ID   LGRE_BREPA              Reviewed;         263 AA.
AC   Q70LM8;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Linear gramicidin dehydrogenase LgrE;
DE            EC=1.1.-.-;
GN   Name=lgrE; Synonyms=lgrT;
OS   Brevibacillus parabrevis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus.
OX   NCBI_TaxID=54914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8185 / DSM 362 / JCM 20017 / NBRC 3331 / NCDO 717 / NCIMB 8598
RC   / IAM 1031;
RX   PubMed=14670971; DOI=10.1074/jbc.m309658200;
RA   Kessler N., Schuhmann H., Morneweg S., Linne U., Marahiel M.A.;
RT   "The linear pentadecapeptide gramicidin is assembled by four multimodular
RT   nonribosomal peptide synthetases that comprise 16 modules with 57 catalytic
RT   domains.";
RL   J. Biol. Chem. 279:7413-7419(2004).
RN   [2]
RP   CHARACTERIZATION.
RC   STRAIN=ATCC 8185 / DSM 362 / JCM 20017 / NBRC 3331 / NCDO 717 / NCIMB 8598
RC   / IAM 1031;
RX   PubMed=15938641; DOI=10.1021/bi050074t;
RA   Schracke N., Linne U., Mahlert C., Marahiel M.A.;
RT   "Synthesis of linear gramicidin requires the cooperation of two independent
RT   reductases.";
RL   Biochemistry 44:8507-8513(2005).
CC   -!- FUNCTION: In the final step of gramicidin biosynthesis, reduces the
CC       pentadecapeptide-aldehyde intermediate, that is released from the
CC       terminal module of the non-ribosomal peptide synthetase LgrD, to the
CC       final product ethanolamine-containing gramicidin.
CC   -!- MISCELLANEOUS: The reduction is strictly NADPH-dependent.
CC   -!- MISCELLANEOUS: Linear gramicidine is a pentadecapeptide antibiotic
CC       produced during sporulation.
CC   -!- SIMILARITY: Belongs to the thioesterase family. {ECO:0000305}.
CC   -!- CAUTION: Although this protein is said to have a reductase activity, it
CC       clearly belongs to the thioesterase (hydrolase) family. {ECO:0000305}.
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DR   EMBL; AJ566197; CAD92848.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q70LM8; -.
DR   SMR; Q70LM8; -.
DR   STRING; 54914.AV540_01985; -.
DR   ESTHER; bacbr-q70lm8; Thioesterase.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR012223; TEII.
DR   InterPro; IPR001031; Thioesterase.
DR   PANTHER; PTHR11487; PTHR11487; 1.
DR   Pfam; PF00975; Thioesterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; NADP; Oxidoreductase.
FT   CHAIN           1..263
FT                   /note="Linear gramicidin dehydrogenase LgrE"
FT                   /id="PRO_0000180363"
FT   ACT_SITE        96
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   263 AA;  30103 MW;  885DE9A45B041641 CRC64;
     MQKTHVSPSR WLLSPKMTAE AEVLLFSFHY AGGHAGIYRE WQKKLPVQIG VCPVQLPGRS
     NRFMEPYYTD LSVMIRELAE ALLPHLNRPF AFFGHSMGAL VSFELARYLR NQYGIKPRHM
     FASGRHAPHL PDPGEAIHHL PDAEFLKGLR TLNGTPKELF ENEENEEILQ MLLPMLRADF
     TICEQYQYQE EEPLGCGLTA IGGWQDPDIT VAHMEAWRKH TSASFQMHML QGDHFFLHSE
     QEQLLAIIES TLQSYLVGYR GIG