LHX2_HUMAN
ID LHX2_HUMAN Reviewed; 406 AA.
AC P50458; O95860; Q52M57; Q8N1Z3;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 187.
DE RecName: Full=LIM/homeobox protein Lhx2;
DE Short=Homeobox protein LH-2;
DE Short=LIM homeobox protein 2;
GN Name=LHX2; Synonyms=LH2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Placenta;
RX PubMed=8649822;
RA Wu H.-K., Heng H.H.Q., Siderovski D.P., Dong W.-F., Okuno Y., Shi X.-M.,
RA Tsui L.-C., Minden M.D.;
RT "Identification of a human LIM-Hox gene, hLH-2, aberrantly expressed in
RT chronic myelogenous leukaemia and located on 9q33-34.1.";
RL Oncogene 12:1205-1212(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-406.
RC TISSUE=Brain cortex;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 18-406.
RX PubMed=10051612; DOI=10.1073/pnas.96.5.2165;
RA Rincon-Limas D.E., Lu C.-H., Canal I., Calleja M., Rodriguez-Esteban C.,
RA Izpisua-Belmonte J.-C., Botas J.;
RT "Conservation of the expression and function of apterous orthologs in
RT Drosophila and mammals.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:2165-2170(1999).
CC -!- FUNCTION: Acts as a transcriptional activator. Stimulates the promoter
CC of the alpha-glycoprotein gene. Transcriptional regulatory protein
CC involved in the control of cell differentiation in developing lymphoid
CC and neural cell types (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts (via LIM domains) with CITED2. Interacts with
CC POU4F2. {ECO:0000250|UniProtKB:Q9Z0S2}.
CC -!- INTERACTION:
CC P50458; Q49AR9: ANKS1A; NbExp=3; IntAct=EBI-12179869, EBI-11954519;
CC P50458; A7KAX9: ARHGAP32; NbExp=3; IntAct=EBI-12179869, EBI-308663;
CC P50458; Q8NDZ0: BEND2; NbExp=3; IntAct=EBI-12179869, EBI-954079;
CC P50458; Q4VC44: FLYWCH1; NbExp=3; IntAct=EBI-12179869, EBI-719415;
CC P50458; Q9NSC5: HOMER3; NbExp=3; IntAct=EBI-12179869, EBI-748420;
CC P50458; P31273: HOXC8; NbExp=3; IntAct=EBI-12179869, EBI-1752118;
CC P50458; P50053-2: KHK; NbExp=3; IntAct=EBI-12179869, EBI-12204387;
CC P50458; Q86U70: LDB1; NbExp=3; IntAct=EBI-12179869, EBI-677177;
CC P50458; Q86U70-2: LDB1; NbExp=3; IntAct=EBI-12179869, EBI-11979761;
CC P50458; Q3ZCW2: LGALSL; NbExp=3; IntAct=EBI-12179869, EBI-10241423;
CC P50458; Q8TBB1: LNX1; NbExp=3; IntAct=EBI-12179869, EBI-739832;
CC P50458; Q17RB8: LONRF1; NbExp=3; IntAct=EBI-12179869, EBI-2341787;
CC P50458; Q9UBU8-2: MORF4L1; NbExp=3; IntAct=EBI-12179869, EBI-10288852;
CC P50458; Q6PF18: MORN3; NbExp=3; IntAct=EBI-12179869, EBI-9675802;
CC P50458; Q99650: OSMR; NbExp=3; IntAct=EBI-12179869, EBI-2804080;
CC P50458; Q12837: POU4F2; NbExp=3; IntAct=EBI-12179869, EBI-17236143;
CC P50458; Q9Y3C6: PPIL1; NbExp=3; IntAct=EBI-12179869, EBI-2557649;
CC P50458; Q8N8B7-2: TCEANC; NbExp=3; IntAct=EBI-12179869, EBI-11955057;
CC P50458; Q9NUJ3: TCP11L1; NbExp=3; IntAct=EBI-12179869, EBI-2555179;
CC P50458; Q9UKI8: TLK1; NbExp=3; IntAct=EBI-12179869, EBI-740492;
CC P50458; Q9UL33-2: TRAPPC2L; NbExp=3; IntAct=EBI-12179869, EBI-11119202;
CC P50458; P0CB47: UBTFL1; NbExp=3; IntAct=EBI-12179869, EBI-17208936;
CC P50458; Q96MM3: ZFP42; NbExp=3; IntAct=EBI-12179869, EBI-12151755;
CC P50458; Q8NB15: ZNF511; NbExp=3; IntAct=EBI-12179869, EBI-10269136;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DOMAIN: LIM domains are necessary for transcription activation.
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC04371.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U11701; AAB08752.1; -; mRNA.
DR EMBL; BC093662; AAH93662.1; -; mRNA.
DR EMBL; BC112185; AAI12186.1; -; mRNA.
DR EMBL; AK094511; BAC04371.1; ALT_INIT; mRNA.
DR EMBL; AF124735; AAD20013.1; -; mRNA.
DR CCDS; CCDS6853.1; -.
DR RefSeq; NP_004780.3; NM_004789.3.
DR AlphaFoldDB; P50458; -.
DR BMRB; P50458; -.
DR SMR; P50458; -.
DR BioGRID; 114758; 177.
DR IntAct; P50458; 171.
DR MINT; P50458; -.
DR STRING; 9606.ENSP00000362717; -.
DR iPTMnet; P50458; -.
DR PhosphoSitePlus; P50458; -.
DR BioMuta; LHX2; -.
DR DMDM; 8247936; -.
DR jPOST; P50458; -.
DR MassIVE; P50458; -.
DR PaxDb; P50458; -.
DR PeptideAtlas; P50458; -.
DR PRIDE; P50458; -.
DR ProteomicsDB; 56230; -.
DR Antibodypedia; 16286; 384 antibodies from 35 providers.
DR DNASU; 9355; -.
DR Ensembl; ENST00000373615.9; ENSP00000362717.4; ENSG00000106689.11.
DR GeneID; 9355; -.
DR KEGG; hsa:9355; -.
DR MANE-Select; ENST00000373615.9; ENSP00000362717.4; NM_004789.4; NP_004780.3.
DR UCSC; uc004boe.2; human.
DR CTD; 9355; -.
DR DisGeNET; 9355; -.
DR GeneCards; LHX2; -.
DR HGNC; HGNC:6594; LHX2.
DR HPA; ENSG00000106689; Group enriched (brain, choroid plexus, retina).
DR MIM; 603759; gene.
DR neXtProt; NX_P50458; -.
DR OpenTargets; ENSG00000106689; -.
DR PharmGKB; PA30365; -.
DR VEuPathDB; HostDB:ENSG00000106689; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000158540; -.
DR HOGENOM; CLU_027802_4_1_1; -.
DR InParanoid; P50458; -.
DR OMA; AIFHLEC; -.
DR OrthoDB; 1174930at2759; -.
DR PhylomeDB; P50458; -.
DR TreeFam; TF315442; -.
DR PathwayCommons; P50458; -.
DR Reactome; R-HSA-9010553; Regulation of expression of SLITs and ROBOs.
DR Reactome; R-HSA-9752946; Expression and translocation of olfactory receptors.
DR SignaLink; P50458; -.
DR SIGNOR; P50458; -.
DR BioGRID-ORCS; 9355; 7 hits in 1092 CRISPR screens.
DR ChiTaRS; LHX2; human.
DR GeneWiki; LHX2; -.
DR GenomeRNAi; 9355; -.
DR Pharos; P50458; Tbio.
DR PRO; PR:P50458; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; P50458; protein.
DR Bgee; ENSG00000106689; Expressed in ventricular zone and 117 other tissues.
DR ExpressionAtlas; P50458; baseline and differential.
DR Genevisible; P50458; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IEA:Ensembl.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IEA:Ensembl.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048675; P:axon extension; IEA:Ensembl.
DR GO; GO:0007411; P:axon guidance; IEA:Ensembl.
DR GO; GO:0021987; P:cerebral cortex development; IEA:Ensembl.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IEA:Ensembl.
DR GO; GO:0001942; P:hair follicle development; IEA:Ensembl.
DR GO; GO:0045199; P:maintenance of epithelial cell apical/basal polarity; IEA:Ensembl.
DR GO; GO:0007498; P:mesoderm development; IEA:Ensembl.
DR GO; GO:0045814; P:negative regulation of gene expression, epigenetic; IEA:Ensembl.
DR GO; GO:0050768; P:negative regulation of neurogenesis; IEA:Ensembl.
DR GO; GO:2000678; P:negative regulation of transcription regulatory region DNA binding; IEA:Ensembl.
DR GO; GO:0001843; P:neural tube closure; IEA:Ensembl.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0021772; P:olfactory bulb development; IEA:Ensembl.
DR GO; GO:2000179; P:positive regulation of neural precursor cell proliferation; IEA:Ensembl.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060041; P:retina development in camera-type eye; IEA:Ensembl.
DR GO; GO:0021978; P:telencephalon regionalization; IEA:Ensembl.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Homeobox; LIM domain; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..406
FT /note="LIM/homeobox protein Lhx2"
FT /id="PRO_0000075778"
FT DOMAIN 53..105
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 115..168
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 266..325
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 250..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 328..374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 387..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 307..323
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 330..374
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 22..28
FT /note="AKSEAPA -> QERGSR (in Ref. 1; AAB08752)"
FT /evidence="ECO:0000305"
FT CONFLICT 54
FT /note="A -> G (in Ref. 1; AAB08752)"
FT /evidence="ECO:0000305"
FT CONFLICT 107
FT /note="Y -> YSPSLHGPY (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 187
FT /note="A -> Q (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 190..192
FT /note="AAA -> RAR (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="Missing (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 241..242
FT /note="NA -> TR (in Ref. 1; AAB08752)"
FT /evidence="ECO:0000305"
FT CONFLICT 391..406
FT /note="GHEPHSPSQTTLTNLF -> AMSLTAPHKRLLPTFSNDSQPPHPTISLKKKL
FT SLV (in Ref. 1; AAB08752)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 406 AA; 44373 MW; DF73AAC81867D30D CRC64;
MLFHSLSGPE VHGVIDEMDR RAKSEAPAIS SAIDRGDTET TMPSISSDRA ALCAGCGGKI
SDRYYLLAVD KQWHMRCLKC CECKLNLESE LTCFSKDGSI YCKEDYYRRF SVQRCARCHL
GISASEMVMR ARDLVYHLNC FTCTTCNKML TTGDHFGMKD SLVYCRLHFE ALLQGEYPAH
FNHADVAAAA AAAAAAKSAG LGAAGANPLG LPYYNGVGTV QKGRPRKRKS PGPGADLAAY
NAALSCNEND AEHLDRDQPY PSSQKTKRMR TSFKHHQLRT MKSYFAINHN PDAKDLKQLA
QKTGLTKRVL QVWFQNARAK FRRNLLRQEN TGVDKSTDAA LQTGTPSGPA SELSNASLSP
SSTPTTLTDL TSPTLPTVTS VLTSVPGNLE GHEPHSPSQT TLTNLF
