LI15B_CAEEL
ID LI15B_CAEEL Reviewed; 1440 AA.
AC Q27395; Q94409; Q94414;
DT 14-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Protein lin-15B;
DE AltName: Full=Abnormal cell lineage protein 15B;
GN Name=lin-15B {ECO:0000312|WormBase:ZK662.4};
GN ORFNames=ZK662.4 {ECO:0000312|WormBase:ZK662.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAA20089.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAA20089.1};
RX PubMed=7982579; DOI=10.1093/genetics/137.4.987;
RA Clark S.G., Lu X., Horvitz H.R.;
RT "The Caenorhabditis elegans locus lin-15, a negative regulator of a
RT tyrosine kinase signaling pathway, encodes two different proteins.";
RL Genetics 137:987-997(1994).
RN [2] {ECO:0000305, ECO:0000312|EMBL:CAA82854.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:CAA82854.1};
RX PubMed=8054684; DOI=10.1091/mbc.5.4.395;
RA Huang L.S., Tzou P., Sternberg P.W.;
RT "The lin-15 locus encodes two negative regulators of Caenorhabditis elegans
RT vulval development.";
RL Mol. Biol. Cell 5:395-411(1994).
RN [3] {ECO:0000312|EMBL:CAB01901.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11463372; DOI=10.1016/s1097-2765(01)00194-0;
RA Ceol C.J., Horvitz H.R.;
RT "dpl-1 DP and efl-1 E2F act with lin-35 Rb to antagonize Ras signaling in
RT C.elegans vulval development.";
RL Mol. Cell 7:461-473(2001).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=17237514; DOI=10.1534/genetics.106.068148;
RA Reddien P.W., Andersen E.C., Huang M.C., Horvitz H.R.;
RT "DPL-1 DP, LIN-35 Rb and EFL-1 E2F act with the MCD-1 zinc-finger protein
RT to promote programmed cell death in Caenorhabditis elegans.";
RL Genetics 175:1719-1733(2007).
CC -!- FUNCTION: Synthetic multivulva (synMuv) class B protein
CC (PubMed:7982579, PubMed:8054684). SynMuv proteins are required to
CC repress the induction of vulval development (PubMed:7982579,
CC PubMed:8054684, PubMed:11463372). Acts redundantly with SynMuv class A
CC protein lin-15A to negatively regulate vulval development
CC (PubMed:8054684). Regulates let-23 basal activity (PubMed:8054684).
CC {ECO:0000269|PubMed:11463372, ECO:0000269|PubMed:7982579,
CC ECO:0000269|PubMed:8054684}.
CC -!- DISRUPTION PHENOTYPE: No obvious vulval development defects
CC (PubMed:8054684, PubMed:11463372). Double knockout with the synthetic
CC multivulva class A protein lin-15A results in a multiple vulva (Muv)
CC phenotype (PubMed:8054684). Double knockout with the programmed cell
CC death regulator mcd-1 results in 100% lethality during the L1 stage of
CC larval development (PubMed:17237514). {ECO:0000269|PubMed:11463372,
CC ECO:0000269|PubMed:17237514, ECO:0000269|PubMed:8054684}.
CC -!- MISCELLANEOUS: The proteins lin-15A and lin-15B are produced from a
CC single precursor mRNA with non-overlapping transcripts where lin-15B is
CC the upstream transcript and lin-15A is the downstream one. The
CC precursor mRNA is cleaved at the polyadenylation site and a 22-
CC nucleotide SL2 leader sequence is trans-spliced to the 5'-end of lin-
CC 15A to allow its translation.
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DR EMBL; U10412; AAA20088.1; -; mRNA.
DR EMBL; U10413; AAA20089.1; -; Genomic_DNA.
DR EMBL; Z29967; CAA82854.1; -; Genomic_DNA.
DR EMBL; Z79604; CAB01901.1; -; Genomic_DNA.
DR EMBL; Z79605; CAB01901.1; JOINED; Genomic_DNA.
DR PIR; T27942; T27942.
DR RefSeq; NP_510587.1; NM_078186.6.
DR AlphaFoldDB; Q27395; -.
DR BioGRID; 46554; 15.
DR STRING; 6239.ZK662.4.1; -.
DR iPTMnet; Q27395; -.
DR EPD; Q27395; -.
DR PaxDb; Q27395; -.
DR PeptideAtlas; Q27395; -.
DR PRIDE; Q27395; -.
DR EnsemblMetazoa; ZK662.4.1; ZK662.4.1; WBGene00023497.
DR EnsemblMetazoa; ZK662.4.2; ZK662.4.2; WBGene00023497.
DR GeneID; 181662; -.
DR KEGG; cel:CELE_ZK662.4; -.
DR UCSC; ZK662.4.2; c. elegans.
DR CTD; 181662; -.
DR WormBase; ZK662.4; CE15381; WBGene00023497; lin-15B.
DR eggNOG; KOG1121; Eukaryota.
DR GeneTree; ENSGT00390000009480; -.
DR HOGENOM; CLU_004462_0_0_1; -.
DR InParanoid; Q27395; -.
DR OMA; VCCYCSK; -.
DR OrthoDB; 223749at2759; -.
DR PRO; PR:Q27395; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00023497; Expressed in embryo and 4 other tissues.
DR GO; GO:0005634; C:nucleus; NAS:WormBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0046580; P:negative regulation of Ras protein signal transduction; IMP:UniProtKB.
DR GO; GO:0040027; P:negative regulation of vulval development; IMP:UniProtKB.
DR GO; GO:0042659; P:regulation of cell fate specification; IMP:WormBase.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR InterPro; IPR040129; Lin-15B-like.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR006612; THAP_Znf.
DR PANTHER; PTHR22716; PTHR22716; 1.
DR SMART; SM00692; DM3; 1.
DR SMART; SM00980; THAP; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR PROSITE; PS50950; ZF_THAP; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Metal-binding;
KW Reference proteome; Repeat; Sexual differentiation; Zinc; Zinc-finger.
FT CHAIN 1..1440
FT /note="Protein lin-15B"
FT /id="PRO_0000260044"
FT ZN_FING 1135..1209
FT /note="THAP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00309"
FT DNA_BIND 1317..1329
FT /note="A.T hook 1"
FT /evidence="ECO:0000255"
FT DNA_BIND 1418..1430
FT /note="A.T hook 2"
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 48..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 618..660
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 738..769
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1239..1281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1298..1350
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1395..1440
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 626..641
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 752..769
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1252..1267
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1440 AA; 163594 MW; F5B649C642CA07E0 CRC64;
MQTLKTARLT SNPASIPTSS SSSAISAAAI QKTLDAVNRP PAVRASGILR HRTLPAPTQE
TAHHLDADPK TTELMARFFI SQGIPFECAH EPAFLELMKH VDPNCVIPPT NVTKKLVDKI
STSSKPQVNY TKTVGPLSVT IDICGDEDEK YLAFSIHYFE DLYERKNAIY LRKLLLTELD
SNSLLTNIRR SVNSYSFSNV KFTNIVCPNE EICKLVEESA VVKRYNVCFY NYVTRFVADL
MEIEEFSSGL TQLRTFVRYM KQNSDMYSKF RRMQLQKNAE LDIPSIDSGD WHSTAIFLTR
CLVWHDTFTE FCGKLDILHY IDNETFNHLI YLQRLLQQCM KHCRELSIPN NSISQVVPAI
MSIRNFIASN SMGYRFQKRI RDSFTTSFKE ITSGPSQDRY DIATLLDPRF AYRDTVYTAQ
TWRSLEKKVI DDFVNSDLQN DKNFYQDISI LNQEQRYDII KKEFAYYRQT SFVERPEENE
NSNHWWGMRQ TDMEFLAVIA REYLASPAVS IDAGYYFGNG GKFQHICHTY SHQRLENCLA
LAGNYQTFRG KGASVDVISQ SMIETLNNTA SRLQKQVHLG LYAHGVDNIS SDRDVQSIVG
HHYPPMPTVA NYDIPHVPKE EEKPPVANLQ STSSPATSSP TIIRPRAAPP PRTLAQGRPI
PLNGKELKAV PIRQIPLQVR PLPPRPANVP IVPRPTVPQQ FIKAPAPKPI TLQAVVCSIP
EKEIKKETED VALLEKIKDE PLDEDDFNHP STDPVPNRTT ASSQGPSSYP RKIVVLASKL
PTSQSSSPST ATSAQARSHV TTAQLIRCGP SEGTVPQKIH SHNFVQKFAQ KQNFVHKYAL
NSQDHTGRLN QTVPMRAALR LPNSEQKSGA PSSINGKVQR DDFKLEPLDD FNGEPDYDNL
IGAQRLMYSD NLNDASAEDA FARHRVTMEF QKRRACNRRC AVCGHLEIHE RLKNVTIENE
KLLIMLGCIY RGEFTLGQAQ LFMARESKTY ICRLHFLETL DEIYQMLRLK SADDILICPL
DLIQNALITV SALRPHIIAS QLRKILHDFA ERNNHLRETP AELKKLGQQY FDYREPEPEP
ERNDVDEQEI IPKLFRQPRK QVLEADQHDG TVKVIEQEDF KLPTVKPSEN EECDNPGVCC
FCSKRGDRGG MLRVPRSEER LARWVDKLGP EFEARLHTNT ENLICRSHFP DAAFSSRGRL
LKGMIPDAAP EKVETTYIIQ GNNFLKLKER KSGTDKNSAI DLANMLNPDG VEYTQEEEEE
EEYEEMSRSP TEETSDDEPS QAAVYNNAPV IKRTYRKREL SNEDGPLNLV TPPAHTPNPR
GRPRKYPKNS VTPEAEKSLT DYDYNPGTSQ RRALKKGYVQ LEDGEIVGED CEYVPEKTPS
GRLIRQAVAR RSFAFADEEE EEEEYEESPI VKKPKIAGRP VGRPRKDANK LPTPTPPSNE
