ID   LGTE_NEIMB              Reviewed;         280 AA.
AC   Q51117;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Lacto-N-neotetraose biosynthesis glycosyltransferase LgtE;
DE            EC=2.-.-.-;
GN   Name=lgtE; OrderedLocusNames=NMB1926;
OS   Neisseria meningitidis serogroup B (strain MC58).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=122586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=8817494; DOI=10.1111/j.1365-2958.1995.mmi_18040729.x;
RA   Jennings M.P., Hood D., Peak I.R.A., Virji M., Moxon E.R.;
RT   "Molecular analysis of a locus for the biosynthesis and phase-variable
RT   expression of the lacto-N-neotetraose terminal lipopolysaccharide structure
RT   in Neisseria meningitidis.";
RL   Mol. Microbiol. 18:729-740(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA   Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA   Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA   Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA   Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA   Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA   Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA   Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA   Moxon E.R., Rappuoli R., Venter J.C.;
RT   "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT   MC58.";
RL   Science 287:1809-1815(2000).
CC   -!- FUNCTION: Adds the first galactose to the lacto-N-tetraose chain in
CC       lipooligosaccharide (LOS).
CC   -!- PATHWAY: Glycan metabolism; lacto-N-neotetraose biosynthesis.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipooligosaccharide
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 25 family.
CC       {ECO:0000305}.
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DR   EMBL; U25839; AAC44086.1; -; Genomic_DNA.
DR   EMBL; AE002098; AAF42255.1; -; Genomic_DNA.
DR   PIR; A81027; A81027.
DR   PIR; S70815; S70815.
DR   RefSeq; NP_274920.1; NC_003112.2.
DR   RefSeq; WP_002225823.1; NC_003112.2.
DR   AlphaFoldDB; Q51117; -.
DR   STRING; 122586.NMB1926; -.
DR   CAZy; GT25; Glycosyltransferase Family 25.
DR   PaxDb; Q51117; -.
DR   EnsemblBacteria; AAF42255; AAF42255; NMB1926.
DR   KEGG; nme:NMB1926; -.
DR   PATRIC; fig|122586.8.peg.2455; -.
DR   HOGENOM; CLU_071269_2_0_4; -.
DR   OMA; DLMMFTY; -.
DR   UniPathway; UPA00501; -.
DR   UniPathway; UPA00820; -.
DR   Proteomes; UP000000425; Chromosome.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd06532; Glyco_transf_25; 1.
DR   InterPro; IPR002654; Glyco_trans_25.
DR   Pfam; PF01755; Glyco_transf_25; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipopolysaccharide biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..280
FT                   /note="Lacto-N-neotetraose biosynthesis glycosyltransferase
FT                   LgtE"
FT                   /id="PRO_0000216239"
FT   CONFLICT        16
FT                   /note="A -> G (in Ref. 1; AAC44086)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        89..92
FT                   /note="Missing (in Ref. 1; AAC44086)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        176..178
FT                   /note="EWI -> RVD (in Ref. 1; AAC44086)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   280 AA;  32790 MW;  826827E942BA5842 CRC64;
     MQNHVISLAS AAERRAHIAA TFGVRGIPFQ FFDALMPSEE LNRMMAELVP GLAKQHLLSE
     VEKACFMSHA VLWKQALDEG LPYVAVFEDD VLLGKDAEKF LAEDTWLEER FDKDSAFIVR
     LETMFAKVIV RPDKVLNYEN RSFPLLESEH WGTAGYIISR EAMRFFLERF AVLPAEWIKA
     VDWMMFTYFF DKEGMPVYQV NPALCTQELH YAKFLSKNSM LGSDLEKDRE QERRHRRSLK
     VMFDLKRALG KFGREKKKRM ERQRQAELEK AYGRRVISFK