LHPL4_RAT
ID LHPL4_RAT Reviewed; 247 AA.
AC Q7TSY2;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=LHFPL tetraspan subfamily member 4 protein {ECO:0000312|RGD:727967};
DE AltName: Full=Lipoma HMGIC fusion partner-like 4 protein {ECO:0000250|UniProtKB:Q7Z7J7};
GN Name=Lhfpl4 {ECO:0000312|RGD:727967};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Huang C.Q., Wu S.L., Liu S.;
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=28978485; DOI=10.1016/j.celrep.2017.09.025;
RA Davenport E.C., Pendolino V., Kontou G., McGee T.P., Sheehan D.F.,
RA Lopez-Domenech G., Farrant M., Kittler J.T.;
RT "An essential role for the tetraspanin LHFPL4 in the cell-type-specific
RT targeting and clustering of synaptic GABAA ceceptors.";
RL Cell Rep. 21:70-83(2017).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH
RP NLGN2; GABRA1; GABRB2; GABRG2 AND GABRB3, AND INTERACTION WITH GABRA1.
RX PubMed=28279354; DOI=10.1016/j.neuron.2017.02.023;
RA Yamasaki T., Hoyos-Ramirez E., Martenson J.S., Morimoto-Tomita M.,
RA Tomita S.;
RT "GARLH family proteins stabilize GABAA receptors at synapses.";
RL Neuron 93:1138-1152(2017).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=29742426; DOI=10.1016/j.celrep.2018.04.015;
RA Wu M., Tian H.L., Liu X., Lai J.H.C., Du S., Xia J.;
RT "Impairment of inhibitory synapse formation and motor behavior in mice
RT lacking the NL2 binding partner LHFPL4/GARLH4.";
RL Cell Rep. 23:1691-1705(2018).
CC -!- FUNCTION: Plays a role in the regulation of inhibitory synapse
CC formation and function by being involved in maintening gamma-
CC aminobutyric acid receptors (GABAARs) clustering and their associated
CC scaffold proteins at inhibitory synaptic sites. Acts in concert with
CC NLGN2 to recruit or stabilize GABAARs. {ECO:0000250|UniProtKB:Q5U4E0}.
CC -!- SUBUNIT: Interacts with GABA(A) receptor subunits (PubMed:28279354).
CC Interacts with GABRB3 (By similarity). Interacts with GABRA2 (By
CC similarity). Interacts with GABRG2 (By similarity). Interacts with
CC GABRA1 (PubMed:28279354). Identified in a complex of 720 kDa composed
CC of LHFPL4, NLGN2, GABRA1, GABRB2, GABRG2 and GABRB3 (PubMed:28279354).
CC Interacts with NLGN2; leading to mutual regulation of protein level and
CC synaptic clustering (By similarity). {ECO:0000250|UniProtKB:Q5U4E0,
CC ECO:0000269|PubMed:28279354}.
CC -!- SUBCELLULAR LOCATION: Cell projection, dendrite
CC {ECO:0000269|PubMed:28978485}. Postsynaptic cell membrane
CC {ECO:0000269|PubMed:28978485, ECO:0000269|PubMed:29742426}; Multi-pass
CC membrane protein {ECO:0000255}. Note=Specifically localizes to
CC inhibitory postsynaptic sites (PubMed:29742426). Colocalizes with GPHN,
CC GABRG2 and NLGN2 at inhibitory postsynaptic sites (PubMed:29742426,
CC PubMed:28978485). {ECO:0000269|PubMed:28978485,
CC ECO:0000269|PubMed:29742426}.
CC -!- SIMILARITY: Belongs to the LHFP family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY278321; AAP37014.1; -; mRNA.
DR RefSeq; NP_852052.1; NM_181387.2.
DR AlphaFoldDB; Q7TSY2; -.
DR SMR; Q7TSY2; -.
DR STRING; 10116.ENSRNOP00000010347; -.
DR PaxDb; Q7TSY2; -.
DR GeneID; 353230; -.
DR UCSC; RGD:727967; rat.
DR CTD; 375323; -.
DR RGD; 727967; Lhfpl4.
DR eggNOG; KOG4026; Eukaryota.
DR HOGENOM; CLU_084868_1_2_1; -.
DR InParanoid; Q7TSY2; -.
DR OMA; SHMYATN; -.
DR OrthoDB; 1219117at2759; -.
DR PhylomeDB; Q7TSY2; -.
DR TreeFam; TF321143; -.
DR PRO; PR:Q7TSY2; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000007727; Expressed in frontal cortex and 3 other tissues.
DR Genevisible; Q7TSY2; RN.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
DR GO; GO:0060077; C:inhibitory synapse; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; ISS:UniProtKB.
DR GO; GO:0050811; F:GABA receptor binding; IDA:UniProtKB.
DR GO; GO:0097112; P:gamma-aminobutyric acid receptor clustering; ISS:UniProtKB.
DR GO; GO:1905702; P:regulation of inhibitory synapse assembly; ISS:UniProtKB.
DR GO; GO:0007605; P:sensory perception of sound; IBA:GO_Central.
DR InterPro; IPR019372; LHFPL.
DR PANTHER; PTHR12489; PTHR12489; 1.
DR Pfam; PF10242; L_HMGIC_fpl; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cell projection; Membrane; Postsynaptic cell membrane;
KW Reference proteome; Synapse; Transmembrane; Transmembrane helix.
FT CHAIN 1..247
FT /note="LHFPL tetraspan subfamily member 4 protein"
FT /id="PRO_0000285963"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 247 AA; 27050 MW; CB1F73E45A0E32BD CRC64;
MLPSQEASKL YHEHYMRNSR AIGVLWAIFT ICFAIINVVV FIQPYWVGDS VSTPKPGYFG
LFHYCVGSGL AGRELTCRGS FTDFSTIPSS AFKAAAFFVL LSMVLILGCI TCFALFFFCN
TATVYKICAW MQLLAALCLV LGCMIFPDGW DAETIRDMCG AKTGKYSLGD CSVRWAYILA
IIGILNALIL SFLAFVLGNR QTDLLQEELK QENKDFVGTT VSSVLRPGGD VSGWGVLPCP
VAHTQGP
