LFG2_ARATH
ID LFG2_ARATH Reviewed; 239 AA.
AC Q9M1V9;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Protein LIFEGUARD 2 {ECO:0000303|PubMed:23888068};
DE Short=AtLFG2 {ECO:0000303|PubMed:23888068};
DE AltName: Full=Protein BRZ-INSENSITIVE-LONG HYPOCOTYLS 4 {ECO:0000303|PubMed:19202280};
GN Name=LFG2 {ECO:0000303|PubMed:23888068};
GN Synonyms=BIL4 {ECO:0000303|PubMed:19202280};
GN OrderedLocusNames=At3g63310 {ECO:0000312|Araport:AT3G63310};
GN ORFNames=MAA21.11 {ECO:0000312|EMBL:CAB86432.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=17064408; DOI=10.1186/gb-2006-7-10-r96;
RA Moriyama E.N., Strope P.K., Opiyo S.O., Chen Z., Jones A.M.;
RT "Mining the Arabidopsis thaliana genome for highly-divergent seven
RT transmembrane receptors.";
RL Genome Biol. 7:R96.1-R96.9(2006).
RN [5]
RP FUNCTION, REGULATION BY BRZ, TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=19202280; DOI=10.1271/bbb.80752;
RA Yamagami A., Nakazawa M., Matsui M., Tujimoto M., Sakuta M., Asami T.,
RA Nakano T.;
RT "Chemical genetics reveal the novel transmembrane protein BIL4, which
RT mediates plant cell elongation in brassinosteroid signaling.";
RL Biosci. Biotechnol. Biochem. 73:415-421(2009).
RN [6]
RP FUNCTION (MICROBIAL INFECTION), DISRUPTION PHENOTYPE, TISSUE SPECIFICITY,
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=23888068; DOI=10.1093/jxb/ert217;
RA Weis C., Hueckelhoven R., Eichmann R.;
RT "LIFEGUARD proteins support plant colonization by biotrophic powdery mildew
RT fungi.";
RL J. Exp. Bot. 64:3855-3867(2013).
CC -!- FUNCTION: Regulates the brassinosteroid (BR) signaling pathway that
CC mediates cell elongation and organ morphogenesis (PubMed:19202280).
CC {ECO:0000269|PubMed:19202280}.
CC -!- FUNCTION: (Microbial infection) Facilitates the development of the
CC powdery mildew fungus E.cruciferarum. {ECO:0000269|PubMed:23888068}.
CC -!- FUNCTION: (Microbial infection) May prevent cell death upon A.alternata
CC f.sp. lycopersici (AAL) toxin treatment. {ECO:0000269|PubMed:23888068}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, roots, leaves,
CC inflorescences and flowers. {ECO:0000269|PubMed:19202280,
CC ECO:0000269|PubMed:23888068}.
CC -!- INDUCTION: Repressed by Brz, a triazole compound that acts as a
CC brassinosteroid (BR)-specific inhibitor. {ECO:0000269|PubMed:19202280}.
CC -!- DISRUPTION PHENOTYPE: Delayed development of the powdery mildew fungus
CC E.cruciferarum. Increased cell death upon A.alternata f.sp. lycopersici
CC (AAL) toxin treatment. {ECO:0000269|PubMed:23888068}.
CC -!- SIMILARITY: Belongs to the BI1 family. {ECO:0000305}.
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DR EMBL; AL138648; CAB86432.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE80466.1; -; Genomic_DNA.
DR EMBL; BT020236; AAV74230.1; -; mRNA.
DR EMBL; BT021132; AAX22267.1; -; mRNA.
DR PIR; T48120; T48120.
DR RefSeq; NP_191890.1; NM_116196.3.
DR AlphaFoldDB; Q9M1V9; -.
DR SMR; Q9M1V9; -.
DR IntAct; Q9M1V9; 19.
DR STRING; 3702.AT3G63310.1; -.
DR TCDB; 1.A.14.3.13; the calcium transporter a (cata) (formerly the testis-enhanced gene transfer (tegt) family.
DR iPTMnet; Q9M1V9; -.
DR PaxDb; Q9M1V9; -.
DR PRIDE; Q9M1V9; -.
DR ProteomicsDB; 238456; -.
DR EnsemblPlants; AT3G63310.1; AT3G63310.1; AT3G63310.
DR GeneID; 825506; -.
DR Gramene; AT3G63310.1; AT3G63310.1; AT3G63310.
DR KEGG; ath:AT3G63310; -.
DR Araport; AT3G63310; -.
DR TAIR; locus:2077309; AT3G63310.
DR eggNOG; KOG2322; Eukaryota.
DR HOGENOM; CLU_058671_0_1_1; -.
DR InParanoid; Q9M1V9; -.
DR OMA; NRSHQAE; -.
DR OrthoDB; 1290306at2759; -.
DR PhylomeDB; Q9M1V9; -.
DR PRO; PR:Q9M1V9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1V9; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IMP:UniProtKB.
DR GO; GO:0060548; P:negative regulation of cell death; IMP:UniProtKB.
DR GO; GO:1905421; P:regulation of plant organ morphogenesis; IMP:UniProtKB.
DR GO; GO:0009826; P:unidimensional cell growth; IMP:TAIR.
DR InterPro; IPR006214; Bax_inhibitor_1-related.
DR PANTHER; PTHR23291; PTHR23291; 1.
DR Pfam; PF01027; Bax1-I; 1.
PE 2: Evidence at transcript level;
KW Brassinosteroid signaling pathway; Developmental protein; Membrane;
KW Plant defense; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..239
FT /note="Protein LIFEGUARD 2"
FT /id="PRO_0000441630"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 239 AA; 26908 MW; 5E40C734D952C43B CRC64;
MWNQKHDLES AQTPLYPMMS ESPELRWSFI RKVYSIISIQ LLVTIAVAAT VVKVHSISVF
FTTTTAGFAL YILLILTPLI VMCPLYYYHQ KHPVNYLLLG IFTVALAFAV GLTCAFTSGK
VILESVILTA VVVISLTLYT FWAAKRGHDF NFLGPFLFGA VIVLMVFSFI QILFPLGKIS
VMIYGCLASI IFCGYIVYDT DNLIKRHSYD EYIWAAVSLY LDVINLFLSL LTLLRAVDS