ID   LEUD_LACLA              Reviewed;         191 AA.
AC   Q02144;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 133.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit;
DE            EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase;
DE            Short=IPMI;
DE   AltName: Full=Isopropylmalate isomerase;
GN   Name=leuD; OrderedLocusNames=LL1221; ORFNames=L0076;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NCDO 2118;
RX   PubMed=1400210; DOI=10.1128/jb.174.20.6580-6589.1992;
RA   Godon J.-J., Chopin M.-C., Ehrlich S.D.;
RT   "Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp.
RT   lactis.";
RL   J. Bacteriol. 174:6580-6589(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=8331070; DOI=10.1128/jb.175.14.4383-4390.1993;
RA   Godon J.-J., Delorme C., Bardowski J., Chopin M.-C., Ehrlich S.D.,
RA   Renault P.;
RT   "Gene inactivation in Lactococcus lactis: branched-chain amino acid
RT   biosynthesis.";
RL   J. Bacteriol. 175:4383-4390(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC       isopropylmalate, via the formation of 2-isopropylmaleate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC         Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC         EC=4.2.1.33;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD.
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: The leucine biosynthesis pathway is not functional in the
CC       dairy strain IL1403. {ECO:0000305}.
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DR   EMBL; U92974; AAB81916.1; -; Genomic_DNA.
DR   EMBL; AE005176; AAK05319.1; -; Genomic_DNA.
DR   PIR; E36889; E36889.
DR   RefSeq; NP_267377.1; NC_002662.1.
DR   RefSeq; WP_003131129.1; NC_002662.1.
DR   AlphaFoldDB; Q02144; -.
DR   SMR; Q02144; -.
DR   STRING; 272623.L0076; -.
DR   PaxDb; Q02144; -.
DR   PRIDE; Q02144; -.
DR   EnsemblBacteria; AAK05319; AAK05319; L0076.
DR   KEGG; lla:L0076; -.
DR   PATRIC; fig|272623.7.peg.1320; -.
DR   eggNOG; COG0066; Bacteria.
DR   HOGENOM; CLU_081378_0_3_9; -.
DR   OMA; AFTTHTG; -.
DR   UniPathway; UPA00048; UER00071.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd01577; IPMI_Swivel; 1.
DR   Gene3D; 3.20.19.10; -; 1.
DR   HAMAP; MF_01031; LeuD_type1; 1.
DR   InterPro; IPR004431; 3-IsopropMal_deHydase_ssu.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   InterPro; IPR033940; IPMI_Swivel.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   TIGRFAMs; TIGR00171; leuD; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Leucine biosynthesis; Lyase; Reference proteome.
FT   CHAIN           1..191
FT                   /note="3-isopropylmalate dehydratase small subunit"
FT                   /id="PRO_0000141826"
SQ   SEQUENCE   191 AA;  21849 MW;  C691BC6AAEF4E964 CRC64;
     MEKFTIYKGT SVPVMNDNID TDQIIPKQFL KAIDKKGFGK NLFYEWRYLK DYDENPDFIL
     NAPKYKKASL LISGDNFGSG SSREHAAWAL SDYGFRAIIA GSYSDIFYNN ALKNGLLPIK
     QPREVLNQLT KLSSQEEITI DLPHQLIITS LGDFHFEIDP IWKDKLINGL DDIGITLQYE
     EAISAYEQKN Q