LHW_ARATH
ID LHW_ARATH Reviewed; 650 AA.
AC Q9XIN0; B9DGI1; Q0WMN8;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Transcription factor LHW;
DE AltName: Full=BHLH transcription factor delta;
DE Short=bHLH delta;
DE AltName: Full=Basic helix-loop-helix protein 156;
DE Short=AtbHLH156;
DE Short=bHLH 156;
DE AltName: Full=Protein LONESOME HIGHWAY;
DE AltName: Full=bHLH transcription factor bHLH156;
GN Name=LHW; Synonyms=BHLH156; OrderedLocusNames=At2g27230; ORFNames=F12K2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 332-650.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP HOMODIMER, AND SUBUNIT.
RX PubMed=17626058; DOI=10.1242/dev.006296;
RA Ohashi-Ito K., Bergmann D.C.;
RT "Regulation of the Arabidopsis root vascular initial population by LONESOME
RT HIGHWAY.";
RL Development 134:2959-2968(2007).
CC -!- FUNCTION: Transcription activator that regulates root development;
CC promotes the production of stele cells in roots. Coordinately controls
CC the number of all vascular cell types by regulating the size of the
CC pool of cells from which they arise. {ECO:0000269|PubMed:17626058}.
CC -!- SUBUNIT: Homodimer. Can also interact with bHLH proteins.
CC {ECO:0000269|PubMed:17626058}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:17626058}.
CC -!- TISSUE SPECIFICITY: Expressed in both root and shoot meristems. Present
CC in root tips. {ECO:0000269|PubMed:17626058}.
CC -!- DISRUPTION PHENOTYPE: Loss of bilateral symmetry and reduced number of
CC cells in the center of the root, resulting in roots with only single
CC xylem and phloem poles and defect in lateral root formation.
CC {ECO:0000269|PubMed:17626058}.
CC -!- SIMILARITY: Belongs to the bHLH protein family. LHW subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ576043; CAE09170.1; -; mRNA.
DR EMBL; AC006233; AAD42006.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07959.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07960.1; -; Genomic_DNA.
DR EMBL; AB493566; BAH30404.1; -; mRNA.
DR EMBL; AK317162; BAH19848.1; -; mRNA.
DR EMBL; AK229779; BAF01612.1; -; mRNA.
DR PIR; D84670; D84670.
DR RefSeq; NP_001031430.1; NM_001036353.1.
DR RefSeq; NP_565640.1; NM_128281.2.
DR AlphaFoldDB; Q9XIN0; -.
DR SMR; Q9XIN0; -.
DR BioGRID; 2617; 21.
DR IntAct; Q9XIN0; 13.
DR STRING; 3702.AT2G27230.2; -.
DR PaxDb; Q9XIN0; -.
DR PRIDE; Q9XIN0; -.
DR ProteomicsDB; 238437; -.
DR EnsemblPlants; AT2G27230.1; AT2G27230.1; AT2G27230.
DR EnsemblPlants; AT2G27230.2; AT2G27230.2; AT2G27230.
DR GeneID; 817265; -.
DR Gramene; AT2G27230.1; AT2G27230.1; AT2G27230.
DR Gramene; AT2G27230.2; AT2G27230.2; AT2G27230.
DR KEGG; ath:AT2G27230; -.
DR Araport; AT2G27230; -.
DR TAIR; locus:2039580; AT2G27230.
DR eggNOG; ENOG502QSGF; Eukaryota.
DR HOGENOM; CLU_013463_1_1_1; -.
DR InParanoid; Q9XIN0; -.
DR OMA; MMESQIN; -.
DR OrthoDB; 815694at2759; -.
DR PhylomeDB; Q9XIN0; -.
DR PRO; PR:Q9XIN0; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9XIN0; baseline and differential.
DR Genevisible; Q9XIN0; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0042803; F:protein homodimerization activity; IPI:TAIR.
DR GO; GO:0010078; P:maintenance of root meristem identity; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0010479; P:stele development; IMP:TAIR.
DR GO; GO:0010089; P:xylem development; IGI:TAIR.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR033260; LHW.
DR InterPro; IPR043561; LHW-like.
DR InterPro; IPR025610; MYC/MYB_N.
DR PANTHER; PTHR46196; PTHR46196; 2.
DR PANTHER; PTHR46196:SF4; PTHR46196:SF4; 2.
DR Pfam; PF14215; bHLH-MYC_N; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..650
FT /note="Transcription factor LHW"
FT /id="PRO_0000417698"
FT DOMAIN 455..504
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 381..417
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 431..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 451..458
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 381..403
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 447..470
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 168
FT /note="G -> R (in Ref. 5; BAH19848)"
FT /evidence="ECO:0000305"
FT CONFLICT 513
FT /note="K -> E (in Ref. 5; BAH19848)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 650 AA; 71771 MW; 10087EA7B2E80B06 CRC64;
MGVLLREALR SMCVNNQWSY AVFWKIGCQN SSLLIWEECY NETESSSNPR RLCGLGVDTQ
GNEKVQLLTN RMMLNNRIIL VGEGLVGRAA FTGHHQWILA NSFNRDVHPP EVINEMLLQF
SAGIQTVAVF PVVPHGVVQL GSSLPIMENL GFVNDVKGLI LQLGCVPGAL LSENYRTYEP
AADFIGVPVS RIIPSQGHKI LQSSAFVAET SKQHFNSTGS SDHQMVEESP CNLVDEHEGG
WQSTTGFLTA GEVAVPSNPD AWLNQNFSCM SNVDAAEQQQ IPCEDISSKR SLGSDDLFDM
LGLDDKNKGC DNSWGVSQMR TEVLTRELSD FRIIQEMDPE FGSSGYELSG TDHLLDAVVS
GACSSTKQIS DETSESCKTT LTKVSNSSVT TPSHSSPQGS QLFEKKHGQP LGPSSVYGSQ
ISSWVEQAHS LKREGSPRMV NKNETAKPAN NRKRLKPGEN PRPRPKDRQM IQDRVKELRE
IIPNGAKCSI DALLERTIKH MLFLQNVSKH SDKLKQTGES KIMKEDGGGA TWAFEVGSKS
MVCPIVVEDI NPPRIFQVEM LCEQRGFFLE IADWIRSLGL TILKGVIETR VDKIWARFTV
EASRDVTRME IFMQLVNILE QTMKCGGNSK TILDGIKATM PLPVTGGCSM
