LGB2_SOYBN
ID LGB2_SOYBN Reviewed; 145 AA.
AC P02236;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Leghemoglobin C2;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6285303; DOI=10.1093/nar/10.11.3487;
RA Wiborg O., Hyldig-Nielsen J.J., Jensen E.O., Paludan K., Marcker K.A.;
RT "The nucleotide sequences of two leghemoglobin genes from soybean.";
RL Nucleic Acids Res. 10:3487-3494(1982).
RN [2]
RP PROTEIN SEQUENCE OF 2-145.
RA Sievers G., Huhtala M.-L., Ellfolk N.;
RT "The primary structure of soybean (Glycine max) leghemoglobin c.";
RL Acta Chem. Scand. B 32:380-386(1978).
RN [3]
RP SEQUENCE REVISION.
RA Ellfolk N.;
RL Submitted (NOV-1982) to the PIR data bank.
RN [4]
RP PROTEIN SEQUENCE OF 2-145.
RX PubMed=561001; DOI=10.1016/0014-5793(77)80398-0;
RA Hurrell J.G.R., Leach S.J.;
RT "The amino acid sequence of soybean leghaemoglobin c2.";
RL FEBS Lett. 80:23-26(1977).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 100-145.
RA Sullivan D., Brisson N., Goodchild B., Verma D.P.S.;
RT "Molecular cloning and organization of two leghemoglobin sequences of
RT soybean.";
RL Nature 289:516-518(1981).
CC -!- FUNCTION: Provides oxygen to the bacteroids. This role is essential for
CC symbiotic nitrogen fixation.
CC -!- SUBUNIT: Monomer.
CC -!- TISSUE SPECIFICITY: Root nodules.
CC -!- SIMILARITY: Belongs to the plant globin family. {ECO:0000305}.
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DR EMBL; J01301; AAA33980.1; -; Genomic_DNA.
DR EMBL; V00457; CAA23735.1; -; Genomic_DNA.
DR RefSeq; NP_001235248.1; NM_001248319.2.
DR AlphaFoldDB; P02236; -.
DR SMR; P02236; -.
DR STRING; 3847.GLYMA20G33290.1; -.
DR PRIDE; P02236; -.
DR ProMEX; P02236; -.
DR EnsemblPlants; KRG92101; KRG92101; GLYMA_20G191200.
DR GeneID; 100527379; -.
DR Gramene; KRG92101; KRG92101; GLYMA_20G191200.
DR KEGG; gmx:100527379; -.
DR eggNOG; KOG3378; Eukaryota.
DR HOGENOM; CLU_003827_11_2_1; -.
DR InParanoid; P02236; -.
DR OMA; TSWEVAY; -.
DR OrthoDB; 1379813at2759; -.
DR Proteomes; UP000008827; Chromosome 20.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019825; F:oxygen binding; IEA:InterPro.
DR GO; GO:0005344; F:oxygen carrier activity; IEA:UniProtKB-KW.
DR GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.10; -; 1.
DR InterPro; IPR000971; Globin.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012292; Globin/Proto.
DR InterPro; IPR001032; Leghaemoglobin.
DR InterPro; IPR019824; Leghaemoglobin_Fe_BS.
DR PANTHER; PTHR22924; PTHR22924; 1.
DR Pfam; PF00042; Globin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR PROSITE; PS01033; GLOBIN; 1.
DR PROSITE; PS00208; PLANT_GLOBIN; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Heme; Iron; Metal-binding; Nitrogen fixation;
KW Nodulation; Oxygen transport; Reference proteome; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:561001, ECO:0000269|Ref.2"
FT CHAIN 2..145
FT /note="Leghemoglobin C2"
FT /id="PRO_0000193004"
FT BINDING 62
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="distal binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00238"
FT BINDING 93
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="proximal binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00238"
FT VARIANT 9
FT /note="E -> D"
FT VARIANT 98
FT /note="I -> V"
FT CONFLICT 32
FT /note="T -> N (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 39..41
FT /note="PAA -> FAV (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 49
FT /note="S -> A (in Ref. 2; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 53
FT /note="D -> N (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="S -> T (in Ref. 2; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 68
FT /note="G -> A (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 80..81
FT /note="Missing (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 80
FT /note="N -> S (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 89
FT /note="L -> S (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 100..102
FT /note="DPQ -> NPE (in Ref. 2; AA sequence and 4; AA
FT sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 106
FT /note="Missing (in Ref. 2; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 145 AA; 15525 MW; 5A903EF234935F88 CRC64;
MGAFTEKQEA LVSSSFEAFK ANIPQYSVVF YTSILEKAPA AKDLFSFLSN GVDPSNPKLT
GHAEKLFGLV RDSAGQLKAN GTVVADAALG SIHAQKAITD PQFVVVKEAL LKTIKEAVGD
KWSDELSSAW EVAYDELAAA IKKAF
