LI15A_CAEEL
ID LI15A_CAEEL Reviewed; 719 AA.
AC Q27365; Q21448;
DT 14-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Protein lin-15A;
DE AltName: Full=Abnormal cell lineage protein 15A;
GN Name=lin-15A {ECO:0000312|WormBase:ZK678.1};
GN ORFNames=ZK678.1 {ECO:0000312|WormBase:ZK678.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAA20090.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAA20090.1};
RX PubMed=7982579; DOI=10.1093/genetics/137.4.987;
RA Clark S.G., Lu X., Horvitz H.R.;
RT "The Caenorhabditis elegans locus lin-15, a negative regulator of a
RT tyrosine kinase signaling pathway, encodes two different proteins.";
RL Genetics 137:987-997(1994).
RN [2] {ECO:0000305, ECO:0000312|EMBL:CAA82855.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:CAA82855.1};
RX PubMed=8054684; DOI=10.1091/mbc.5.4.395;
RA Huang L.S., Tzou P., Sternberg P.W.;
RT "The lin-15 locus encodes two negative regulators of Caenorhabditis elegans
RT vulval development.";
RL Mol. Biol. Cell 5:395-411(1994).
RN [3] {ECO:0000312|EMBL:CAB01904.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11463372; DOI=10.1016/s1097-2765(01)00194-0;
RA Ceol C.J., Horvitz H.R.;
RT "dpl-1 DP and efl-1 E2F act with lin-35 Rb to antagonize Ras signaling in
RT C.elegans vulval development.";
RL Mol. Cell 7:461-473(2001).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16624904; DOI=10.1534/genetics.106.056465;
RA Ceol C.J., Stegmeier F., Harrison M.M., Horvitz H.R.;
RT "Identification and classification of genes that act antagonistically to
RT let-60 Ras signaling in Caenorhabditis elegans vulval development.";
RL Genetics 173:709-726(2006).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=21196525; DOI=10.1534/genetics.110.124487;
RA Davison E.M., Saffer A.M., Huang L.S., DeModena J., Sternberg P.W.,
RA Horvitz H.R.;
RT "The LIN-15A and LIN-56 transcriptional regulators interact to negatively
RT regulate EGF/Ras signaling in Caenorhabditis elegans vulval cell-fate
RT determination.";
RL Genetics 187:803-815(2011).
CC -!- FUNCTION: Synthetic multivulva (synMuv) class A protein
CC (PubMed:7982579, PubMed:8054684). SynMuv proteins are required to
CC repress the induction of vulval development (PubMed:7982579,
CC PubMed:8054684). Acts redundantly with SynMuv class B protein lin-15B,
CC and lin-35 to negatively regulate vulval development, most likely
CC through antagonization of the Ras-signaling pathway (PubMed:8054684,
CC PubMed:16624904). May also negatively regulate vulval development in
CC association with other SynMuv class B proteins such as dpl-1 and efl-1
CC (PubMed:11463372). Regulates let-23 basal activity (PubMed:8054684).
CC Required for the correct expression and/or stability of lin-56
CC (PubMed:21196525). {ECO:0000269|PubMed:16624904,
CC ECO:0000269|PubMed:21196525, ECO:0000269|PubMed:7982579,
CC ECO:0000269|PubMed:8054684}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21196525}.
CC -!- DISRUPTION PHENOTYPE: Greatly reduced nuclear accumulation of lin-56
CC protein and increased mRNA levels (PubMed:21196525). No obvious vulval
CC development defects (PubMed:8054684, PubMed:11463372). Double knockout
CC with the synthetic multivulva class B protein lin-15B, dpl-1, efl-1 or
CC lin-35 results in a multiple vulva (Muv) phenotype (PubMed:8054684,
CC PubMed:11463372, PubMed:16624904). {ECO:0000269|PubMed:11463372,
CC ECO:0000269|PubMed:16624904, ECO:0000269|PubMed:21196525,
CC ECO:0000269|PubMed:8054684}.
CC -!- MISCELLANEOUS: The proteins lin-15A and lin-15B are produced from a
CC single precursor mRNA with non-overlapping transcripts where lin-15B is
CC the upstream transcript and lin-15A is the downstream one. The
CC precursor mRNA is cleaved at the polyadenylation site and a 22-
CC nucleotide SL2 leader sequence is trans-spliced to the 5'-end of lin-
CC 15A to allow its translation.
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DR EMBL; U10411; AAA20087.1; -; mRNA.
DR EMBL; U10413; AAA20090.1; -; Genomic_DNA.
DR EMBL; Z29967; CAA82855.1; -; Genomic_DNA.
DR EMBL; Z79605; CAB01904.1; -; Genomic_DNA.
DR PIR; T27977; T27977.
DR RefSeq; NP_510588.1; NM_078187.5.
DR AlphaFoldDB; Q27365; -.
DR BioGRID; 46555; 31.
DR IntAct; Q27365; 27.
DR MINT; Q27365; -.
DR STRING; 6239.ZK678.1; -.
DR iPTMnet; Q27365; -.
DR EPD; Q27365; -.
DR PaxDb; Q27365; -.
DR EnsemblMetazoa; ZK678.1.1; ZK678.1.1; WBGene00023498.
DR GeneID; 181663; -.
DR KEGG; cel:CELE_ZK678.1; -.
DR UCSC; ZK678.1; c. elegans.
DR CTD; 181663; -.
DR WormBase; ZK678.1; CE15383; WBGene00023498; lin-15A.
DR eggNOG; ENOG502TIKD; Eukaryota.
DR HOGENOM; CLU_384620_0_0_1; -.
DR OrthoDB; 1822361at2759; -.
DR SignaLink; Q27365; -.
DR PRO; PR:Q27365; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00023498; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0040027; P:negative regulation of vulval development; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0050821; P:protein stabilization; IMP:UniProtKB.
DR GO; GO:0042659; P:regulation of cell fate specification; IMP:WormBase.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR GO; GO:0040025; P:vulval development; IMP:UniProtKB.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Nucleus; Reference proteome;
KW Sexual differentiation.
FT CHAIN 1..719
FT /note="Protein lin-15A"
FT /id="PRO_0000260043"
FT REGION 179..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 419..464
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 559..626
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 684..719
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 440..454
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 559..585
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 607..622
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 697..712
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 685..719
FT /note="KQVAAAPSEPKHIPPTHMEKKPEELLMDPKPEPIF -> NKWPPHRVSQNIF
FT RQRTWKRSRKSCSWIQSLSQFFKRS (in Ref. 2; CAA82855)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 719 AA; 80062 MW; 8E69CF00D419DD7F CRC64;
MLAPAAPAKD VVSADEKEEI IAKRKFRMKN VDAMRMSSLA NDRMAFNKKC NALAMKFVKS
AGIGTDALQL TCFQELVRHF NPIAAVVVGV KREPNSNVQA EKKTIPKVKT IQTPTQSMES
VRLLQEKKAS ATEEQSAESA SIMKHFANTI PNSTPTQSVK DVLTAAASKG QFKSSAEIFS
HFPSEPSPSK PRATREGSQP SDYTYCTYLT PCILCEKALL MRESIAMTDN EAVKVLMAAV
MSGHFRMATA EKAIRHERLR MCYDHVDFVY EMMCDAFEAK TESEINEMPP DRLMRGHDIY
RALKRVGDLH KGKVTSNTPL YSFKNSIKSY YRNHVPRMVN GSLSKPSPKP FSELVALLQS
VPPSTNLNEL LNHNLSLSDA DKQELIQLIN GKDNRFTSRR RKIEDILDNK FAAAAAKAYR
DHSEDAPSEP YIPNQSEMQN TVERRKRKLH SPEQDDAGSS SISWNAKKTK TPIDYVHLAT
RVLEGHSIAD EALLHKSKVS YARNAFGEKP SSPTPPSAPL KFCVVNGKKY LRFENGTGPP
KVVVQGNVVL RTNTLKDALT TAPRAQNQPS TSTDSSSSSE MEGIRQSFGA PQKEEEEEEL
VPTLLQNKPT HVESSSPVEK KPPTKTNVEK PAVRLGRMLT TAFGSMSYRT RKSVENKTDL
LNQPTSASPR RMIKVVRNRN PHLAKQVAAA PSEPKHIPPT HMEKKPEELL MDPKPEPIF
