LHX8_MOUSE
ID LHX8_MOUSE Reviewed; 367 AA.
AC O35652; O70163; O88707; Q3TMT2;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 4.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=LIM/homeobox protein Lhx8;
DE Short=LIM homeobox protein 8;
DE AltName: Full=L3;
DE AltName: Full=LIM/homeobox protein Lhx7;
DE Short=LIM homeobox protein 7;
GN Name=Lhx8; Synonyms=Lhx7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=ICR; TISSUE=Brain;
RX PubMed=8929991; DOI=10.1016/0304-3940(96)12341-7;
RA Matsumoto K., Tanaka T., Furuyama T., Kashihara Y., Mori T., Ishii N.,
RA Kitanaka J., Takemura M., Tohyama M., Wanaka A.;
RT "L3, a novel murine LIM-homeodomain transcription factor expressed in the
RT ventral telencephalon and the mesenchyme surrounding the oral cavity.";
RL Neurosci. Lett. 204:113-116(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ; TISSUE=Liver;
RX PubMed=9598319; DOI=10.1006/geno.1998.5203;
RA Kitanaka J., Takemura M., Matsumoto K., Mori T., Wanaka A.;
RT "Structure and chromosomal localization of a murine LIM/homeobox gene,
RT Lhx8.";
RL Genomics 49:307-309(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9570771; DOI=10.1242/dev.125.11.2063;
RA Grigoriou M., Tucker A.S., Sharpe P.T., Pachnis V.;
RT "Expression and regulation of Lhx6 and Lhx7, a novel subfamily of LIM
RT homeodomain encoding genes, suggests a role in mammalian head
RT development.";
RL Development 125:2063-2074(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Lymph node;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcription factor involved in differentiation of certain
CC neurons and mesenchymal cells.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Limited spatially to the medical ganglionic
CC eminence and the mesenchyme surrounding the oral cavity and temporally
CC from middle embryonic to early postnatal development.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA21649.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; D49658; BAA21649.1; ALT_INIT; mRNA.
DR EMBL; AB007596; BAA28628.1; -; Genomic_DNA.
DR EMBL; AJ000338; CAA04012.1; -; mRNA.
DR EMBL; AK165733; BAE38359.1; -; mRNA.
DR EMBL; CH466532; EDL11894.1; -; Genomic_DNA.
DR EMBL; BC125281; AAI25282.1; -; mRNA.
DR EMBL; BC125283; AAI25284.1; -; mRNA.
DR EMBL; BC144768; AAI44769.1; -; mRNA.
DR CCDS; CCDS17926.1; -.
DR RefSeq; NP_034843.2; NM_010713.2.
DR AlphaFoldDB; O35652; -.
DR SMR; O35652; -.
DR BioGRID; 201160; 2.
DR STRING; 10090.ENSMUSP00000136047; -.
DR PhosphoSitePlus; O35652; -.
DR PaxDb; O35652; -.
DR PRIDE; O35652; -.
DR Antibodypedia; 33466; 211 antibodies from 27 providers.
DR DNASU; 16875; -.
DR Ensembl; ENSMUST00000177846; ENSMUSP00000136047; ENSMUSG00000096225.
DR GeneID; 16875; -.
DR KEGG; mmu:16875; -.
DR UCSC; uc008rum.1; mouse.
DR CTD; 431707; -.
DR MGI; MGI:1096343; Lhx8.
DR VEuPathDB; HostDB:ENSMUSG00000096225; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000156200; -.
DR HOGENOM; CLU_027802_1_0_1; -.
DR InParanoid; O35652; -.
DR OrthoDB; 1161127at2759; -.
DR PhylomeDB; O35652; -.
DR TreeFam; TF315442; -.
DR BioGRID-ORCS; 16875; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Lhx8; mouse.
DR PRO; PR:O35652; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; O35652; protein.
DR Bgee; ENSMUSG00000096225; Expressed in primary oocyte and 104 other tissues.
DR ExpressionAtlas; O35652; baseline and differential.
DR Genevisible; O35652; MM.
DR GO; GO:0001674; C:female germ cell nucleus; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0008585; P:female gonad development; IMP:MGI.
DR GO; GO:0021884; P:forebrain neuron development; IGI:MGI.
DR GO; GO:0021879; P:forebrain neuron differentiation; IMP:MGI.
DR GO; GO:0007611; P:learning or memory; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 2: Evidence at transcript level;
KW DNA-binding; Homeobox; LIM domain; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..367
FT /note="LIM/homeobox protein Lhx8"
FT /id="PRO_0000075797"
FT DOMAIN 96..148
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 157..210
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 246..305
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 44..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 18
FT /note="V -> M (in Ref. 3; CAA04012)"
FT /evidence="ECO:0000305"
FT CONFLICT 27
FT /note="K -> M (in Ref. 3; CAA04012)"
FT /evidence="ECO:0000305"
FT CONFLICT 42..43
FT /note="LA -> G (in Ref. 3; CAA04012)"
FT /evidence="ECO:0000305"
FT CONFLICT 51
FT /note="A -> S (in Ref. 3; CAA04012)"
FT /evidence="ECO:0000305"
FT CONFLICT 72
FT /note="A -> G (in Ref. 1; BAA21649 and 3; CAA04012)"
FT /evidence="ECO:0000305"
FT CONFLICT 87..88
FT /note="SV -> PM (in Ref. 3; CAA04012)"
FT /evidence="ECO:0000305"
FT CONFLICT 327
FT /note="M -> I (in Ref. 3; CAA04012)"
FT /evidence="ECO:0000305"
FT CONFLICT 337
FT /note="V -> D (in Ref. 3; CAA04012)"
FT /evidence="ECO:0000305"
FT CONFLICT 341..342
FT /note="GT -> DG (in Ref. 3; CAA04012)"
FT /evidence="ECO:0000305"
FT CONFLICT 347
FT /note="Missing (in Ref. 3; CAA04012)"
FT /evidence="ECO:0000305"
FT CONFLICT 351
FT /note="M -> L (in Ref. 3; CAA04012)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 367 AA; 40710 MW; 1F8E3756D14DB43E CRC64;
MYWKSDQMFV CKLEGKEVPE LAVPREKCPG LMSEECGRPA ALAAGRTRKG AGEEGLVNPE
GAGDEDSCSS SAPLSPSSSP QSMASGSVCP PGKCVCSSCG LEIVDKYLLK VNDLCWHVRC
LSCSVCRTSL GRHTSCYIKD KDIFCKLDYF RRYGTRCSRC GRHIHSTDWV RRAKGNVYHL
ACFACFSCKR QLSTGEEFAL VEEKVLCRVH FDCMLDNLKR EVENGNGISV EGALLTEQDV
NHPKPAKRAR TSFTADQLQV MQAQFAQDNN PDAQTLQKLA ERTGLSRRVI QVWFQNCRAR
HKKHVSPNHS SSAPVTAVPS SRLSPPMLEE MAYSAYVPQD GTMLTALHSY MDAHQQLLDS
SPCYPIQ
