LF_ORYSJ
ID LF_ORYSJ Reviewed; 416 AA.
AC A0A0P0WQ90; C7J2H7; Q5TKP7;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2016, sequence version 1.
DT 25-MAY-2022, entry version 39.
DE RecName: Full=Transcription factor LATE FLOWERING {ECO:0000303|PubMed:21549224};
DE Short=OsLF {ECO:0000303|PubMed:21549224};
DE AltName: Full=Basic helix-loop-helix protein 119 {ECO:0000303|PubMed:16896230};
DE Short=OsbHLH119 {ECO:0000303|PubMed:16896230};
GN Name=LF {ECO:0000303|PubMed:21549224};
GN Synonyms=BHLH119 {ECO:0000303|PubMed:16896230};
GN OrderedLocusNames=Os05g0541400 {ECO:0000312|EMBL:BAS95124.1},
GN LOC_Os05g46370 {ECO:0000305};
GN ORFNames=OJ1362_G11.11 {ECO:0000312|EMBL:AAV67820.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16896230; DOI=10.1104/pp.106.080580;
RA Li X., Duan X., Jiang H., Sun Y., Tang Y., Yuan Z., Guo J., Liang W.,
RA Chen L., Yin J., Ma H., Wang J., Zhang D.;
RT "Genome-wide analysis of basic/helix-loop-helix transcription factor family
RT in rice and Arabidopsis.";
RL Plant Physiol. 141:1167-1184(2006).
RN [6]
RP FUNCTION, INTERACTION WITH PIL13 AND PIL15, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Zhonghua 11;
RX PubMed=21549224; DOI=10.1016/j.nbt.2011.04.006;
RA Zhao X.L., Shi Z.Y., Peng L.T., Shen G.Z., Zhang J.L.;
RT "An atypical HLH protein OsLF in rice regulates flowering time and
RT interacts with OsPIL13 and OsPIL15.";
RL New Biotechnol. 28:788-797(2011).
CC -!- FUNCTION: Transcription factor involved in the negative regulation of
CC flowering. May be involved in the repression of the flowering factor GI
CC and HD1 by interacting with PIL13 and PIL15 and competing with PRR1.
CC Possesses transactivation activity in yeast.
CC {ECO:0000269|PubMed:21549224}.
CC -!- SUBUNIT: Interacts with PIL13 and PIL15. {ECO:0000269|PubMed:21549224}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:21549224}.
CC -!- MISCELLANEOUS: The gain-of-function mutant A654 (T-DNA tagging) exhibit
CC late flowering phenotype under short day (SD) and long day (LD)
CC conditions. {ECO:0000269|PubMed:21549224}.
CC -!- SIMILARITY: Belongs to the bHLH protein family. {ECO:0000305}.
CC -!- CAUTION: Contains a degenerate basic motif not likely to bind DNA.
CC {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAH93234.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC104713; AAV67820.1; -; Genomic_DNA.
DR EMBL; AP008211; BAH93234.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014961; BAS95124.1; -; Genomic_DNA.
DR RefSeq; XP_015638480.1; XM_015782994.1.
DR AlphaFoldDB; A0A0P0WQ90; -.
DR SMR; A0A0P0WQ90; -.
DR STRING; 4530.OS05T0541400-00; -.
DR PaxDb; A0A0P0WQ90; -.
DR EnsemblPlants; Os05t0541400-01; Os05t0541400-01; Os05g0541400.
DR GeneID; 9269440; -.
DR Gramene; Os05t0541400-01; Os05t0541400-01; Os05g0541400.
DR KEGG; osa:9269440; -.
DR eggNOG; ENOG502QR69; Eukaryota.
DR HOGENOM; CLU_053770_0_0_1; -.
DR OMA; DRFAFPN; -.
DR OrthoDB; 1722351at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0048586; P:regulation of long-day photoperiodism, flowering; IMP:UniProtKB.
DR GO; GO:0048587; P:regulation of short-day photoperiodism, flowering; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR045843; IND-like.
DR PANTHER; PTHR45914; PTHR45914; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..416
FT /note="Transcription factor LATE FLOWERING"
FT /id="PRO_0000444468"
FT DOMAIN 303..352
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 176..226
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 276..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 303..316
FT /note="Basic motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 317..352
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT COMPBIAS 199..213
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 265
FT /note="I -> V (in Ref. 2; BAH93234 and 1; AAV67820)"
FT /evidence="ECO:0000305"
FT CONFLICT 270
FT /note="A -> P (in Ref. 2; BAH93234 and 1; AAV67820)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 416 AA; 42671 MW; 6128394EBC8E8DE4 CRC64;
MYMDAFGWSA PAAPCQPSCG PGGDDDDDVL LAAVLGASFE LHSLVDGGGN GAAGAVRSDD
AYGLDVDLPS HQMSLLRCQD GLSALHGDAS PTAAAAAFLD SVDVLPVPAI AGATHDDGGL
LDRFAFPNVA ETTTVQAAAS NTAFSGYSSN TTGGGNISSG ESNTYTEVAS TPCAVSTTTT
TTALPPSKRK LPEKYPVVGT SPTTKTTTTS ETAAERRSTK RGAGGSSSIT FGGGCHGAGA
AAALLGYGRG YEPDTEAIAQ VKEMIYRAAA MRPVTLGGPA SASDPSSRPP PPPQRPRRKN
VRISSDPQTV AARLRRERVS ERLRVLQRLV PGGSKMDTAT MLDEAASYLK FLKSQLEALE
TLGNGNGNGN LLHHGYYTGS RNATATAATG SSNSTVLAFG RDGLAGFVKS NRNLQL
