IPMI_Swivel; 1.
DR Gene3D; 3.20.19.10; -; 1.
DR HAMAP; MF_01031; LeuD_type1; 1.
DR InterPro; IPR004431; 3-IsopropMal_deHydase_ssu.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR InterPro; IPR033940; IPMI_Swivel.
DR Pfam; PF00694; Aconitase_C; 1.
DR TIGRFAMs; TIGR00171; leuD; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW Leucine biosynthesis; Lyase; Plasmid.
FT CHAIN 1..207
FT /note="3-isopropylmalate dehydratase small subunit"
FT /id="PRO_0000141801"
SQ SEQUENCE 207 AA; 24435 MW; 098D004E644FB27C CRC64;
MFKFTQHNGI VMPLDIANID TDIIIPKQFL QKVNKLGFGK YLFHNWRFID ENQSQINPNF
ILNIEKYKKS SILLTRDNFG CGSSREHAVW ALLDYGFKVI IAPSFSDIFY NNSFNNKLLL
ITLPNNQIDK FFNIINQNPG IYFNIDLLKN EIKVEKKIYS FQIDKFRRFC LLNGLDDIDL
TLKNIEKIDS YEKKICNFLI KRKKFVS