LHWL3_ARATH
ID LHWL3_ARATH Reviewed; 720 AA.
AC Q58G01; F4IQS0; Q56WU6; Q84RJ4; Q8L7J0; Q9SJW8;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Transcription factor bHLH155;
DE AltName: Full=BHLH transcription factor gamma;
DE Short=bHLH gamma;
DE AltName: Full=Basic helix-loop-helix protein 155;
DE Short=AtbHLH155;
DE Short=bHLH 155;
DE AltName: Full=LONESOME HIGHWAY-like protein 3;
DE AltName: Full=bHLH transcription factor bHLH155;
GN Name=BHLH155; OrderedLocusNames=At2g31280; ORFNames=F16D14.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=12481096; DOI=10.1104/pp.010207;
RA Xiao Y.-L., Malik M., Whitelaw C.A., Town C.D.;
RT "Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2
RT of Arabidopsis.";
RL Plant Physiol. 130:2118-2128(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 452-720 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY.
RX PubMed=17626058; DOI=10.1242/dev.006296;
RA Ohashi-Ito K., Bergmann D.C.;
RT "Regulation of the Arabidopsis root vascular initial population by LONESOME
RT HIGHWAY.";
RL Development 134:2959-2968(2007).
CC -!- FUNCTION: Transcription factor that may regulate root development.
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- INTERACTION:
CC Q58G01; Q9FY69: BHLH143; NbExp=3; IntAct=EBI-15197899, EBI-15194247;
CC Q58G01; Q9FGB0: BHLH145; NbExp=3; IntAct=EBI-15197899, EBI-4477059;
CC Q58G01; Q9C7T4: BHLH96; NbExp=3; IntAct=EBI-15197899, EBI-15192637;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q58G01-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q58G01-2; Sequence=VSP_043890, VSP_043891, VSP_043892,
CC VSP_043893, VSP_043895;
CC Name=3;
CC IsoId=Q58G01-3; Sequence=VSP_043894;
CC -!- SIMILARITY: Belongs to the bHLH protein family. LHW subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD94375.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ576042; CAE09169.1; -; mRNA.
DR EMBL; AC006593; AAD20673.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08518.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08519.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08520.1; -; Genomic_DNA.
DR EMBL; AY129831; AAM97767.1; -; mRNA.
DR EMBL; AY231425; AAO86853.1; -; mRNA.
DR EMBL; AY954808; AAX55134.1; -; mRNA.
DR EMBL; AK221935; BAD94375.1; ALT_INIT; mRNA.
DR PIR; G84718; G84718.
DR RefSeq; NP_001031453.1; NM_001036376.1. [Q58G01-2]
DR RefSeq; NP_001031454.1; NM_001036377.1. [Q58G01-3]
DR RefSeq; NP_180686.2; NM_128684.5. [Q58G01-1]
DR AlphaFoldDB; Q58G01; -.
DR SMR; Q58G01; -.
DR BioGRID; 3033; 33.
DR IntAct; Q58G01; 33.
DR STRING; 3702.AT2G31280.3; -.
DR PaxDb; Q58G01; -.
DR PRIDE; Q58G01; -.
DR ProteomicsDB; 238493; -. [Q58G01-1]
DR EnsemblPlants; AT2G31280.1; AT2G31280.1; AT2G31280. [Q58G01-1]
DR EnsemblPlants; AT2G31280.2; AT2G31280.2; AT2G31280. [Q58G01-2]
DR EnsemblPlants; AT2G31280.3; AT2G31280.3; AT2G31280. [Q58G01-3]
DR GeneID; 817685; -.
DR Gramene; AT2G31280.1; AT2G31280.1; AT2G31280. [Q58G01-1]
DR Gramene; AT2G31280.2; AT2G31280.2; AT2G31280. [Q58G01-2]
DR Gramene; AT2G31280.3; AT2G31280.3; AT2G31280. [Q58G01-3]
DR KEGG; ath:AT2G31280; -.
DR Araport; AT2G31280; -.
DR TAIR; locus:2042551; AT2G31280.
DR eggNOG; ENOG502QSH7; Eukaryota.
DR InParanoid; Q58G01; -.
DR OMA; LCFNTEW; -.
DR OrthoDB; 433891at2759; -.
DR PhylomeDB; Q58G01; -.
DR PRO; PR:Q58G01; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q58G01; baseline and differential.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0048364; P:root development; ISS:UniProtKB.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR043561; LHW-like.
DR InterPro; IPR025610; MYC/MYB_N.
DR PANTHER; PTHR46196; PTHR46196; 2.
DR Pfam; PF14215; bHLH-MYC_N; 2.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..720
FT /note="Transcription factor bHLH155"
FT /id="PRO_0000417699"
FT DOMAIN 544..593
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 522..558
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 540..547
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 535..558
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 3..6
FT /note="STSQ -> KRQISQDEVGPPIKPRAGLRREQAGRGSYR (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:14600211"
FT /id="VSP_043890"
FT VAR_SEQ 101..114
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14600211"
FT /id="VSP_043891"
FT VAR_SEQ 133..174
FT /note="CKVNEDVNFVNHIRHLFLALRDPLADHAANLRQCNMNNSLCL -> CK (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14600211"
FT /id="VSP_043892"
FT VAR_SEQ 650
FT /note="E -> EFNLCLNSSPKFCECVLKVFLGIGQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14600211"
FT /id="VSP_043893"
FT VAR_SEQ 692
FT /note="E -> EVGSRITQFMKEIPKQIK (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_043894"
FT VAR_SEQ 693..720
FT /note="SQNSKVMQRMDILWSLVQIFQPKANEKG -> VGSRITQFMKEIPKQIKVFS
FT LVLIELWISMIYMTD (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14600211"
FT /id="VSP_043895"
FT CONFLICT 612
FT /note="Q -> L (in Ref. 4; AAM97767)"
FT /evidence="ECO:0000305"
FT CONFLICT 644
FT /note="Q -> H (in Ref. 4; AAM97767)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 720 AA; 79809 MW; FA885575F8EB9173 CRC64;
MGSTSQEILK SFCFNTDWDY AVFWQLNHRG SRMVLTLEDA YYDHHGTNMH GAHDPLGLAV
AKMSYHVYSL GEGIVGQVAV SGEHQWVFPE NYNNCNSAFE FHNVWESQIS AGIKTILVVA
VGPCGVVQLG SLCKVNEDVN FVNHIRHLFL ALRDPLADHA ANLRQCNMNN SLCLPKMPSE
GLHAEAFPDC SGEVDKAMDV EESNILTQYK TRRSDSMPYN TPSSCLVMEK AAQVVGGREV
VQGSTCGSYS GVTFGFPVDL VGAKHENQVG TNIIRDAPHV GMTSGCKDSR DLDPNLHLYM
KNHVLNDTST SALAIEAERL ITSQSYPRLD STFQATSRTD KESSYHNEVF QLSENQGNKY
IKETERMLGR NCESSQFDAL ISSGYTFAGS ELLEALGSAF KQTNTGQEEL LKSEHGSTMR
PTDDMSHSQL TFDPGPENLL DAVVANVCQR DGNARDDMMS SRSVQSLLTN MELAEPSGQK
KHNIVNPINS AMNQPPMAEV DTQQNSSDIC GAFSSIGFSS TYPSSSSDQF QTSLDIPKKN
KKRAKPGESS RPRPRDRQLI QDRIKELREL VPNGSKCSID SLLERTIKHM LFLQNVTKHA
EKLSKSANEK MQQKETGMQG SSCAVEVGGH LQVSSIIVEN LNKQGMVLIE MLCEECGHFL
EIANVIRSLD LVILRGFTET QGEKTWICFV TESQNSKVMQ RMDILWSLVQ IFQPKANEKG
