ID   LEUD_ECOLI              Reviewed;         201 AA.
AC   P30126;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 163.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit;
DE            EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase;
DE            Short=IPMI;
DE   AltName: Full=Isopropylmalate isomerase;
GN   Name=leuD; OrderedLocusNames=b0071, JW0070;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1630901; DOI=10.1093/nar/20.13.3305;
RA   Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K.,
RA   Mizobuchi K., Nakata A.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 0-
RT   2.4 min region.";
RL   Nucleic Acids Res. 20:3305-3308(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   PROTEIN SEQUENCE OF 2-5.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9600841; DOI=10.1006/jmbi.1998.1726;
RA   Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C.,
RA   Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L.,
RA   Hochstrasser D.F.;
RT   "Protein identification with N and C-terminal sequence tags in proteome
RT   projects.";
RL   J. Mol. Biol. 278:599-608(1998).
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC       isopropylmalate, via the formation of 2-isopropylmaleate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC         Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC         EC=4.2.1.33;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000250}.
CC   -!- INTERACTION:
CC       P30126; P0A6A6: leuC; NbExp=4; IntAct=EBI-1113528, EBI-1113576;
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; U00096; AAC73182.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAB96640.1; -; Genomic_DNA.
DR   PIR; S40585; S40585.
DR   RefSeq; NP_414613.1; NC_000913.3.
DR   RefSeq; WP_000818228.1; NZ_STEB01000010.1.
DR   AlphaFoldDB; P30126; -.
DR   SMR; P30126; -.
DR   BioGRID; 4262049; 4.
DR   BioGRID; 850014; 3.
DR   ComplexPortal; CPX-5159; 3-isopropylmalate dehydratase complex.
DR   DIP; DIP-10092N; -.
DR   IntAct; P30126; 8.
DR   STRING; 511145.b0071; -.
DR   SWISS-2DPAGE; P30126; -.
DR   jPOST; P30126; -.
DR   PaxDb; P30126; -.
DR   PRIDE; P30126; -.
DR   EnsemblBacteria; AAC73182; AAC73182; b0071.
DR   EnsemblBacteria; BAB96640; BAB96640; BAB96640.
DR   GeneID; 66671639; -.
DR   GeneID; 945642; -.
DR   KEGG; ecj:JW0070; -.
DR   KEGG; eco:b0071; -.
DR   PATRIC; fig|1411691.4.peg.2210; -.
DR   EchoBASE; EB1535; -.
DR   eggNOG; COG0066; Bacteria.
DR   HOGENOM; CLU_081378_0_3_6; -.
DR   InParanoid; P30126; -.
DR   OMA; AFTTHTG; -.
DR   PhylomeDB; P30126; -.
DR   BioCyc; EcoCyc:LEUD-MON; -.
DR   BioCyc; MetaCyc:LEUD-MON; -.
DR   UniPathway; UPA00048; UER00071.
DR   PRO; PR:P30126; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IMP:EcoliWiki.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009098; P:leucine biosynthetic process; IMP:EcoCyc.
DR   CDD; cd01577; IPMI_Swivel; 1.
DR   Gene3D; 3.20.19.10; -; 1.
DR   HAMAP; MF_01031; LeuD_type1; 1.
DR   InterPro; IPR004431; 3-IsopropMal_deHydase_ssu.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   InterPro; IPR033940; IPMI_Swivel.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   TIGRFAMs; TIGR00171; leuD; 1.
PE   1: Evidence at protein level;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Direct protein sequencing; Leucine biosynthesis; Lyase; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:9600841"
FT   CHAIN           2..201
FT                   /note="3-isopropylmalate dehydratase small subunit"
FT                   /id="PRO_0000141819"
SQ   SEQUENCE   201 AA;  22487 MW;  E5A98B58468E7787 CRC64;
     MAEKFIKHTG LVVPLDAANV DTDAIIPKQF LQKVTRTGFG AHLFNDWRFL DEKGQQPNPD
     FVLNFPQYQG ASILLARENF GCGSSREHAP WALTDYGFKV VIAPSFADIF YGNSFNNQLL
     PVKLSDAEVD ELFALVKANP GIHFDVDLEA QEVKAGEKTY RFTIDAFRRH CMMNGLDSIG
     LTLQHDDAIA AYEAKQPAFM N