LFTY2_MOUSE
ID LFTY2_MOUSE Reviewed; 368 AA.
AC P57785; Q8BMF7;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Left-right determination factor 2;
DE AltName: Full=Left-right determination factor B;
DE AltName: Full=Protein lefty-2;
DE AltName: Full=Protein lefty-B;
DE Flags: Precursor;
GN Name=Lefty2; Synonyms=Leftb;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=9348041; DOI=10.1046/j.1365-2443.1997.1400338.x;
RA Meno C., Ito Y., Saijoh Y., Matsuda Y., Tashiro K., Kuhara S., Hamada H.;
RT "Two closely-related left-right asymmetrically expressed genes, lefty-1 and
RT lefty-2: their distinct expression domains, chromosomal linkage and direct
RT neuralizing activity in Xenopus embryos.";
RL Genes Cells 2:513-524(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=129/Sv X 129SvCp; TISSUE=Embryonic stem cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Required for left-right asymmetry determination of organ
CC systems in mammals.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- DEVELOPMENTAL STAGE: At the primitive streak stage (7.0 dpc), expressed
CC in the emerging mesoderm. By 8.0 dpc, expressed exclusively on the left
CC side of developing embryos with expression predominantly in the
CC lateral-plate mesoderm (LPM). Weak expression in the prospective floor
CC plate (PFP).
CC -!- PTM: The processing of the protein may also occur at the second R-X-X-R
CC site located at AA 132-135. Processing appears to be regulated in a
CC cell-type specific manner.
CC -!- SIMILARITY: Belongs to the TGF-beta family. {ECO:0000305}.
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DR EMBL; AK031621; BAC27483.1; -; mRNA.
DR EMBL; BC066224; AAH66224.1; -; mRNA.
DR CCDS; CCDS15575.1; -.
DR RefSeq; NP_796073.1; NM_177099.4.
DR AlphaFoldDB; P57785; -.
DR STRING; 10090.ENSMUSP00000082952; -.
DR GlyGen; P57785; 1 site.
DR iPTMnet; P57785; -.
DR PhosphoSitePlus; P57785; -.
DR PaxDb; P57785; -.
DR PeptideAtlas; P57785; -.
DR PRIDE; P57785; -.
DR DNASU; 320202; -.
DR Ensembl; ENSMUST00000085797; ENSMUSP00000082952; ENSMUSG00000066652.
DR GeneID; 320202; -.
DR KEGG; mmu:320202; -.
DR UCSC; uc007dwu.1; mouse.
DR CTD; 7044; -.
DR MGI; MGI:2443573; Lefty2.
DR VEuPathDB; HostDB:ENSMUSG00000066652; -.
DR eggNOG; KOG3900; Eukaryota.
DR GeneTree; ENSGT00390000010056; -.
DR HOGENOM; CLU_064098_0_0_1; -.
DR InParanoid; P57785; -.
DR OMA; SCAWDGV; -.
DR OrthoDB; 751948at2759; -.
DR PhylomeDB; P57785; -.
DR TreeFam; TF106462; -.
DR BioGRID-ORCS; 320202; 1 hit in 72 CRISPR screens.
DR PRO; PR:P57785; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P57785; protein.
DR Bgee; ENSMUSG00000066652; Expressed in mesoderm and 29 other tissues.
DR Genevisible; P57785; MM.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0005160; F:transforming growth factor beta receptor binding; IEA:InterPro.
DR GO; GO:0030509; P:BMP signaling pathway; IBA:GO_Central.
DR GO; GO:0010862; P:positive regulation of pathway-restricted SMAD protein phosphorylation; IBA:GO_Central.
DR GO; GO:0060395; P:SMAD protein signal transduction; IBA:GO_Central.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR003942; LRDF.
DR InterPro; IPR001839; TGF-b_C.
DR InterPro; IPR001111; TGF-b_propeptide.
DR InterPro; IPR015615; TGF-beta-rel.
DR InterPro; IPR017948; TGFb_CS.
DR PANTHER; PTHR11848; PTHR11848; 1.
DR Pfam; PF00019; TGF_beta; 1.
DR Pfam; PF00688; TGFb_propeptide; 1.
DR PIRSF; PIRSF037402; TGFb4; 1.
DR PRINTS; PR01427; TGFBETA4.
DR SMART; SM00204; TGFB; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
DR PROSITE; PS00250; TGF_BETA_1; 1.
DR PROSITE; PS51362; TGF_BETA_2; 1.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Cytokine; Developmental protein;
KW Disulfide bond; Glycoprotein; Growth factor; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000250"
FT PROPEP 22..77
FT /note="Or 135"
FT /evidence="ECO:0000255"
FT /id="PRO_0000033812"
FT CHAIN 78..368
FT /note="Left-right determination factor 2"
FT /id="PRO_0000033813"
FT CARBOHYD 158
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 253..266
FT /evidence="ECO:0000250"
FT DISULFID 265..318
FT /evidence="ECO:0000250"
FT DISULFID 295..353
FT /evidence="ECO:0000250"
FT DISULFID 299..355
FT /evidence="ECO:0000250"
FT CONFLICT 82
FT /note="F -> L (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 368 AA; 41175 MW; 5BEE8B2F1305A44B CRC64;
MKSLWLCWAL WVLPLAGPGA AMTEEQVLSS LLQQLQLSQA PTLDSADVEE MAIPTHVRSQ
YVALLQGSHA DRSRGKRFSQ NFREVAGRFL MSETSTHLLV FGMEQRLPPN SELVQAVLRL
FQEPVPRTAL RRFERLSPHS ARARVTIEWL RVREDGSNRT ALIDSRLVSI HESGWKAFDV
TEAVNFWQQL SRPRQPLLLQ VSVQREHLGP GTWSAHKLVR FAAQGTPDGK GQGEPQLELH
TLDLKDYGAQ GNCDPEVPVT EGTRCCRQEM YLDLQGMKWA ENWILEPPGF LTYECVGSCL
QLPESLTIGW PFLGPRQCVA SEMTSLPMIV SVKEGGRTRP QVVSLPNMRV QTCSCASDGA
LIPRGIDL
