LGI3_MOUSE
ID LGI3_MOUSE Reviewed; 548 AA.
AC Q8K406;
DT 03-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Leucine-rich repeat LGI family member 3;
DE AltName: Full=Leubrin;
DE AltName: Full=Leucine-rich glioma-inactivated protein 3;
DE Flags: Precursor;
GN Name=Lgi3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Brain;
RA Yun H.-Y., Lee S.E.;
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Hippocampus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH STX1A.
RX PubMed=18760330; DOI=10.1016/j.neulet.2008.08.044;
RA Park W.-J., Lee S.E., Kwon N.S., Baek K.J., Kim D.-S., Yun H.-Y.;
RT "Leucine-rich glioma inactivated 3 associates with syntaxin 1.";
RL Neurosci. Lett. 444:240-244(2008).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=19833108; DOI=10.1016/j.brainres.2009.10.013;
RA Herranz-Perez V., Olucha-Bordonau F.E., Morante-Redolat J.M., Perez-Tur J.;
RT "Regional distribution of the leucine-rich glioma inactivated (LGI) gene
RT family transcripts in the adult mouse brain.";
RL Brain Res. 1307:177-194(2010).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May participate in the regulation of neuronal exocytosis.
CC {ECO:0000269|PubMed:18760330}.
CC -!- SUBUNIT: Interacts with STX1A. {ECO:0000269|PubMed:18760330}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Cytoplasmic vesicle,
CC secretory vesicle, synaptic vesicle {ECO:0000269|PubMed:18760330}.
CC Synapse, synaptosome {ECO:0000269|PubMed:18760330}. Note=Found in the
CC synaptosomal membrane fraction.
CC -!- TISSUE SPECIFICITY: Brain. {ECO:0000269|PubMed:19833108}.
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DR EMBL; AF515590; AAM55219.1; -; mRNA.
DR EMBL; AY174077; AAO19739.1; -; mRNA.
DR EMBL; AK049831; BAC33943.1; -; mRNA.
DR EMBL; BC055315; AAH55315.1; -; mRNA.
DR EMBL; BC061460; AAH61460.1; -; mRNA.
DR CCDS; CCDS27255.1; -.
DR RefSeq; NP_660254.1; NM_145219.4.
DR AlphaFoldDB; Q8K406; -.
DR SMR; Q8K406; -.
DR STRING; 10090.ENSMUSP00000046705; -.
DR GlyGen; Q8K406; 2 sites.
DR iPTMnet; Q8K406; -.
DR PhosphoSitePlus; Q8K406; -.
DR SwissPalm; Q8K406; -.
DR MaxQB; Q8K406; -.
DR PaxDb; Q8K406; -.
DR PRIDE; Q8K406; -.
DR ProteomicsDB; 286189; -.
DR Antibodypedia; 22532; 149 antibodies from 26 providers.
DR DNASU; 213469; -.
DR Ensembl; ENSMUST00000047331; ENSMUSP00000046705; ENSMUSG00000033595.
DR GeneID; 213469; -.
DR KEGG; mmu:213469; -.
DR UCSC; uc007uoh.2; mouse.
DR CTD; 203190; -.
DR MGI; MGI:2182619; Lgi3.
DR VEuPathDB; HostDB:ENSMUSG00000033595; -.
DR eggNOG; ENOG502QSAR; Eukaryota.
DR GeneTree; ENSGT00940000160296; -.
DR HOGENOM; CLU_036403_0_0_1; -.
DR InParanoid; Q8K406; -.
DR OMA; NSNTFTM; -.
DR OrthoDB; 365688at2759; -.
DR PhylomeDB; Q8K406; -.
DR TreeFam; TF333155; -.
DR Reactome; R-MMU-5682910; LGI-ADAM interactions.
DR BioGRID-ORCS; 213469; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q8K406; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8K406; protein.
DR Bgee; ENSMUSG00000033595; Expressed in primary visual cortex and 164 other tissues.
DR ExpressionAtlas; Q8K406; baseline and differential.
DR Genevisible; Q8K406; MM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0043005; C:neuron projection; IEA:UniProtKB-KW.
DR GO; GO:0008021; C:synaptic vesicle; IDA:UniProtKB.
DR GO; GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
DR GO; GO:0017157; P:regulation of exocytosis; IDA:UniProtKB.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR000483; Cys-rich_flank_reg_C.
DR InterPro; IPR009039; EAR.
DR InterPro; IPR005492; EPTP.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR011041; Quinoprot_gluc/sorb_DH.
DR Pfam; PF03736; EPTP; 7.
DR Pfam; PF13855; LRR_8; 1.
DR SMART; SM00369; LRR_TYP; 3.
DR SMART; SM00082; LRRCT; 1.
DR SUPFAM; SSF50952; SSF50952; 1.
DR PROSITE; PS50912; EAR; 7.
DR PROSITE; PS51450; LRR; 3.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Exocytosis; Glycoprotein; Leucine-rich repeat;
KW Reference proteome; Repeat; Secreted; Signal; Synapse; Synaptosome.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..548
FT /note="Leucine-rich repeat LGI family member 3"
FT /id="PRO_0000017711"
FT DOMAIN 31..64
FT /note="LRRNT"
FT REPEAT 89..110
FT /note="LRR 1"
FT REPEAT 113..134
FT /note="LRR 2"
FT REPEAT 137..158
FT /note="LRR 3"
FT DOMAIN 170..220
FT /note="LRRCT"
FT REPEAT 222..264
FT /note="EAR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 268..310
FT /note="EAR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 314..361
FT /note="EAR 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 363..406
FT /note="EAR 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 410..453
FT /note="EAR 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 455..497
FT /note="EAR 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT REPEAT 501..543
FT /note="EAR 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00075"
FT CARBOHYD 189
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 311
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 548 AA; 61818 MW; 2DB303936354B958 CRC64;
MAGLRARRGP GRRLLVLSTL GFCLMLQVSA KRPPKTPPCP PSCSCTRDTA FCVDSKSVPK
NLPSEVISLT LVNAAFSEIQ DGAFSHLPLL QFLLLNSNKF TLIGDNAFIG LSHLQYLFIE
NNDIWALSKF TFRGLKSLTH LSLANNNLQT LPRDIFRPLD ILSDLDLRGN ALNCDCKVKW
LVEWLAHTNT TVAPIYCASP PRFQEHKVQD LPLREFDCIT TDFVLYQTLS FPAVSAEPFL
YSSDLYLALA QPGASACTIL KWDYVERQLR DYDRIPAPSA VHCKPMVVDG QLYVVVAQLF
GGSYIYHWDP NTTRFTKLQD IDPQRVRKPN DLEAFRIDGD WFFAVADSSK AGATSLYRWH
QNGFYSHQAL HAWHRDTDLE FVDGEGKPRL IVSSSSQAPV IYQWSRSQKQ FVAQGEVTQV
PDAQAVKHFR AGRDSYLCLS RYIGDSKILR WEGTRFSEVQ ALPSRGSLAL QPFLVGGHRY
LALGSDFSFT QIYQWDEGRQ KFVRFQELAV QAPRAFCYMP AGDAQLLLAP SFKGQTLVYR
HVVVDLSA
