ID   LGTB_NEIMB              Reviewed;         275 AA.
AC   Q51116;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Lacto-N-neotetraose biosynthesis glycosyltransferase LgtB;
DE            EC=2.-.-.-;
GN   Name=lgtB; OrderedLocusNames=NMB1928;
OS   Neisseria meningitidis serogroup B (strain MC58).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=122586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=8817494; DOI=10.1111/j.1365-2958.1995.mmi_18040729.x;
RA   Jennings M.P., Hood D., Peak I.R.A., Virji M., Moxon E.R.;
RT   "Molecular analysis of a locus for the biosynthesis and phase-variable
RT   expression of the lacto-N-neotetraose terminal lipopolysaccharide structure
RT   in Neisseria meningitidis.";
RL   Mol. Microbiol. 18:729-740(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA   Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA   Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA   Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA   Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA   Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA   Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA   Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA   Moxon E.R., Rappuoli R., Venter J.C.;
RT   "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT   MC58.";
RL   Science 287:1809-1815(2000).
CC   -!- FUNCTION: Adds the second galactose to the lacto-N-tetraose chain in
CC       lipooligosaccharide (LOS).
CC   -!- PATHWAY: Glycan metabolism; lacto-N-neotetraose biosynthesis.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipooligosaccharide
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 25 family.
CC       {ECO:0000305}.
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DR   EMBL; U25839; AAC44085.1; -; Genomic_DNA.
DR   EMBL; AE002098; AAF42257.1; -; Genomic_DNA.
DR   PIR; C81027; C81027.
DR   PIR; S70814; S70814.
DR   RefSeq; NP_274922.1; NC_003112.2.
DR   RefSeq; WP_002225824.1; NC_003112.2.
DR   AlphaFoldDB; Q51116; -.
DR   STRING; 122586.NMB1928; -.
DR   CAZy; GT25; Glycosyltransferase Family 25.
DR   PaxDb; Q51116; -.
DR   EnsemblBacteria; AAF42257; AAF42257; NMB1928.
DR   KEGG; nme:NMB1928; -.
DR   PATRIC; fig|122586.8.peg.2456; -.
DR   HOGENOM; CLU_071269_2_1_4; -.
DR   OMA; WRDSPAN; -.
DR   BRENDA; 2.4.1.38; 16309.
DR   UniPathway; UPA00501; -.
DR   UniPathway; UPA00820; -.
DR   Proteomes; UP000000425; Chromosome.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd06532; Glyco_transf_25; 1.
DR   InterPro; IPR002654; Glyco_trans_25.
DR   Pfam; PF01755; Glyco_transf_25; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipopolysaccharide biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..275
FT                   /note="Lacto-N-neotetraose biosynthesis glycosyltransferase
FT                   LgtB"
FT                   /id="PRO_0000216237"
FT   CONFLICT        97
FT                   /note="A -> E (in Ref. 1; AAC44085)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   275 AA;  31578 MW;  E871305E2F6CF70F CRC64;
     MQNHVISLAS AAERRAHIAD TFGRHGIPFQ FFDALMPSER LEQAMAELVP GLSAHPYLSG
     VEKACFMSHA VLWKQALDEG LPYITVFEDD VLLGEGAEKF LAEDAWLQER FDPDTAFIVR
     LETMFMHVLT SPSGVADYCG RAFPLLESEH WGTAGYIISR KAMRFFLDRF AALPPEGLHP
     VDLMMFSDFF DREGMPVCQL NPALCAQELH YAKFHDQNSA LGSLIEHDRL LNRKQQRRDS
     PANTFKHRLI RALTKISRER EKRRQRREQF IVPFQ