ID   LEUD_NEIGO              Reviewed;          79 AA.
AC   Q50974;
DT   19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit;
DE            EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase;
DE            Short=IPMI;
DE   AltName: Full=Isopropylmalate isomerase;
DE   Flags: Fragment;
GN   Name=leuD;
OS   Neisseria gonorrhoeae.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=485;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=WR302;
RX   PubMed=7607490; DOI=10.1016/0378-1119(94)00649-d;
RA   Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A.;
RT   "Restriction and modification systems of Neisseria gonorrhoeae.";
RL   Gene 157:19-22(1995).
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC       isopropylmalate, via the formation of 2-isopropylmaleate.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC         Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC         EC=4.2.1.33;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; U43735; AAA86267.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q50974; -.
DR   SMR; Q50974; -.
DR   UniPathway; UPA00048; UER00071.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.20.19.10; -; 1.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Leucine biosynthesis; Lyase.
FT   CHAIN           <1..79
FT                   /note="3-isopropylmalate dehydratase small subunit"
FT                   /id="PRO_0000141841"
FT   NON_TER         1
SQ   SEQUENCE   79 AA;  9101 MW;  A3C49AA3308B33B5 CRC64;
     VDRPFKEVEA NEGYRLSIDL AEQTLTTPGG ETFTFDITEH RKHCLLNGLD EIGLTLQHAD
     KIKAFEEKRR QSQPWLFNG