LHTL8_ARATH
ID LHTL8_ARATH Reviewed; 519 AA.
AC Q9SX98; Q9SXF7;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Lysine histidine transporter-like 8;
DE AltName: Full=Amino acid transporter-like protein 1;
GN Name=AATL1; OrderedLocusNames=At1g47670; ORFNames=F16N3.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Uefuji H., Takase H., Hiratsuka K.;
RT "Arabidopsis AATL1 gene encoding putative amino acid transporter.";
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. La-0;
RX PubMed=14506206; DOI=10.1074/mcp.t300006-mcp200;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Large-scale analysis of in vivo phosphorylated membrane proteins by
RT immobilized metal ion affinity chromatography and mass spectrometry.";
RL Mol. Cell. Proteomics 2:1234-1243(2003).
RN [6]
RP SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RX PubMed=15308754; DOI=10.1105/tpc.104.023150;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Phosphoproteomics of the Arabidopsis plasma membrane and a new
RT phosphorylation site database.";
RL Plant Cell 16:2394-2405(2004).
RN [7]
RP GENE FAMILY.
RC STRAIN=cv. C24;
RX PubMed=15361141; DOI=10.1111/j.1365-313x.2004.02186.x;
RA Lee Y.-H., Tegeder M.;
RT "Selective expression of a novel high-affinity transport system for acidic
RT and neutral amino acids in the tapetum cells of Arabidopsis flowers.";
RL Plant J. 40:60-74(2004).
CC -!- FUNCTION: Amino acid transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15308754};
CC Multi-pass membrane protein {ECO:0000269|PubMed:15308754}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.
CC {ECO:0000305}.
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DR EMBL; AB030586; BAA82706.1; -; Genomic_DNA.
DR EMBL; AC007519; AAD46019.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32199.1; -; Genomic_DNA.
DR EMBL; AY065445; AAL38886.1; -; mRNA.
DR EMBL; AY117276; AAM51351.1; -; mRNA.
DR PIR; F96517; F96517.
DR RefSeq; NP_175198.1; NM_103660.6.
DR AlphaFoldDB; Q9SX98; -.
DR BioGRID; 26402; 31.
DR IntAct; Q9SX98; 30.
DR STRING; 3702.AT1G47670.1; -.
DR TCDB; 2.A.18.2.8; the amino acid/auxin permease (aaap) family.
DR iPTMnet; Q9SX98; -.
DR PaxDb; Q9SX98; -.
DR PRIDE; Q9SX98; -.
DR ProteomicsDB; 238380; -.
DR EnsemblPlants; AT1G47670.1; AT1G47670.1; AT1G47670.
DR GeneID; 841177; -.
DR Gramene; AT1G47670.1; AT1G47670.1; AT1G47670.
DR KEGG; ath:AT1G47670; -.
DR Araport; AT1G47670; -.
DR TAIR; locus:2015383; AT1G47670.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_031160_2_1_1; -.
DR InParanoid; Q9SX98; -.
DR OMA; IVCGPIC; -.
DR OrthoDB; 570025at2759; -.
DR PhylomeDB; Q9SX98; -.
DR PRO; PR:Q9SX98; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SX98; baseline and differential.
DR Genevisible; Q9SX98; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..519
FT /note="Lysine histidine transporter-like 8"
FT /id="PRO_0000387978"
FT TOPO_DOM 1..114
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..176
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..217
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 239..243
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 265..282
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..333
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 355..377
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..427
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 428..448
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 449
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 471..485
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 486..506
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 507..519
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 240
FT /note="N -> D (in Ref. 1; BAA82706)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 519 AA; 57119 MW; DC578D98AC2C813D CRC64;
MDERPETELI SIPATPRVST PEILTPSGQR SPRPATKPSS ATWTPTSFIS PRFLSPIGTP
MKRVLVNMKG YLEEVGHLTK LNPQDAWLPI TESRNGNAHY AAFHNLNAGV GFQALVLPVA
FAFLGWSWGI LSLTIAYCWQ LYTLWILVQL HEAVPGKRYN RYVELAQAAF GERLGVWLAL
FPTVYLSAGT ATALILIGGE TMKLFFQIVC GPLCTSNPLT TVEWYLVFTS LCIVLSQLPN
LNSIAGLSLI GAVTAITYST MVWVLSVSQP RPATISYEPL SMPSTSGSLF AVLNALGIIA
FAFRGHNLVL EIQSTMPSTF KHPAHVPMWR GAKISYFLIA LCIFPISIGG FWAYGNLMPS
GGMLAALYAF HIHDIPRGLL ATAFLLVVFS CLSSFQIYSM PAFDSFEAGY TSRTNKPCSI
WVRSGFRVFF GFVSFFIGVA LPFLSSLAGL LGGLTLPVTF AYPCFMWVLI KKPAKYSFNW
YFHWGLGWLG VAFSLAFSIG GIWSMVTNGL KLKFFKPPN
