LHX8_HUMAN
ID LHX8_HUMAN Reviewed; 356 AA.
AC Q68G74; E9PGE3;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=LIM/homeobox protein Lhx8;
DE Short=LIM homeobox protein 8;
GN Name=LHX8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcription factor involved in differentiation of certain
CC neurons and mesenchymal cells. {ECO:0000250}.
CC -!- INTERACTION:
CC Q68G74; Q9NYB9-2: ABI2; NbExp=3; IntAct=EBI-8474075, EBI-11096309;
CC Q68G74; P15336: ATF2; NbExp=3; IntAct=EBI-8474075, EBI-1170906;
CC Q68G74; Q96QL7: ATF6B; NbExp=3; IntAct=EBI-8474075, EBI-12015400;
CC Q68G74; A0A0S2Z4M1: AXIN1; NbExp=3; IntAct=EBI-8474075, EBI-16429430;
CC Q68G74; Q8N9N5-2: BANP; NbExp=3; IntAct=EBI-8474075, EBI-11524452;
CC Q68G74; Q8TD31-3: CCHCR1; NbExp=3; IntAct=EBI-8474075, EBI-10175300;
CC Q68G74; P48643: CCT5; NbExp=3; IntAct=EBI-8474075, EBI-355710;
CC Q68G74; P68400: CSNK2A1; NbExp=3; IntAct=EBI-8474075, EBI-347804;
CC Q68G74; G5E9A7: DMWD; NbExp=3; IntAct=EBI-8474075, EBI-10976677;
CC Q68G74; O43281-2: EFS; NbExp=3; IntAct=EBI-8474075, EBI-11525448;
CC Q68G74; P22607: FGFR3; NbExp=3; IntAct=EBI-8474075, EBI-348399;
CC Q68G74; Q6QHK4: FIGLA; NbExp=8; IntAct=EBI-8474075, EBI-11976617;
CC Q68G74; P15408: FOSL2; NbExp=3; IntAct=EBI-8474075, EBI-3893419;
CC Q68G74; O15353: FOXN1; NbExp=3; IntAct=EBI-8474075, EBI-11319000;
CC Q68G74; Q9BRT9: GINS4; NbExp=3; IntAct=EBI-8474075, EBI-747500;
CC Q68G74; Q53GS7: GLE1; NbExp=3; IntAct=EBI-8474075, EBI-1955541;
CC Q68G74; Q14957: GRIN2C; NbExp=3; IntAct=EBI-8474075, EBI-8285963;
CC Q68G74; P28799: GRN; NbExp=3; IntAct=EBI-8474075, EBI-747754;
CC Q68G74; P42858: HTT; NbExp=12; IntAct=EBI-8474075, EBI-466029;
CC Q68G74; O60333-2: KIF1B; NbExp=3; IntAct=EBI-8474075, EBI-10975473;
CC Q68G74; P57682: KLF3; NbExp=3; IntAct=EBI-8474075, EBI-8472267;
CC Q68G74; P78386: KRT85; NbExp=3; IntAct=EBI-8474075, EBI-1049371;
CC Q68G74; Q86U70: LDB1; NbExp=5; IntAct=EBI-8474075, EBI-677177;
CC Q68G74; Q86U70-2: LDB1; NbExp=6; IntAct=EBI-8474075, EBI-11979761;
CC Q68G74; O43679: LDB2; NbExp=5; IntAct=EBI-8474075, EBI-2865580;
CC Q68G74; Q14511-2: NEDD9; NbExp=3; IntAct=EBI-8474075, EBI-11746523;
CC Q68G74; Q86Y26: NUTM1; NbExp=3; IntAct=EBI-8474075, EBI-10178410;
CC Q68G74; O43933: PEX1; NbExp=3; IntAct=EBI-8474075, EBI-988601;
CC Q68G74; Q8WWY3: PRPF31; NbExp=3; IntAct=EBI-8474075, EBI-1567797;
CC Q68G74; Q9Y3C5: RNF11; NbExp=3; IntAct=EBI-8474075, EBI-396669;
CC Q68G74; Q8IZE3: SCYL3; NbExp=3; IntAct=EBI-8474075, EBI-1380680;
CC Q68G74; A0A0B4J2F2: SIK1B; NbExp=3; IntAct=EBI-8474075, EBI-22345187;
CC Q68G74; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-8474075, EBI-5235340;
CC Q68G74; Q86TI0: TBC1D1; NbExp=3; IntAct=EBI-8474075, EBI-1644036;
CC Q68G74; Q9NU19: TBC1D22B; NbExp=3; IntAct=EBI-8474075, EBI-8787464;
CC Q68G74; Q86WV8: TSC1; NbExp=3; IntAct=EBI-8474075, EBI-12806590;
CC Q68G74; Q9UMX0: UBQLN1; NbExp=3; IntAct=EBI-8474075, EBI-741480;
CC Q68G74; O76024: WFS1; NbExp=3; IntAct=EBI-8474075, EBI-720609;
CC Q68G74; O43167: ZBTB24; NbExp=3; IntAct=EBI-8474075, EBI-744471;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q68G74-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q68G74-2; Sequence=VSP_047378;
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DR EMBL; AC099786; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC040321; AAH40321.1; -; mRNA.
DR CCDS; CCDS30756.1; -. [Q68G74-1]
DR CCDS; CCDS58008.1; -. [Q68G74-2]
DR RefSeq; NP_001001933.1; NM_001001933.1. [Q68G74-1]
DR RefSeq; NP_001243043.1; NM_001256114.1. [Q68G74-2]
DR AlphaFoldDB; Q68G74; -.
DR SMR; Q68G74; -.
DR BioGRID; 136112; 51.
DR IntAct; Q68G74; 65.
DR MINT; Q68G74; -.
DR STRING; 9606.ENSP00000294638; -.
DR iPTMnet; Q68G74; -.
DR PhosphoSitePlus; Q68G74; -.
DR BioMuta; LHX8; -.
DR DMDM; 296434566; -.
DR MassIVE; Q68G74; -.
DR PaxDb; Q68G74; -.
DR PeptideAtlas; Q68G74; -.
DR PRIDE; Q68G74; -.
DR Antibodypedia; 33466; 211 antibodies from 27 providers.
DR DNASU; 431707; -.
DR Ensembl; ENST00000294638.9; ENSP00000294638.5; ENSG00000162624.15. [Q68G74-1]
DR Ensembl; ENST00000356261.4; ENSP00000348597.3; ENSG00000162624.15. [Q68G74-2]
DR GeneID; 431707; -.
DR KEGG; hsa:431707; -.
DR MANE-Select; ENST00000356261.4; ENSP00000348597.3; NM_001256114.2; NP_001243043.1. [Q68G74-2]
DR UCSC; uc001dgo.3; human. [Q68G74-1]
DR CTD; 431707; -.
DR DisGeNET; 431707; -.
DR GeneCards; LHX8; -.
DR HGNC; HGNC:28838; LHX8.
DR HPA; ENSG00000162624; Tissue enhanced (brain, lymphoid tissue, salivary gland).
DR MIM; 604425; gene.
DR neXtProt; NX_Q68G74; -.
DR OpenTargets; ENSG00000162624; -.
DR PharmGKB; PA142671553; -.
DR VEuPathDB; HostDB:ENSG00000162624; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000156200; -.
DR HOGENOM; CLU_027802_1_0_1; -.
DR InParanoid; Q68G74; -.
DR OMA; ELLVMCE; -.
DR OrthoDB; 1161127at2759; -.
DR PhylomeDB; Q68G74; -.
DR TreeFam; TF315442; -.
DR PathwayCommons; Q68G74; -.
DR SignaLink; Q68G74; -.
DR SIGNOR; Q68G74; -.
DR BioGRID-ORCS; 431707; 8 hits in 1090 CRISPR screens.
DR ChiTaRS; LHX8; human.
DR GenomeRNAi; 431707; -.
DR Pharos; Q68G74; Tbio.
DR PRO; PR:Q68G74; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q68G74; protein.
DR Bgee; ENSG00000162624; Expressed in secondary oocyte and 49 other tissues.
DR Genevisible; Q68G74; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0001674; C:female germ cell nucleus; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0008585; P:female gonad development; IEA:Ensembl.
DR GO; GO:0021884; P:forebrain neuron development; IBA:GO_Central.
DR GO; GO:0007611; P:learning or memory; IEA:Ensembl.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:Ensembl.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Homeobox; LIM domain; Metal-binding;
KW Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..356
FT /note="LIM/homeobox protein Lhx8"
FT /id="PRO_0000295810"
FT DOMAIN 73..133
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 134..196
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 225..284
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 23..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..10
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_047378"
FT CONFLICT 297
FT /note="V -> A (in Ref. 2; AAH40321)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 356 AA; 39301 MW; 9BA025D3ECADCD56 CRC64;
MQILSRCQGL MSEECGRTTA LAAGRTRKGA GEEGLVSPEG AGDEDSCSSS APLSPSSSPR
SMASGSGCPP GKCVCNSCGL EIVDKYLLKV NDLCWHVRCL SCSVCRTSLG RHTSCYIKDK
DIFCKLDYFR RYGTRCSRCG RHIHSTDWVR RAKGNVYHLA CFACFSCKRQ LSTGEEFALV
EEKVLCRVHY DCMLDNLKRE VENGNGISVE GALLTEQDVN HPKPAKRART SFTADQLQVM
QAQFAQDNNP DAQTLQKLAE RTGLSRRVIQ VWFQNCRARH KKHVSPNHSS STPVTAVPPS
RLSPPMLEEM AYSAYVPQDG TMLTALHSYM DAHSPTTLGL QPLLPHSMTQ LPISHT
