ID   LGTE_NEIGO              Reviewed;         280 AA.
AC   Q50950;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Lacto-N-neotetraose biosynthesis glycosyltransferase LgtE;
DE            EC=2.-.-.-;
GN   Name=lgtE;
OS   Neisseria gonorrhoeae.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=485;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33084 / F62 / M-1914;
RX   PubMed=7964493; DOI=10.1084/jem.180.6.2181;
RA   Gotschlich E.C.;
RT   "Genetic locus for the biosynthesis of the variable portion of Neisseria
RT   gonorrhoeae lipooligosaccharide.";
RL   J. Exp. Med. 180:2181-2190(1994).
CC   -!- FUNCTION: Adds the first galactose to the lacto-N-tetraose chain in
CC       lipooligosaccharide (LOS).
CC   -!- PATHWAY: Glycan metabolism; lacto-N-neotetraose biosynthesis.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipooligosaccharide
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 25 family.
CC       {ECO:0000305}.
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DR   EMBL; U14554; AAA68013.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q50950; -.
DR   CAZy; GT25; Glycosyltransferase Family 25.
DR   UniPathway; UPA00501; -.
DR   UniPathway; UPA00820; -.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd06532; Glyco_transf_25; 1.
DR   InterPro; IPR002654; Glyco_trans_25.
DR   Pfam; PF01755; Glyco_transf_25; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipopolysaccharide biosynthesis; Transferase.
FT   CHAIN           1..280
FT                   /note="Lacto-N-neotetraose biosynthesis glycosyltransferase
FT                   LgtE"
FT                   /id="PRO_0000216238"
SQ   SEQUENCE   280 AA;  32420 MW;  F043597BA3040407 CRC64;
     MQNHVISLAS AAERRAHIAD TFGSRGIPFQ FFDALMPSER LEQAMAELVP GLSAHPYLSG
     VEKACFMSHA VLWEQALDEG LPYIAVFEDD VLLGEGAEQF LAEDTWLEER FDKDSAFIVR
     LETMFAKVIV RPDKVLNYEN RSFPLLESEH CGTAGYIISR EAMRFFLDRF AVLPPERIKA
     VDLMMFTYFF DKEGMPVYQV SPALCTQELH YAKFLSQNSM LGSDLEKDRE QGRRHRRSLK
     VMFDLKRALG KFGREKKKRM ERQRQAELEK VYGRRVILFK