ID   LHX3_XENLA              Reviewed;         395 AA.
AC   P36200;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=LIM/homeobox protein Lhx3;
DE            Short=LIM homeobox protein 3;
DE   AltName: Full=Homeobox protein LIM-3;
DE            Short=xLIM-3;
GN   Name=lhx3; Synonyms=lim3;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=8103491; DOI=10.1006/dbio.1993.1237;
RA   Taira M., Hayes W.P., Otani H., Dawid I.B.;
RT   "Expression of LIM class homeobox gene Xlim-3 in Xenopus development is
RT   limited to neural and neuroendocrine tissues.";
RL   Dev. Biol. 159:245-256(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 162-200.
RX   PubMed=1347750; DOI=10.1101/gad.6.3.356;
RA   Taira M., Jamrich M., Good P.J., Dawid I.B.;
RT   "The LIM domain-containing homeo box gene Xlim-1 is expressed specifically
RT   in the organizer region of Xenopus gastrula embryos.";
RL   Genes Dev. 6:356-366(1992).
RN   [3]
RP   INTERACTION WITH LDB1 AND RNF12.
RX   PubMed=12874135; DOI=10.1242/dev.00621;
RA   Hiratani I., Yamamoto N., Mochizuki T., Ohmori S.-Y., Taira M.;
RT   "Selective degradation of excess Ldb1 by Rnf12/RLIM confers proper Ldb1
RT   expression levels and Xlim-1/Ldb1 stoichiometry in Xenopus organizer
RT   functions.";
RL   Development 130:4161-4175(2003).
CC   -!- FUNCTION: May be involved in the specification and maintenance of
CC       differentiation of distinct neuronal and neuroendocrine tissues. Early
CC       marker for the pituitary and pineal lineages, it may be involved in
CC       specifying these lineages.
CC   -!- SUBUNIT: Interacts with ldb1 and with the N-terminus of rnf12.
CC       {ECO:0000269|PubMed:12874135}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: In dorsal regions at neural tube and tailbud stages
CC       and in adults predominantly in the pituitary gland and weakly in the
CC       eye and brain.
CC   -!- DEVELOPMENTAL STAGE: First detectable at the neural plate stage in the
CC       stomodeal-hypophyseal (pituitary) anlage and in the neural plate. At
CC       later stages it persists in the pituitary and pineal, retina, hindbrain
CC       and spinal cord.
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DR   EMBL; Z22702; CAA80402.1; -; mRNA.
DR   EMBL; Z11589; CAA77674.1; -; mRNA.
DR   PIR; S38821; S38821.
DR   RefSeq; NP_001081623.1; NM_001088154.1.
DR   AlphaFoldDB; P36200; -.
DR   SMR; P36200; -.
DR   GeneID; 397959; -.
DR   KEGG; xla:397959; -.
DR   CTD; 397959; -.
DR   Xenbase; XB-GENE-865930; lhx3.L.
DR   OrthoDB; 1174754at2759; -.
DR   Proteomes; UP000186698; Chromosome 8L.
DR   Bgee; 397959; Expressed in camera-type eye and 4 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0001221; F:transcription coregulator binding; IPI:UniProtKB.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR001781; Znf_LIM.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00412; LIM; 2.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00132; LIM; 2.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR   PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Homeobox; LIM domain; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..395
FT                   /note="LIM/homeobox protein Lhx3"
FT                   /id="PRO_0000075786"
FT   DOMAIN          28..78
FT                   /note="LIM zinc-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          87..141
FT                   /note="LIM zinc-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DNA_BIND        154..213
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          208..304
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          363..383
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        231..246
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        255..285
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        174
FT                   /note="N -> D (in Ref. 2; CAA77674)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   395 AA;  44031 MW;  31A97C70A683C539 CRC64;
     MLLERVRTGT QKSSDMCGYT GSPEIPQCAG CNQHIVDRFI LKVLDRHWHS KCLKCNDCQI
     QLAEKCFSRG DSVYCKDDFF KRFGTKCAAC QQGIPPTQVV RRAQEFVYHL HCFACIVCKR
     QLATGDEFYL MEDSRLVCKA DYETAKQREA ESTAKRPRTT ITAKQLETLK NAYNNSPKPA
     RHVREQLSSE TGLDMRVVQV WFQNRRAKEK RLKKDAGRQR WGQYFRNMKR SRGNSKSDKD
     SIQEEGPDSD AEVSFTDEPS MSEMNHSNGI YNSLNDSSPV LGRQAGSNGP FSLEHGGIPT
     QDQYHNLRSN SPYGIPQSPA SLQSMPGHQS LLSNLAFPDT GLGIIGQGGQ GVAPTMRVIG
     VNGPSSDLST GSSGGYPDFP VSPASWLDEV DHTQF