LGB3_MEDSA
ID LGB3_MEDSA Reviewed; 146 AA.
AC P14962; Q42929;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Leghemoglobin-3;
DE AltName: Full=Leghemoglobin III;
OS Medicago sativa (Alfalfa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3879;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Cardinal; TISSUE=Root nodule;
RX PubMed=3575105; DOI=10.1093/nar/15.8.3620;
RA Kiss G.B., Vegh Z., Vincze E.;
RT "Nucleotide sequence of a cDNA clone encoding leghemoglobin III (LbIII)
RT from Medicago sativa.";
RL Nucleic Acids Res. 15:3620-3620(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1888894; DOI=10.1007/bf00017926;
RA Davidowitz E.J., Creissen G., Vincze E., Kiss G.B., Lang-Unnasch N.;
RT "Sequence analysis of alfalfa (Medicago sativa) leghemoglobin cDNA and
RT genomic clones.";
RL Plant Mol. Biol. 16:161-165(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 16-146.
RC STRAIN=cv. Hybrid 11 X 8; TISSUE=Root nodule;
RX AGRICOLA=IND91035192; DOI=10.1007/BF00019516;
RA Barker D.G., Gallusci P., Lullien V., Khan H., Gherardi M., Huguet T.;
RT "Identification of two groups of leghemoglobin genes in alfalfa (Medicago
RT sativa) and a study of their expression during root nodule development.";
RL Plant Mol. Biol. 11:761-772(1988).
CC -!- FUNCTION: Provides oxygen to the bacteroids. This role is essential for
CC symbiotic nitrogen fixation.
CC -!- SUBUNIT: Monomer.
CC -!- TISSUE SPECIFICITY: Root nodules.
CC -!- SIMILARITY: Belongs to the plant globin family. {ECO:0000305}.
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DR EMBL; X14310; CAA32491.1; -; mRNA.
DR EMBL; M32883; AAA32659.1; -; Genomic_DNA.
DR EMBL; Y00134; CAA68328.1; -; mRNA.
DR EMBL; M36101; AAA32658.1; -; mRNA.
DR PIR; A29282; A29282.
DR PIR; S08507; S08507.
DR AlphaFoldDB; P14962; -.
DR SMR; P14962; -.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019825; F:oxygen binding; IEA:InterPro.
DR GO; GO:0005344; F:oxygen carrier activity; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.10; -; 1.
DR InterPro; IPR000971; Globin.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012292; Globin/Proto.
DR InterPro; IPR001032; Leghaemoglobin.
DR InterPro; IPR019824; Leghaemoglobin_Fe_BS.
DR PANTHER; PTHR22924; PTHR22924; 1.
DR Pfam; PF00042; Globin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR PROSITE; PS01033; GLOBIN; 1.
DR PROSITE; PS00208; PLANT_GLOBIN; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Metal-binding; Nitrogen fixation; Oxygen transport; Transport.
FT CHAIN 1..146
FT /note="Leghemoglobin-3"
FT /id="PRO_0000192987"
FT BINDING 61
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="distal binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00238"
FT BINDING 93
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="proximal binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00238"
FT CONFLICT 16
FT /note="E -> K (in Ref. 3; CAA32491/AAA32658)"
FT /evidence="ECO:0000305"
FT CONFLICT 47
FT /note="Missing (in Ref. 2; AAA32659)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 146 AA; 15741 MW; DA449DAF25B7ED69 CRC64;
MGFTDKQEAL VNSSWESFKQ NPGNSVLFYT IILEKAPAAK GMFSFLKDSA GVQDSPKLQS
HAEKVFGMVR DSAAQLRATG GVVLGDATLG AIHIQKGVVD PHFAVVKEAL LKTIKEVSGD
KWSEELNTAW EVAYDALATA IKKAMV
