ID   LEUD_BUCRP              Reviewed;         207 AA.
AC   P48574;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit;
DE            EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase;
DE            Short=IPMI;
DE   AltName: Full=Isopropylmalate isomerase;
GN   Name=leuD;
OS   Buchnera aphidicola subsp. Rhopalosiphum padi.
OG   Plasmid pRPE.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=98793;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7608990; DOI=10.1007/bf00174042;
RA   Bracho A.M., Martinez-Torres D., Moya A., Latorre A.;
RT   "Discovery and molecular characterization of a plasmid localized in
RT   Buchnera sp. bacterial endosymbiont of the aphid Rhopalosiphum padi.";
RL   J. Mol. Evol. 41:67-73(1995).
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC       isopropylmalate, via the formation of 2-isopropylmaleate.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC         Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC         EC=4.2.1.33;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD.
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; X71612; CAA50618.1; -; Genomic_DNA.
DR   AlphaFoldDB; P48574; -.
DR   SMR; P48574; -.
DR   UniPathway; UPA00048; UER00071.
DR   GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd01577; IPMI_Swivel; 1.
DR   Gene3D; 3.20.19.10; -; 1.
DR   HAMAP; MF_01031; LeuD_type1; 1.
DR   InterPro; IPR004431; 3-IsopropMal_deHydase_ssu.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   InterPro; IPR033940; IPMI_Swivel.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   TIGRFAMs; TIGR00171; leuD; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Leucine biosynthesis; Lyase; Plasmid.
FT   CHAIN           1..207
FT                   /note="3-isopropylmalate dehydratase small subunit"
FT                   /id="PRO_0000141802"
SQ   SEQUENCE   207 AA;  24116 MW;  6F22D737E31134C8 CRC64;
     MFKFTEHNGV VAPLNISNID TDAIIPKQFL QKVNKIGFGK YLFHDWRFID KNQLKINPNF
     ILNNYVYKNA SILLTRENFG CGSSREHAVW SLLDYGFKVI ISSSFSDIFF NNSFNNKLLL
     VVLEKKHIDY LFNLVDTKIG ISFNVSLINK KITAGDLDIP FQIDDFQRLC LLNNWDNIDL
     TMKINHKIKS YEDNISSFLL ERQEFTS