LHT2_ARATH
ID LHT2_ARATH Reviewed; 441 AA.
AC Q9LRB5;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Lysine histidine transporter 2;
DE Short=AtLHT2;
DE AltName: Full=Amino acid transporter-like protein 2;
GN Name=LHT2; Synonyms=AATL2; OrderedLocusNames=At1g24400; ORFNames=F21J9.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Uefuji H., Takase H., Hiratsuka K.;
RT "Arabidopsis AATL2 mRNA encoding putative amino acid transporter.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP TISSUE SPECIFICITY.
RC STRAIN=cv. C24;
RX PubMed=15361141; DOI=10.1111/j.1365-313x.2004.02186.x;
RA Lee Y.-H., Tegeder M.;
RT "Selective expression of a novel high-affinity transport system for acidic
RT and neutral amino acids in the tapetum cells of Arabidopsis flowers.";
RL Plant J. 40:60-74(2004).
CC -!- FUNCTION: Amino acid-proton symporter. Transporter with a broad
CC specificity for neutral and acidic amino acids. Basic amino acids are
CC only marginally transported. Involved in import of amino acids into the
CC tapetum cells for synthesis of compounds important for microspore
CC structure. {ECO:0000269|PubMed:15361141}.
CC -!- ACTIVITY REGULATION: Inhibited by diethylstibestrol (DES), 2,4-
CC dinitrophenol (DNP) and carbonlycyanide m-chlorophenylhydrazone (CCCP).
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=72 uM for aspartate {ECO:0000269|PubMed:15361141};
CC KM=13 uM for proline {ECO:0000269|PubMed:15361141};
CC pH dependence:
CC Optimum pH is 4-5. {ECO:0000269|PubMed:15361141};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in flower buds and to lower levels in
CC leaves and stems. Not detected in roots and siliques. Restricted to the
CC tapetum cell layer. {ECO:0000269|PubMed:15361141}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.
CC {ECO:0000305}.
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DR EMBL; AB046210; BAB01766.1; -; mRNA.
DR EMBL; AC000103; AAF97980.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30526.1; -; Genomic_DNA.
DR PIR; C86378; C86378.
DR RefSeq; NP_564217.1; NM_102286.3.
DR AlphaFoldDB; Q9LRB5; -.
DR SMR; Q9LRB5; -.
DR BioGRID; 24295; 39.
DR IntAct; Q9LRB5; 39.
DR STRING; 3702.AT1G24400.1; -.
DR TCDB; 2.A.18.2.9; the amino acid/auxin permease (aaap) family.
DR PaxDb; Q9LRB5; -.
DR PRIDE; Q9LRB5; -.
DR ProteomicsDB; 238468; -.
DR EnsemblPlants; AT1G24400.1; AT1G24400.1; AT1G24400.
DR GeneID; 839057; -.
DR Gramene; AT1G24400.1; AT1G24400.1; AT1G24400.
DR KEGG; ath:AT1G24400; -.
DR Araport; AT1G24400; -.
DR TAIR; locus:2024071; AT1G24400.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_031160_0_0_1; -.
DR InParanoid; Q9LRB5; -.
DR OMA; MWFFLLK; -.
DR OrthoDB; 570025at2759; -.
DR PhylomeDB; Q9LRB5; -.
DR PRO; PR:Q9LRB5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LRB5; baseline and differential.
DR Genevisible; Q9LRB5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015172; F:acidic amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..441
FT /note="Lysine histidine transporter 2"
FT /id="PRO_0000387970"
FT TOPO_DOM 1..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..58
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..110
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 132..152
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 174..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..222
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..263
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..300
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..347
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 371..373
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 397..406
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 428..441
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT VARIANT 361
FT /note="V -> I (in strain: cv. C24)"
SQ SEQUENCE 441 AA; 49152 MW; C8ED338517F4B981 CRC64;
MGNSEMSASE VAAAKQKNVD DWLPITSSRN AKWWYSAFHN VTAMVGAGVL SLPYAMSNLG
WGPGVTIMVM SWIITLYTLW QMVEMHEIVP GKRLDRYHEL GQHAFGEKLG LWIVVPQQLI
VEVGVDIVYM VTGGASLKKV HQLVCPDCKE IRTTFWIMIF ASVHFVISHL PNFNSISIIS
LAAAVMSLTY STIAWAASVH KGVHPDVDYS PRASTDVGKV FNFLNALGDV AFAYAGHNVV
LEIQATIPST PEMPSKVPMW RGVIVAYIVV AICYFPVAFL GYYIFGNSVD DNILITLEKP
IWLIAMANMF VVIHVIGSYQ IFAMPVFDML ETVLVKKMNF NPSFKLRFIT RSLYVAFTMI
VAICVPFFGG LLGFFGGFAF APTTYYLPCI MWLVLKKPKR FGLSWTANWF CIIVGVLLTI
LAPIGGLRTI IINAKTYKFF S
