LEUE_ECOLI
ID LEUE_ECOLI Reviewed; 212 AA.
AC P76249; O07969; O07971;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Leucine efflux protein;
GN Name=leuE; Synonyms=yeaS; OrderedLocusNames=b1798, JW1787;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION IN LEUCINE EFFLUX, AND INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=16098526; DOI=10.1016/j.febslet.2005.07.031;
RA Kutukova E.A., Livshits V.A., Altman I.P., Ptitsyn L.R., Zyiatdinov M.H.,
RA Tokmakova I.L., Zakataeva N.P.;
RT "The yeaS (leuE) gene of Escherichia coli encodes an exporter of leucine,
RT and the Lrp protein regulates its expression.";
RL FEBS Lett. 579:4629-4634(2005).
CC -!- FUNCTION: Exporter of leucine. Can also transport its natural analog L-
CC alpha-amino-n-butyric acid and some other structurally unrelated amino
CC acids. {ECO:0000269|PubMed:16098526}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Induced by leucine, L-alpha-amino-n-butyric acid and, to a
CC lesser extent, by several other amino acids. This induction is mediated
CC by lrp. {ECO:0000269|PubMed:16098526}.
CC -!- SIMILARITY: Belongs to the Rht family. {ECO:0000305}.
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DR EMBL; U00096; AAC74868.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15593.1; -; Genomic_DNA.
DR PIR; F64940; F64940.
DR RefSeq; NP_416312.1; NC_000913.3.
DR RefSeq; WP_000457206.1; NZ_LN832404.1.
DR AlphaFoldDB; P76249; -.
DR BioGRID; 4260333; 34.
DR STRING; 511145.b1798; -.
DR TCDB; 2.A.76.1.5; the resistance to homoserine/threonine (rhtb) family.
DR PaxDb; P76249; -.
DR PRIDE; P76249; -.
DR EnsemblBacteria; AAC74868; AAC74868; b1798.
DR EnsemblBacteria; BAA15593; BAA15593; BAA15593.
DR GeneID; 946157; -.
DR KEGG; ecj:JW1787; -.
DR KEGG; eco:b1798; -.
DR PATRIC; fig|1411691.4.peg.455; -.
DR EchoBASE; EB3278; -.
DR eggNOG; COG1280; Bacteria.
DR HOGENOM; CLU_079569_3_1_6; -.
DR InParanoid; P76249; -.
DR OMA; AGVWCGD; -.
DR PhylomeDB; P76249; -.
DR BioCyc; EcoCyc:G6984-MON; -.
DR BioCyc; MetaCyc:G6984-MON; -.
DR PRO; PR:P76249; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015190; F:L-leucine transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0006865; P:amino acid transport; IBA:GO_Central.
DR GO; GO:0015820; P:leucine transport; IMP:EcoCyc.
DR InterPro; IPR001123; LeuE-type.
DR PANTHER; PTHR30086; PTHR30086; 1.
DR Pfam; PF01810; LysE; 1.
DR PIRSF; PIRSF006324; LeuE; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..212
FT /note="Leucine efflux protein"
FT /id="PRO_0000094742"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 212 AA; 23201 MW; 342E0DF348C9AD9A CRC64;
MFAEYGVLNY WTYLVGAIFI VLVPGPNTLF VLKNSVSSGM KGGYLAACGV FIGDAVLMFL
AWAGVATLIK TTPILFNIVR YLGAFYLLYL GSKILYATLK GKNSEAKSDE PQYGAIFKRA
LILSLTNPKA ILFYVSFFVQ FIDVNAPHTG ISFFILAATL ELVSFCYLSF LIISGAFVTQ
YIRTKKKLAK VGNSLIGLMF VGFAARLATL QS
