ID   LEUD_PAEPO              Reviewed;         178 AA.
AC   Q9LCR5;
DT   19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit;
DE            EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase;
DE            Short=IPMI;
DE   AltName: Full=Isopropylmalate isomerase;
DE   Flags: Fragment;
GN   Name=leuD;
OS   Paenibacillus polymyxa (Bacillus polymyxa).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=1406;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10628856; DOI=10.1007/s004380051136;
RA   Ishikawa S., Kawahara S., Sekiguchi J.;
RT   "Cloning and expression of two autolysin genes, cwIU and cwIV, which are
RT   tandemly arranged on the chromosome of Bacillus polymyxa var. colistinus.";
RL   Mol. Gen. Genet. 262:738-748(1999).
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC       isopropylmalate, via the formation of 2-isopropylmaleate.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC         Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC         EC=4.2.1.33;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AB003153; BAA90647.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9LCR5; -.
DR   SMR; Q9LCR5; -.
DR   STRING; 1052684.PPM_2860; -.
DR   eggNOG; COG0066; Bacteria.
DR   UniPathway; UPA00048; UER00071.
DR   GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd01577; IPMI_Swivel; 1.
DR   Gene3D; 3.20.19.10; -; 1.
DR   InterPro; IPR004431; 3-IsopropMal_deHydase_ssu.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   InterPro; IPR033940; IPMI_Swivel.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   TIGRFAMs; TIGR00171; leuD; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Leucine biosynthesis; Lyase.
FT   CHAIN           <1..178
FT                   /note="3-isopropylmalate dehydratase small subunit"
FT                   /id="PRO_0000141851"
FT   NON_TER         1
SQ   SEQUENCE   178 AA;  20616 MW;  22F2832C778C897E CRC64;
     AIIPKQFLKR IERTGFGQFL FYEWRFDEAG NINPEFEPNK PRYAGASVLI SRANFGCGSS
     REHAPWAIMD YGFRCVIAPS FADIFYNNCF KNGILPIKLS EEQVEDLFQR TAKHDNYQLN
     VDLNEKTITD DYGLRIEFDL DEHRRQFLLQ GLDDIGLTLQ HESEILAYEQ KRAAKQGA