ID   LEUD_CLOPA              Reviewed;         100 AA.
AC   P31960;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit;
DE            EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase;
DE            Short=IPMI;
DE   AltName: Full=Isopropylmalate isomerase;
DE   Flags: Fragment;
GN   Name=leuD;
OS   Clostridium pasteurianum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1501;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8173074; DOI=10.3109/10425179309020149;
RA   Oultram J.D., Loughlin M., Walmsley R.W., Gunnery S.M., Minton N.P.;
RT   "The nucleotide sequence of genes involved in the leucine biosynthetic
RT   pathway of Clostridium pasteurianum.";
RL   DNA Seq. 4:105-111(1993).
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC       isopropylmalate, via the formation of 2-isopropylmaleate.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC         Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC         EC=4.2.1.33;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD.
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; L06666; AAC41393.1; -; Genomic_DNA.
DR   AlphaFoldDB; P31960; -.
DR   SMR; P31960; -.
DR   UniPathway; UPA00048; UER00071.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.20.19.10; -; 1.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   Pfam; PF00694; Aconitase_C; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Leucine biosynthesis; Lyase.
FT   CHAIN           <1..100
FT                   /note="3-isopropylmalate dehydratase small subunit"
FT                   /id="PRO_0000141922"
FT   NON_TER         1
SQ   SEQUENCE   100 AA;  11108 MW;  13A61FCBE38BA6B8 CRC64;
     HAPIALKAAG VSCVIAKSFA RIFYRNSINI GFPILECEEA VNDAKNNHEL EVDFTTGIIK
     NITLGKEYKA QAYPQFMIDI MKNEGLINCV KNGALNSFMR