LGRB_BREPA
ID LGRB_BREPA Reviewed; 5162 AA.
AC Q70LM6;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Linear gramicidin synthase subunit B;
DE Includes:
DE RecName: Full=ATP-dependent D-leucine adenylase;
DE Short=D-LeuA;
DE AltName: Full=D-leucine activase;
DE Includes:
DE RecName: Full=Leucine racemase [ATP-hydrolyzing];
DE EC=5.1.1.-;
DE Includes:
DE RecName: Full=ATP-dependent alanine adenylase;
DE Short=AlaA;
DE AltName: Full=Alanine activase;
DE Includes:
DE RecName: Full=ATP-dependent D-valine adenylase;
DE Short=D-ValA;
DE AltName: Full=D-valine activase;
DE Includes:
DE RecName: Full=Valine racemase [ATP-hydrolyzing];
DE EC=5.1.1.-;
GN Name=lgrB;
OS Brevibacillus parabrevis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus.
OX NCBI_TaxID=54914;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 8185 / DSM 362 / JCM 20017 / NBRC 3331 / NCDO 717 / NCIMB 8598
RC / IAM 1031;
RX PubMed=14670971; DOI=10.1074/jbc.m309658200;
RA Kessler N., Schuhmann H., Morneweg S., Linne U., Marahiel M.A.;
RT "The linear pentadecapeptide gramicidin is assembled by four multimodular
RT nonribosomal peptide synthetases that comprise 16 modules with 57 catalytic
RT domains.";
RL J. Biol. Chem. 279:7413-7419(2004).
CC -!- FUNCTION: Activates the 3rd to 6th amino acids (Ala, D-Leu, Ala and D-
CC Val) in linear gramicidin and catalyzes the formation of the peptide
CC bond between them. This enzyme is also responsible for the
CC epimerization of the 4th (D-Leu) and the 6th (D-Val) amino acids.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000305};
CC Note=Binds 4 phosphopantetheines covalently. {ECO:0000305};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=2.2 mM for glycine;
CC KM=1.5 mM for alanine;
CC -!- SUBUNIT: Large multienzyme complex composed of 4 subunits; LgrA, LgrB,
CC LgrC and LgrD.
CC -!- DOMAIN: Four module-bearing peptide synthase with a C-terminal
CC epimerization domain. Each module incorporates one amino acid into the
CC peptide product and can be further subdivided into domains responsible
CC for substrate adenylation, thiolation, condensation (not for the
CC initiation module), and epimerization (optional).
CC -!- MISCELLANEOUS: Linear gramicidin is a pentadecapeptide antibiotic
CC produced during sporulation.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ566197; CAD92850.1; -; Genomic_DNA.
DR SMR; Q70LM6; -.
DR PRIDE; Q70LM6; -.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR Gene3D; 1.10.1200.10; -; 4.
DR Gene3D; 3.30.300.30; -; 4.
DR Gene3D; 3.30.559.10; -; 6.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR010060; NRPS_synth.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR Pfam; PF00501; AMP-binding; 4.
DR Pfam; PF13193; AMP-binding_C; 4.
DR Pfam; PF00668; Condensation; 6.
DR Pfam; PF00550; PP-binding; 4.
DR SMART; SM00823; PKS_PP; 4.
DR SUPFAM; SSF47336; SSF47336; 4.
DR TIGRFAMs; TIGR01733; AA-adenyl-dom; 4.
DR TIGRFAMs; TIGR01720; NRPS-para261; 2.
DR PROSITE; PS00455; AMP_BINDING; 4.
DR PROSITE; PS50075; CARRIER; 4.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 1: Evidence at protein level;
KW Antibiotic biosynthesis; Isomerase; Ligase; Multifunctional enzyme;
KW Phosphopantetheine; Phosphoprotein; Repeat.
FT CHAIN 1..5162
FT /note="Linear gramicidin synthase subunit B"
FT /id="PRO_0000193090"
FT DOMAIN 963..1038
FT /note="Carrier 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 2027..2101
FT /note="Carrier 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 3541..3616
FT /note="Carrier 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 4601..4675
FT /note="Carrier 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 998
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 2062
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 3576
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 4636
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 5162 AA; 577904 MW; 26C1F1EBDEA75BDB CRC64;
MSRKKVDNIY PLTPMQEGML FHSLLDEGSE SYFEQMRFTI KGLIDPAILE QSLNALIERH
DILRTVFLLE KVQKPRQIVL RERKTKVQVL DITHLSEGEQ AAYLEDFAQK DRQASFDLAK
DVLIRLTLVR TSADTHTLFW SHHHILLDGW CIPIVLNDFF QIYQQRKGGL PVELGPVYPY
STYISWLGEQ DAEEAKASWA EYISGYEPTS FIHKQGGKNS YRQAELVFAI EQGLTDSLNK
LAKQLHVTLN NLFRAIWGLM LQRQCNTEDV VFGSVVSGRP SHLPNVEQMV GLFINTVPIR
VQAGAEQTFS ELVKQVQQEA LSLAKYHYLS LADIQGNQQL IDHILLFQNY PMGQQFLTRL
NQYNEEFTLT HLSAFEQTNY DLNVMVTPSD VITIKYIYNA AVFSEEQLLH ISRQLTTIMT
QVTNAPDILL QKLEVVDPAE KQLQLHSFND TYRHYPTDKL IHQIFEERAE REPERIALVM
GEQVLTYREL NEKANQLAKL LRARGIGPES MVSLLTERSA EMMIAILAIF KAGGAYLPID
PSHPKERIEY ILQDSRSELL LVNHRFLGAV DFADRIIDLE AAEIYQGAAD NLECVSHANH
LAYVIYTSGS TGKPKGVMIE HASLLNIIFA LQELYPLLEN DAYLLKTTYT FDVSVAEIFG
WILGSGRLVI LDPGAEKEPA HIWETMVNHG VTHVNFVPSM LIPFVDYVRD QQQESPLRYI
FAAGEAMPSE LVGKVYEALP GVILENIYGP TESTIYATKY SLAKDSQDVL VPIGKPLANI
QTHIVNKHGQ LQPVGVPGEL CIAGASLARG YWNNEALTNE KFVPHPFAAG QRMYRTGDLA
RYRQDGNIEY LGRIDHQVKI RGYRIELDEI RAQLIQEASI RDAVVIARTD HNGQAYLCAY
FIADKQWTVN ALREALRQTL PDYMVPSHFI QMEEFPLTSS GKIDRKALPL PDGRVHTGNV
YLAPRNPVEE LVVRIWEEVL NVSQVGVHDN FFELGGHSLL ATQVLSRTAK LFHVRLPMRE
IFTHQTVAEL ARRIQALRHG AEADKHSPIQ PSALQRADEL PLSYAQQRLW FLDRLIPDSA
MYNIPVGFRL RGTVDELVLE RALNEIIQRH ESLRTTFVDV DGRALQVIHT DVHLSLGVTD
LRDKPAAAKD AEWKQMAEED AATPFRLDQW PLLRAMLIRL EEQESVLWLN VHHIISDGWS
MDVLVNELSE VYETLLKGEA LPLAALPIQY RDYAVWQREK SQDDVWKEQL RYWKNKLDGS
EPLLPLPTDR PRAVVQSYRG DHLSFYVPGE VGQKLRELGR QEGATLFMTL LAAFKSFLYR
YTHANDILIG TPVAGRNRQE IENLIGFFVN MLVLRTDLSD DPTFVELLRR VRETAFDAFA
NEDVPFEKLV DELQIERSLS YSPLFQVLFA VQGMSTGVRE GETLAIAPDE VTLNQTTKFD
LTLTMIEAAD NGLKGVFEYS TDLFDRTTIE RMAEHFGNLL QAIAADPGQK IVELPLLGGA
EQSRMLVEWN QTDVAYSLDL LVHERVARIA QELPEQFAVI GEQGALTYAQ LDAKANQLAH
ALLKRGIGSE DLVGICVERS SEMQIGQLAI LKAGAAYVPM DPAYPRERLA FMIKDAGMSL
VLTQERLLDA LPQEAAALLC LDRDWQEIAA ESTAAPAIKT NADQLAYVIY TSGSTGTPKG
VEIEHGSLLN LVNWHQRAYS VSAEDRASQI AGTAFDASVW ETWPYLTAGA TICQPREEIR
LSPEKLRDWL VETGITISFL PTPLAENLLP LPWPTGAALR YMLTGGDTLH QYPTADVPFT
LVNQYGPTEN TVVATAGAVP VLGERESAPT IGRPIDNVSV YVLDENRQPV PVGVVGELYI
GGKSLARGYR NRPDLTEASF VPNPFSPIEG ARMYRTGDLV RYAADGSIEF IGRADDQVSI
RGFRVELGEI ESALYAHPAV AESVVIVRED VTPGVKRLVA YAVLHEGEER QTSELRQSLK
EMLPDYMVPS AIVLMEALPL TPNGKVDRRA LPLPDVAQTE WEGSFVEPQS DVERKLAEIW
QEVLGVETIG VHDNFFELGG DSILTIQIVS RANQAGLQLT PKHLFDAQTL AELAASAVVL
EKAPEMQAEQ GIVTGELPLT PIQTWFFEQD VRHVHHWNQS VMLAVREELD MTALTQAFAA
LPRQHDALRL RFQQVNGTWQ AAHGEIADED VLLVADLSSV PEAEREARMR HITDELQASL
DIEKGPLHRA AYFQLGAEQR LFIVIHHLVV DGVSWRIILE DLQTAYEQVK AGQKIAWPQK
TTSFKSWAEE LTTYAEQSAV DEYWTGMDSE QACGLPVDHP QGKNTEGLAV QVKAKLSADE
TRALLQEVPA AYRTQINDVL LSALTRTITD WTNKRALYVS VEGHGREPIV DGVDVSRTVG
WFTSLYPVLL ETEPDLAWGD LLKSIKEQVR AIPDKGIGYG IHRYLSRDGQ TAEMLRAKPQ
PEISFNYLGQ FGQGQTTDAA LFQIIPNWSA SNVSEDETRL YKLDVMSMVA QDQLEMSWTF
SRDLYEPGTI EKLAHDYVQA LRAIIAHCRT EQAGGYTPSD FPLAELDQNS LDKFIGHNRL
IENVYTLTPL QEGMLFHSLY EQAGGDYVVQ LALKLEHVNV EAFSAAWQKV VERHAILRTS
FLWSGLEKPH QVVHAKVKTF VERLDWRHLT AAEQEAGLQT YLEQDRKRGF DLARPPLMRW
TLIRLDASTF QFVWSFHHML LDGWSTPIVF QDWQAFYAAA SHGKEASLPA IPPFSAYIAW
LKRQNLEEAQ QYWRDYLQGF GVPTPLGMGK SGGSAGQPKE YADHKLLLSE RATANLLAFA
RKHQLTLNTV VQGAWALILA RYAGEAEVVF GTTNLGRPTD LPDAEAMVGL FINTLPVRVL
FPEQTTVIDW LQSLQQAQSE MRQYEFTPLV DIQSWSEVPR GQSLFDSIFV FENYLSGTSV
DSESGMLLGE VKAVEQTSYP LTLVVAPGEE LMLKLIYETG RFEQPAMDKV LAQLSSVLEA
IMREPHEQLA DLSIITEAER HKLLVEWNAT DMPYERNLVM HQLFEAQVEA TPDAQALVVG
TERLTYAELN KRANQLAHYL RAQGVGPEVL VAVLMERTTE MIVALLGIIK AGGAYVPIDP
AYPQDRIGYT LDDSQAAIVL TQERLLPMLP EHTAQVICLD RDWACMAVQP EANVPNLAAP
TNLSYVIYTS GSTGLPKGVA IQHSSVIAFI FWAKTVFSAE EMSGVLASTS ICFDLSVYEI
FVTLSCGGKV ILADNALHLP SLPAAKEVTL INTVPSAAKE LVRMNAIPPS VRVVNLAGEP
LPNTLAQSLY ALGHVQKVFN LYGPSEDTTY STYVQVTKGA KTEPTIGRPL ANTQAYVLDA
KLQPVPLGLP GELYLGGDGL ARGYLKRPKM TAERFLPNPF HPDPDARMYS TGDLVRYLPD
GQLEYLGRID HQVKIRGYRI ELGELEAVLR SHPQIKEAVV VAKEDKLGEK RLVAYITTKD
GECGDRAVLT SWAKAKLPEF MVPSFFVWLD AMPLTPNGKI DRKQLPEPEW GQVASAAGYV
APRNQTEVLV ASIWADVLGI EQVGVHDNFF ELGGHSLLAT RVASRLRETF AKEVPIRAIF
ERPTVAELSE TLGAIGQNET EAQMLPVSRE AHLPLSFAQQ RLWFLDRLMP DSTLYNIPSA
VRLLGDLDIA AWEKSLQVLI QRHESLRTTF GDVDGEAVQV IHSRLDGKLN VIDLRGMPAD
EREAEAHRLA GLEAATPFDL SQGPLLRTTL IRLAEQECVF LFNLHHIIFD GWSIGIFLKE
MRALYEAFVR EEAPELAEIT VQYADYAVWQ RKWLEGEVLA EQLAYWKEKL SGAEPLLALP
TDQPRPAVQT HDGAMHTIKL SGELYAKLNK LSQEEGATLF MTLLAAFQVL LYRYSGQEDI
LVGSPVAGRN RQETEPLIGF FINTLVLRTD LSGEPTFREL LARVRETAFE AYAHQDLPFE
KLVDELELER SLSYSPLFQV MFVLQNFQLN LDEKAGIRVA DFEMDKHLVT SKYDLTLTMA
EKQNGLFATF EYNTALFHEA TMERLSQHFI QLLEAIVHMP DQGIARLPLL NQSERAQLLV
EWNDTTTAYP RNKRVDQLFR ETALLYPERL AVVAGNQTLT YAELERRANQ TANYLQQKGV
RPGALVGLCV KRSLEMLIGM LGILKAGGAY VPLDPDYPEE RLAYMMGDAG ITVLLTQEQL
MPGLPSGERT TIALDRDWPL IAKESEQAPD VDTTAESLAY VIYTSGSTGL PKGTLVVHRG
IVRLVKETDY VTITEQDVFL QASTVSFDAA TFEIWGSLLN GAKLVLLPPE LPSLAEIGQA
IQSHHVTTLW LTAGLFTLMV DHHKEYLSGV RQLLVGGDIV SVPHVKKALE IAGLTVINGY
GPTENTTFTC CNPVTVMPES AHTFPIGRPI KNTTAYVLDR HMQPVPIGVT GELYIGGDGL
AEGYLNRPDL TAERFVPNPF ATDQAARLYR TGDLVRYLPD GLIEFIGRLD NQVKIRGFRI
ELSEVEAVLA KHPAITASVV IVHENEAGMK QLVAYAVKDA EQELGTAELR QHFKAHVPDY
MVPAAFVMLD ALPLTPNGKV DRKALPAPVL ERSREEDAFA AATSHVEQTL ADIWCAVLRM
DRIGIHDNFF ELGGDSILSI QIVARANKAG IHLTPKQLFD QQTIAELAKV AGQSTKVDAE
QGNVTGEVPL LPIQTWFFEQ KQPTPHHWNQ SMLLQVNEPL EEECLSQAVA QLLAHHDALR
LRYTFADGQW KQTYADVDSE VPLQVEDLSM SPPAQQARKI EKLAQQAQAS LDLQNGPLLK
VVYFDLGYDR PGRLLMVIHH LAVDGVSWRI LIEDLQTAYG QAEKGNKIQL PPKTTSYKAW
AEKLHKYASS ERMLVDQDYW LKAADELSGH PLPVHDWAEN TEANGRMWTI HLEEEETDAL
LQKVPSRYRV QINDILLTAL ALAYGKWTGE SALLVNLEGH GREELFEDVD LSRTVGWFTS
MYPLLIQLEP NTSSEDALAR VKEKLQQIPH KGLGYGLLRY MAQDPELVEK LKAIPQAPLS
FNYLGQFHQA ADAKALLAYA EGERGANSGP DNRRTHLIDV VGAVTEGKLG LSFLYNGRLY
SESHIETFAR HYTDALQSLI QAEKQSYRAE DFEDADLSQS ALNKVLARLK NRKGNELHGG
SH
