LGB1_LUPLU
ID LGB1_LUPLU Reviewed; 154 AA.
AC P02239;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Leghemoglobin-1;
DE AltName: Full=Leghemoglobin I;
OS Lupinus luteus (European yellow lupine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC genistoids sensu lato; core genistoids; Genisteae; Lupinus.
OX NCBI_TaxID=3873;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3628011; DOI=10.1093/nar/15.16.6742;
RA Konieczny A.;
RT "Nucleotide sequence of lupin leghemoglobin I cDNA.";
RL Nucleic Acids Res. 15:6742-6742(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Ventus; TISSUE=Root nodule;
RA Strozycki P.S.P.;
RL Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Ventus;
RA Strozycki P.M., Karlowski W.M., Legocki A.B.;
RT "Yellow lupine gene coding for leghemoglobin I.";
RL (er) Plant Gene Register PGR98-017(1998).
RN [4]
RP PROTEIN SEQUENCE OF 2-154.
RC TISSUE=Root nodule;
RA Egorov T.A., Feigina M.Y., Kazakov V.K., Shakhparonov M.I., Mimaleva S.I.,
RA Ovchinnikov Y.A.;
RT "The complete amino acid sequence of the leghemoglobin I from yellow lupin
RT root nodules.";
RL Bioorg. Khim. 2:125-128(1976).
CC -!- FUNCTION: Provides oxygen to the bacteroids. This role is essential for
CC symbiotic nitrogen fixation.
CC -!- SUBUNIT: Monomer.
CC -!- TISSUE SPECIFICITY: Root nodules.
CC -!- SIMILARITY: Belongs to the plant globin family. {ECO:0000305}.
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DR EMBL; Y00401; CAA68462.1; -; mRNA.
DR EMBL; X77043; CAA54332.1; -; mRNA.
DR EMBL; U50083; AAC04853.1; -; Genomic_DNA.
DR PIR; A26808; GPYL.
DR AlphaFoldDB; P02239; -.
DR SMR; P02239; -.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019825; F:oxygen binding; IEA:InterPro.
DR GO; GO:0005344; F:oxygen carrier activity; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.10; -; 1.
DR InterPro; IPR000971; Globin.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012292; Globin/Proto.
DR InterPro; IPR001032; Leghaemoglobin.
DR InterPro; IPR019824; Leghaemoglobin_Fe_BS.
DR PANTHER; PTHR22924; PTHR22924; 1.
DR Pfam; PF00042; Globin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR PROSITE; PS01033; GLOBIN; 1.
DR PROSITE; PS00208; PLANT_GLOBIN; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Heme; Iron; Metal-binding; Nitrogen fixation;
KW Oxygen transport; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.4"
FT CHAIN 2..154
FT /note="Leghemoglobin-1"
FT /id="PRO_0000192984"
FT BINDING 64
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="distal binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00238"
FT BINDING 98
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="proximal binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00238"
FT CONFLICT 80
FT /note="Q -> E (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="E -> G (in Ref. 1; CAA68462)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 154 AA; 16753 MW; 58101C830CB21F14 CRC64;
MGVLTDVQVA LVKSSFEEFN ANIPKNTHRF FTLVLEIAPG AKDLFSFLKG SSEVPQNNPD
LQAHAGKVFK LTYEAAIQLQ VNGAVASDAT LKSLGSVHVS KGVVDAHFPV VKEAILKTIK
EVVGDKWSEE LNTAWTIAYD ELAIIIKKEM KDAA
