ID   LGT_CITUN               Reviewed;         511 AA.
AC   Q9MB73;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Limonoid UDP-glucosyltransferase;
DE            Short=LGTase;
DE            Short=Limonoid GTase;
DE            Short=Limonoid glucosyltransferase;
DE            EC=2.4.1.210;
OS   Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX   NCBI_TaxID=55188;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Miyagawa-Wase; TISSUE=Albedo;
RX   PubMed=10713266; DOI=10.1016/s0014-5793(00)01275-8;
RA   Kita M., Hirata Y., Moriguchi T., Endo-Inagaki T., Matsumoto R.,
RA   Hasegawa S., Suhayda C.G., Omura M.;
RT   "Molecular cloning and characterization of a novel gene encoding limonoid
RT   UDP-glucosyltransferase in Citrus.";
RL   FEBS Lett. 469:173-178(2000).
CC   -!- FUNCTION: Involved in the glucosylation of limonoids.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + limonin + UDP-alpha-D-glucose = glucosyl-limonin + 2
CC         H(+) + UDP; Xref=Rhea:RHEA:11256, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16226, ChEBI:CHEBI:57626,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.210;
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB033758; BAA93039.1; -; mRNA.
DR   AlphaFoldDB; Q9MB73; -.
DR   SMR; Q9MB73; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   BRENDA; 2.4.1.210; 1428.
DR   GO; GO:0050645; F:limonoid glucosyltransferase activity; IEA:UniProtKB-EC.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   2: Evidence at transcript level;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..511
FT                   /note="Limonoid UDP-glucosyltransferase"
FT                   /id="PRO_0000074171"
SQ   SEQUENCE   511 AA;  57478 MW;  05D91AB69BCA1CCD CRC64;
     MGTESLVHVL LVSFPGHGHV NPLLRLGRLL ASKGFFLTLT TPESFGKQMR KAGNFTYEPT
     PVGDGFIRFE FFEDGWDEDD PRREDLDQYM AQLELIGKQV IPKIIKKSAE EYRPVSCLIN
     NPFIPWVSDV AESLGLPSAM LWVQSCACFA AYYHYFHGLV PFPSEKEPEI DVQLPCMPLL
     KHDEMPSFLH PSTPYPFLRR AILGQYENLG KPFCILLDTF YELEKEIIDY MAKICPIKPV
     GPLFKNPKAP TLTVRDDCMK PDECIDWLDK KPPSSVVYIS FGTVVYLKQE QVEEIGYALL
     NSGISFLWVM KPPPEDSGVK IVDLPDGFLE KVGDKGKVVQ WSPQEKVLAH PSVACFVTHC
     GWNSTMESLA SGVPVITFPQ WGDQVTDAMY LCDVFKTGLR LCRGEAENRI ISRDEVEKCL
     LEATAGPKAV ALEENALKWK KEAEEAVADG GSSDRNIQAF VDEVRRTSVE IITSSKSKSI
     HRVKELVEKT ATATANDKVE LVESRRTRVQ Y