LGI2_MOUSE
ID LGI2_MOUSE Reviewed; 550 AA.
AC Q8K4Z0; Q80T76;
DT 03-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Leucine-rich repeat LGI family member 2;
DE AltName: Full=Leucine-rich glioma-inactivated protein 2;
DE Flags: Precursor;
GN Name=Lgi2; Synonyms=Kiaa1916;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J;
RX PubMed=12217514; DOI=10.1016/s0968-0004(02)02163-1;
RA Staub E., Perez-Tur J., Siebert R., Nobile C., Moschonas N.K., Deloukas P.,
RA Hinzmann B.;
RT "The novel EPTP repeat defines a superfamily of proteins implicated in
RT epileptic disorders.";
RL Trends Biochem. Sci. 27:441-444(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=19833108; DOI=10.1016/j.brainres.2009.10.013;
RA Herranz-Perez V., Olucha-Bordonau F.E., Morante-Redolat J.M., Perez-Tur J.;
RT "Regional distribution of the leucine-rich glioma inactivated (LGI) gene
RT family transcripts in the adult mouse brain.";
RL Brain Res. 1307:177-194(2010).
RN [4]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=30679375; DOI=10.1126/science.aau8977;
RA Favuzzi E., Deogracias R., Marques-Smith A., Maeso P., Jezequel J.,
RA Exposito-Alonso D., Balia M., Kroon T., Hinojosa A.J., Maraver E.F.,
RA Rico B.;
RT "Distinct molecular programs regulate synapse specificity in cortical
RT inhibitory circuits.";
RL Science 363:413-417(2019).
CC -!- FUNCTION: Required for the development of soma-targeting inhibitory
CC GABAergic synapses made by parvalbumin-positive basket cells.
CC {ECO:0000269|PubMed:30679375}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K4Z0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K4Z0-2; Sequence=VSP_007680, VSP_007681;
CC -!- TISSUE SPECIFICITY: Brain. {ECO:0000269|PubMed:19833108}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing parvalbumin-positive
CC basket cells. {ECO:0000269|PubMed:30679375}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC65852.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AJ487515; CAD31783.1; -; mRNA.
DR EMBL; AK122570; BAC65852.1; ALT_INIT; mRNA.
DR CCDS; CCDS19285.1; -. [Q8K4Z0-1]
DR CCDS; CCDS80281.1; -. [Q8K4Z0-2]
DR RefSeq; NP_001297533.1; NM_001310604.1. [Q8K4Z0-2]
DR RefSeq; NP_659194.1; NM_144945.3. [Q8K4Z0-1]
DR AlphaFoldDB; Q8K4Z0; -.
DR SMR; Q8K4Z0; -.
DR STRING; 10090.ENSMUSP00000040436; -.
DR GlyConnect; 2425; 2 N-Linked glycans (1 site). [Q8K4Z0-2]
DR GlyGen; Q8K4Z0; 4 sites.
DR PhosphoSitePlus; Q8K4Z0; -.
DR MaxQB; Q8K4Z0; -.
DR PaxDb; Q8K4Z0; -.
DR PeptideAtlas; Q8K4Z0; -.
DR PRIDE; Q8K4Z0; -.
DR ProteomicsDB; 265066; -. [Q8K4Z0-1]
DR ProteomicsDB; 265067; -. [Q8K4Z0-2]
DR Antibodypedia; 2543; 151 antibodies from 31 providers.
DR DNASU; 246316; -.
DR Ensembl; ENSMUST00000039750; ENSMUSP00000040436; ENSMUSG00000039252. [Q8K4Z0-1]
DR Ensembl; ENSMUST00000199942; ENSMUSP00000143707; ENSMUSG00000039252. [Q8K4Z0-2]
DR GeneID; 246316; -.
DR KEGG; mmu:246316; -.
DR UCSC; uc008xkm.1; mouse. [Q8K4Z0-1]
DR CTD; 55203; -.
DR MGI; MGI:2180196; Lgi2.
DR VEuPathDB; HostDB:ENSMUSG00000039252; -.
DR eggNOG; ENOG502QR53; Eukaryota.
DR GeneTree; ENSGT00940000157294; -.
DR InParanoid; Q8K4Z0; -.
DR OMA; QDIEVTK; -.
DR OrthoDB; 365688at2759; -.
DR PhylomeDB; Q8K4Z0; -.
DR TreeFam; TF333155; -.
DR Reactome; R-MMU-5682910; LGI-ADAM interactions.
DR BioGRID-ORCS; 246316; 0 hits in 57 CRISPR screens.
DR ChiTaRS; Lgi2; mouse.
DR PRO; PR:Q8K4Z0; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8K4Z0; protein.
DR Bgee; ENSMUSG00000039252; Expressed in conjunctival fornix and 202 other tissues.
DR ExpressionAtlas; Q8K4Z0; baseline and differential.
DR Genevisible; Q8K4Z0; MM.
DR GO; GO:0005615; C:extracellular space; HDA:BHF-UCL.
DR GO; GO:1904862; P:inhibitory synapse assembly; IMP:UniProtKB.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR000483; Cys-rich_flank_reg_C.
DR InterPro; IPR009039; EAR.
DR InterPro; IPR005492; EPTP.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF03736; EPTP; 6.
DR Pfam; PF13855; LRR_8; 1.
DR SMART; SM00369; LRR_TYP; 2.
DR SMART; SM00082; LRRCT; 1.
DR PROSITE; PS50912; EAR; 7.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Leucine-rich repeat;
KW Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..550
FT /note="Leucine-rich repeat LGI family member 2"
FT /id="PRO_0000017709"
FT DOMAIN 26..62
FT /note="LRRNT"
FT REPEAT 83..104
FT /note="LRR 1"
FT REPEAT 107..128
FT /note="LRR 2"
FT DOMAIN 140..190
FT /note="LRRCT"
FT REPEAT 224..266
FT /note="EAR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 270..312
FT /note="EAR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 316..363
FT /note="EAR 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 365..408
FT /note="EAR 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 412..455
FT /note="EAR 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 457..499
FT /note="EAR 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 503..545
FT /note="EAR 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 159
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 276
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 407
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 135
FT /note="L -> LSLANNHIKALPRDVFSDLDSLIEL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_007680"
FT VAR_SEQ 192..223
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_007681"
SQ SEQUENCE 550 AA; 63006 MW; 56974B9DE663CCD1 CRC64;
MALWRGGGAL GLLLLSAACL IPPSAQVRRL ARCPATCSCT KESIICVGSS WVPRIVPGDI
SSLSLVNGTF LEIKDRMFSH LPSLQLLLLN SNSFTVIRDD AFAGLFHLEY LFIEGNKIET
ISRNAFRGLR DLTHLDLRGN KFECDCKAKW LYLWLKMTNS TVSDVLCIGP PEYQEKKLNE
VTSFDYECTT TGPQTDEAKQ RGWQLELSLG FCELIFVFQH PLSDFVVHQT LPYQSVSVDT
FNSKNDVYVA IAQPSMENCM VLEWDHIEMN FRSYDNITGQ SIVGCKAILI DDQVFVVVAQ
LFGGSHIYKY DESWTKFVKF QDIEVSRISK PNDIELFEID DETFFIIADS SKAGLSTVYK
WNSKGFYSYQ SLHEWFRDTD AEFVDIDGKS HLILSSRSQV PIILQWNKSS KKFVPHGDIP
NMEDVLAVKS FRMQNTLYLS LTRFIGDSRV MRWNSKQFVE VQALPSRGAM TLQPFSFKDN
HYLALGSDYT FSQIYQWDKE KQQFKKFKEI YVQAPRSFTA VSTDRRDFFF ASSFKGKTKI
FEHIIVDLSL
