LHWL1_ARATH
ID LHWL1_ARATH Reviewed; 734 AA.
AC P0C7P8; Q9LND3;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Transcription factor EMB1444 {ECO:0000303|PubMed:12586866};
DE AltName: Full=Basic helix-loop-helix protein 169 {ECO:0000305};
DE Short=AtbHLH169 {ECO:0000305};
DE Short=bHLH 169 {ECO:0000305};
DE AltName: Full=LONESOME HIGHWAY-like protein 1 {ECO:0000303|PubMed:17626058};
DE AltName: Full=Protein EMBRYO DEFECTIVE 1444 {ECO:0000303|PubMed:12586866};
DE AltName: Full=bHLH transcription factor bHLH169 {ECO:0000305};
GN Name=EMB1444 {ECO:0000303|PubMed:12586866}; Synonyms=BHLH169 {ECO:0000305};
GN OrderedLocusNames=At1g06150 {ECO:0000312|Araport:AT1G06150};
GN ORFNames=T21E18.20 {ECO:0000312|EMBL:AAF80138.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 571-734 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=12586866; DOI=10.1104/pp.014134;
RA Meinke D.W., Meinke L.K., Showalter T.C., Schissel A.M., Mueller L.A.,
RA Tzafrir I.;
RT "A sequence-based map of Arabidopsis genes with mutant phenotypes.";
RL Plant Physiol. 131:409-418(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=17626058; DOI=10.1242/dev.006296;
RA Ohashi-Ito K., Bergmann D.C.;
RT "Regulation of the Arabidopsis root vascular initial population by LONESOME
RT HIGHWAY.";
RL Development 134:2959-2968(2007).
CC -!- FUNCTION: Transcription factor that may regulate root development.
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P0C7P8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P0C7P8-2; Sequence=VSP_058848;
CC -!- DISRUPTION PHENOTYPE: Pale yellow-green embryos arrested at mature
CC cotyledon steps. {ECO:0000269|PubMed:12586866}.
CC -!- SIMILARITY: Belongs to the bHLH protein family. LHW subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF80138.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g06143 and At1g06150.; Evidence={ECO:0000305};
CC Sequence=BX816018; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AC024174; AAF80138.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27947.2; -; Genomic_DNA.
DR EMBL; CP002684; AEE27948.2; -; Genomic_DNA.
DR EMBL; BX816018; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; H86196; H86196.
DR RefSeq; NP_001184921.1; NM_001197992.2. [P0C7P8-1]
DR RefSeq; NP_001318931.1; NM_001331616.1. [P0C7P8-2]
DR AlphaFoldDB; P0C7P8; -.
DR SMR; P0C7P8; -.
DR STRING; 3702.AT1G06150.1; -.
DR PaxDb; P0C7P8; -.
DR PRIDE; P0C7P8; -.
DR ProteomicsDB; 238492; -. [P0C7P8-1]
DR EnsemblPlants; AT1G06150.1; AT1G06150.1; AT1G06150. [P0C7P8-1]
DR EnsemblPlants; AT1G06150.2; AT1G06150.2; AT1G06150. [P0C7P8-2]
DR GeneID; 6241227; -.
DR Gramene; AT1G06150.1; AT1G06150.1; AT1G06150. [P0C7P8-1]
DR Gramene; AT1G06150.2; AT1G06150.2; AT1G06150. [P0C7P8-2]
DR KEGG; ath:AT1G06150; -.
DR Araport; AT1G06150; -.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_013463_1_1_1; -.
DR InParanoid; P0C7P8; -.
DR OMA; KMQHKDT; -.
DR OrthoDB; 433891at2759; -.
DR PhylomeDB; P0C7P8; -.
DR PRO; PR:P0C7P8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P0C7P8; baseline and differential.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0048364; P:root development; ISS:UniProtKB.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR043561; LHW-like.
DR InterPro; IPR025610; MYC/MYB_N.
DR PANTHER; PTHR46196; PTHR46196; 1.
DR Pfam; PF14215; bHLH-MYC_N; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..734
FT /note="Transcription factor EMB1444"
FT /id="PRO_0000342603"
FT DOMAIN 552..601
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 537..566
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 548..555
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 543..566
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 618
FT /note="K -> KLQ (in isoform 2)"
FT /id="VSP_058848"
SQ SEQUENCE 734 AA; 81015 MW; 3FF766F598A6A81F CRC64;
MGYTLQQILR SICSNTDWNY AVFWKLNHHS PMVLTLEDVY CVNHERGLMP ESLHGGRHAH
DPLGLAVAKM SYHVHSLGEG IVGQVAISGQ HQWIFSEYLN DSHSTLQVHN GWESQISAGI
KTILIVAVGS CGVVQLGSLC KVEEDPALVT HIRHLFLALT DPLADHASNL MQCDINSPSD
RPKIPSKCLH EASPDFSGEF DKAMDMEGLN IVSQNTSNRS NDLPYNFTPT YFHMERTAQV
IGGLEAVQPS MFGSNDCVTS GFSVGVVDTK HKNQVDISDM SKVIYDEETG GYRYSRELDP
NFQHYSRNHV RNSGGTSALA MESDRLKAGS SYPQLDSTVL TALKTDKDYS RRNEVFQPSE
SQGSIFVKDT EHRQEEKSES SQLDALTASL CSFSGSELLE ALGPAFSKTS TDYGELAKFE
SAAAIRRTND MSHSHLTFES SSENLLDAVV ASMSNGDGNV RREISSSRST QSLLTTAEMA
QAEPFGHNKQ NIVSTVDSVI SQPPLADGLI QQNPSNICGA FSSIGFSSTC LSSSSDQFPT
SLEIPKKNKK RAKPGESSRP RPRDRQLIQD RIKELRELVP NGSKCSIDSL LECTIKHMLF
LQSVSQHADK LTKSASSKMQ HKDTGTLGIS STEQGSSWAV EIGGHLQVCS IMVENLDKEG
VMLIEMLCEE CSHFLEIANV IRSLELIILR GTTEKQGEKT WICFVVEGQN NKVMHRMDIL
WSLVQIFQPK ATNR
