ID   LGSN_RAT                Reviewed;         561 AA.
AC   Q7TT51;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Lengsin;
DE   AltName: Full=Glutamate-ammonia ligase domain-containing protein 1;
DE   AltName: Full=Lens glutamine synthase-like;
GN   Name=Lgsn; Synonyms=Gluld1, Lgs;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Eye;
RA   Wistow G.;
RL   Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May act as a component of the cytoskeleton or as a chaperone
CC       for the reorganization of intermediate filament proteins during
CC       terminal differentiation in the lens. Does not seem to have enzymatic
CC       activity (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Dodecamer. Interacts with BFSP2 AND VIM. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in lens.
CC   -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
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DR   EMBL; AY280501; AAP34385.1; -; mRNA.
DR   RefSeq; NP_852048.1; NM_181383.2.
DR   RefSeq; XP_017451926.1; XM_017596437.1.
DR   AlphaFoldDB; Q7TT51; -.
DR   SMR; Q7TT51; -.
DR   STRING; 10116.ENSRNOP00000016316; -.
DR   PaxDb; Q7TT51; -.
DR   PRIDE; Q7TT51; -.
DR   Ensembl; ENSRNOT00000016316; ENSRNOP00000016316; ENSRNOG00000012205.
DR   GeneID; 316304; -.
DR   KEGG; rno:316304; -.
DR   UCSC; RGD:727925; rat.
DR   CTD; 51557; -.
DR   RGD; 727925; Lgsn.
DR   eggNOG; KOG0683; Eukaryota.
DR   GeneTree; ENSGT00390000013639; -.
DR   HOGENOM; CLU_017290_0_2_1; -.
DR   InParanoid; Q7TT51; -.
DR   OMA; TFYTHPE; -.
DR   OrthoDB; 1424442at2759; -.
DR   PhylomeDB; Q7TT51; -.
DR   TreeFam; TF331605; -.
DR   PRO; PR:Q7TT51; -.
DR   Proteomes; UP000002494; Chromosome 9.
DR   Bgee; ENSRNOG00000012205; Expressed in liver and 1 other tissue.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 3.10.20.70; -; 1.
DR   InterPro; IPR036651; Gln_synt_N.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR008146; Gln_synth_cat_dom.
DR   Pfam; PF00120; Gln-synt_C; 1.
DR   SMART; SM01230; Gln-synt_C; 1.
DR   SUPFAM; SSF54368; SSF54368; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   PROSITE; PS51986; GS_BETA_GRASP; 1.
DR   PROSITE; PS51987; GS_CATALYTIC; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome.
FT   CHAIN           1..561
FT                   /note="Lengsin"
FT                   /id="PRO_0000153284"
FT   DOMAIN          135..229
FT                   /note="GS beta-grasp"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT   DOMAIN          236..561
FT                   /note="GS catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
FT   REGION          1..78
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          91..112
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        7..26
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        52..78
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   561 AA;  62269 MW;  F3BE12C50751DDEC CRC64;
     MNDEGDLAQE DTTKDEANST EGSRVNKLKR TRRKVTKPHL CSADGDEITM ANSREMSRNQ
     TADLSKPGSA ESWSWHNAKD AQDQIPVVKS SLPSAGAPDA EFNPNTDHTR DNAQSLILPQ
     LSSRMKHIKQ EMAKNHLQFV RFEATDLHGV SRSKSIPAQF FQEKVIHGVF MPRGYLELMP
     NPKDNEVNHI RATCFNSDIV LMPELSTFRV LPWAERTARV ICDTFTVTGE PLLTSPRYIA
     KRQLRQLQDA GFSLLSAFIY DFCIFGVPEV INSKTISFPA STLLSNHDQP FMQELVDGLY
     HTGANVESFS SSTRPGQMEI CFLPEFGISS ADNAFTLRTG VQEVARRYNY IASLVIETGF
     CNSGILSHSI WDVSGKTNMF YSGSGVERLT LTGKKWLAGL LKHSAALSCL MAPAVNCRKR
     YCKDSRDLKD SVPTTWGYND NSCALNVKCH GEKGTQIENK LGSATANPYL VLAATVAAGL
     DGLQSSDGAA AESDESQDLY QPEPSEIPLK MEDALAALEQ DECLKQALGE TFIRYFVAMK
     KYELENEETD AEGNKFLEYF I