ID   LGB2_VICFA              Reviewed;         148 AA.
AC   P93848;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Leghemoglobin 29;
DE   AltName: Full=VfLb29;
GN   Name=LB29;
OS   Vicia faba (Broad bean) (Faba vulgaris).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Vicia.
OX   NCBI_TaxID=3906;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Kleine Thueringer; TISSUE=Root nodule;
RX   PubMed=9002275; DOI=10.1094/mpmi.1997.10.1.124;
RA   Fruehling M., Roussel H., Gianinazzi-Pearson V., Puehler A., Perlick A.M.;
RT   "The Vicia faba leghemoglobin gene VfLb29 is induced in root nodules and in
RT   roots colonized by the arbuscular mycorrhizal fungus Glomus fasciculatum.";
RL   Mol. Plant Microbe Interact. 10:124-131(1997).
CC   -!- FUNCTION: Provides oxygen to the bacteroids. This role is essential for
CC       symbiotic nitrogen fixation.
CC   -!- TISSUE SPECIFICITY: Root nodules.
CC   -!- SIMILARITY: Belongs to the plant globin family. {ECO:0000305}.
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DR   EMBL; Z54160; CAA90871.1; -; mRNA.
DR   PIR; T12131; T12131.
DR   AlphaFoldDB; P93848; -.
DR   SMR; P93848; -.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019825; F:oxygen binding; IEA:InterPro.
DR   GO; GO:0005344; F:oxygen carrier activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.490.10; -; 1.
DR   InterPro; IPR000971; Globin.
DR   InterPro; IPR009050; Globin-like_sf.
DR   InterPro; IPR012292; Globin/Proto.
DR   InterPro; IPR001032; Leghaemoglobin.
DR   PANTHER; PTHR22924; PTHR22924; 1.
DR   Pfam; PF00042; Globin; 1.
DR   SUPFAM; SSF46458; SSF46458; 1.
DR   PROSITE; PS01033; GLOBIN; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Metal-binding; Nitrogen fixation; Oxygen transport; Transport.
FT   CHAIN           1..148
FT                   /note="Leghemoglobin 29"
FT                   /id="PRO_0000193007"
FT   BINDING         63
FT                   /ligand="heme b"
FT                   /ligand_id="ChEBI:CHEBI:60344"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="distal binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00238"
FT   BINDING         95
FT                   /ligand="heme b"
FT                   /ligand_id="ChEBI:CHEBI:60344"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="proximal binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00238"
SQ   SEQUENCE   148 AA;  16548 MW;  6A74DA1E933BACBB CRC64;
     MEFTLRQEAL VNSSWEAFNQ NLPLFSVLFY TFILEKAPIA KNMFSVLKDA NEIPLANPSI
     NAHTEMVFEM VRDAAAQLQT TGQVVLGDTT LGVVHTQKRV DGLHFMVVKE ALLKTIKEAV
     GDKWSEELSN AWEIAYDGLA VAIMKEMS