ID   LEUD_PYRHO              Reviewed;         163 AA.
AC   O59393;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 130.
DE   RecName: Full=3-isopropylmalate dehydratase small subunit;
DE            EC=4.2.1.33;
DE   AltName: Full=Alpha-IPM isomerase;
DE            Short=IPMI;
DE   AltName: Full=Isopropylmalate isomerase;
GN   Name=leuD; OrderedLocusNames=PH1724;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC       isopropylmalate, via the formation of 2-isopropylmaleate.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC         Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC         EC=4.2.1.33;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC       from 3-methyl-2-oxobutanoate: step 2/4.
CC   -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LeuD family. LeuD type 2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BA000001; BAA30838.1; -; Genomic_DNA.
DR   PIR; G71180; G71180.
DR   RefSeq; WP_010885788.1; NC_000961.1.
DR   PDB; 1V7L; X-ray; 1.98 A; A/B=1-163.
DR   PDBsum; 1V7L; -.
DR   AlphaFoldDB; O59393; -.
DR   SMR; O59393; -.
DR   STRING; 70601.3258155; -.
DR   EnsemblBacteria; BAA30838; BAA30838; BAA30838.
DR   GeneID; 1442569; -.
DR   KEGG; pho:PH1724; -.
DR   eggNOG; arCOG02230; Archaea.
DR   OMA; ITPGRYN; -.
DR   OrthoDB; 86621at2157; -.
DR   BRENDA; 4.2.1.33; 5244.
DR   UniPathway; UPA00048; UER00071.
DR   EvolutionaryTrace; O59393; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd01577; IPMI_Swivel; 1.
DR   Gene3D; 3.20.19.10; -; 1.
DR   HAMAP; MF_01032; LeuD_type2; 1.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   InterPro; IPR033940; IPMI_Swivel.
DR   InterPro; IPR011827; LeuD_type2/HacB/DmdB.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   TIGRFAMs; TIGR02087; LEUD_arch; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Amino-acid biosynthesis;
KW   Branched-chain amino acid biosynthesis; Leucine biosynthesis; Lyase.
FT   CHAIN           1..163
FT                   /note="3-isopropylmalate dehydratase small subunit"
FT                   /id="PRO_0000141949"
FT   STRAND          2..9
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   HELIX           16..19
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   HELIX           30..36
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   TURN            37..41
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   HELIX           45..48
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   STRAND          54..56
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   STRAND          59..61
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   HELIX           69..77
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   STRAND          81..85
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   HELIX           89..98
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   STRAND          102..105
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   STRAND          115..119
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   TURN            120..123
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   STRAND          124..127
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   STRAND          130..133
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   HELIX           139..146
FT                   /evidence="ECO:0007829|PDB:1V7L"
FT   HELIX           150..156
FT                   /evidence="ECO:0007829|PDB:1V7L"
SQ   SEQUENCE   163 AA;  18011 MW;  AEFBDDE1CBCDD266 CRC64;
     MITTGKVWKF GDDISTDEIT PGRYNLTKDP KELAKIAFIE VRPDFARNVR PGDVVVAGKN
     FGIGSSRESA ALALKALGIA GVIAESFGRI FYRNAINIGI PLLLGKTEGL KDGDLVTVNW
     ETGEVRKGDE ILMFEPLEDF LLEIVREGGI LEYIRRRGDL CIR