LIAF_BACSU
ID LIAF_BACSU Reviewed; 241 AA.
AC O32199; Q7B2J5;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Protein LiaF;
GN Name=liaF; Synonyms=yvqF; OrderedLocusNames=BSU33100;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9639930; DOI=10.1099/00221287-144-6-1593;
RA Wipat A., Brignell C.S., Guy J.B., Rose M., Emmerson P.T., Harwood C.R.;
RT "The yvsA-yvqA (293 degrees - 289 degrees) region of the Bacillus subtilis
RT chromosome containing genes involved in metal ion uptake and a putative
RT sigma factor.";
RL Microbiology 144:1593-1600(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP INDUCTION BY VANCOMYCIN.
RC STRAIN=168 / CU1065;
RX PubMed=12207695; DOI=10.1046/j.1365-2958.2002.03050.x;
RA Cao M., Wang T., Ye R., Helmann J.D.;
RT "Antibiotics that inhibit cell wall biosynthesis induce expression of the
RT Bacillus subtilis sigma(W) and sigma(M) regulons.";
RL Mol. Microbiol. 45:1267-1276(2002).
RN [4]
RP INDUCTION BY BACITRACIN.
RC STRAIN=168 / CU1065;
RX PubMed=14651641; DOI=10.1046/j.1365-2958.2003.03786.x;
RA Mascher T., Margulis N.G., Wang T., Ye R.W., Helmann J.D.;
RT "Cell wall stress responses in Bacillus subtilis: the regulatory network of
RT the bacitracin stimulon.";
RL Mol. Microbiol. 50:1591-1604(2003).
RN [5]
RP INDUCTION BY ANTIBIOTICS.
RX PubMed=15273097; DOI=10.1128/aac.48.8.2888-2896.2004;
RA Mascher T., Zimmer S.L., Smith T.-A., Helmann J.D.;
RT "Antibiotic-inducible promoter regulated by the cell envelope stress-
RT sensing two-component system LiaRS of Bacillus subtilis.";
RL Antimicrob. Agents Chemother. 48:2888-2896(2004).
RN [6]
RP INDUCTION BY STRESS.
RX PubMed=15856219; DOI=10.1007/s00253-005-1898-1;
RA Hyyrylaeinen H.-L., Sarvas M., Kontinen V.P.;
RT "Transcriptome analysis of the secretion stress response of Bacillus
RT subtilis.";
RL Appl. Microbiol. Biotechnol. 67:389-396(2005).
RN [7]
RP INDUCTION BY LL-37; PG-1 AND TRITON X-100.
RC STRAIN=168;
RX PubMed=15870467; DOI=10.1099/mic.0.27761-0;
RA Pietiaeinen M., Gardemeister M., Mecklin M., Leskelae S., Sarvas M.,
RA Kontinen V.P.;
RT "Cationic antimicrobial peptides elicit a complex stress response in
RT Bacillus subtilis that involves ECF-type sigma factors and two-component
RT signal transduction systems.";
RL Microbiology 151:1577-1592(2005).
RN [8]
RP DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=17921301; DOI=10.1128/jb.01181-07;
RA Butcher B.G., Lin Y.-P., Helmann J.D.;
RT "The yydFGHIJ operon of Bacillus subtilis encodes a peptide that induces
RT the LiaRS two-component system.";
RL J. Bacteriol. 189:8616-8625(2007).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Induced, via the two-component regulatory system LiaS/LiaR,
CC by antibiotics (vancomycin, bacitracin, nisin and ramoplanin), cationic
CC antimicrobial peptides (human LL-37 and porcine PG-1), Triton X-100 and
CC severe secretion stress. {ECO:0000269|PubMed:12207695,
CC ECO:0000269|PubMed:14651641, ECO:0000269|PubMed:15273097,
CC ECO:0000269|PubMed:15856219, ECO:0000269|PubMed:15870467}.
CC -!- DISRUPTION PHENOTYPE: Up-regulates expression of liaI which in turn
CC induces the liaRS two-component regulatory system.
CC {ECO:0000269|PubMed:17921301}.
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DR EMBL; AJ223978; CAA11743.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15300.1; -; Genomic_DNA.
DR PIR; G70045; G70045.
DR RefSeq; NP_391190.1; NC_000964.3.
DR RefSeq; WP_003242605.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; O32199; -.
DR SMR; O32199; -.
DR STRING; 224308.BSU33100; -.
DR PaxDb; O32199; -.
DR PRIDE; O32199; -.
DR EnsemblBacteria; CAB15300; CAB15300; BSU_33100.
DR GeneID; 938469; -.
DR KEGG; bsu:BSU33100; -.
DR PATRIC; fig|224308.179.peg.3588; -.
DR eggNOG; COG4758; Bacteria.
DR OMA; HQWIGAA; -.
DR PhylomeDB; O32199; -.
DR BioCyc; BSUB:BSU33100-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR016975; Cell_wall-act_antibiotic_resp.
DR InterPro; IPR024425; DUF2154.
DR Pfam; PF09922; DUF2154; 1.
DR PIRSF; PIRSF031509; Cell_wall_LiaF/YvqF; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..241
FT /note="Protein LiaF"
FT /id="PRO_0000084414"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 241 AA; 27025 MW; C7B714AF84312F96 CRC64;
MTKKQLLGLI IALFGISMFL QIIGIGDLLF WPLFFLIAGY FLKKYSRDWL GSVMYIFAAF
LFLKNLFSIT FNLFGYAFAA FLIYAGYRLI KGKPIFEPNE KQVNLNKKEH HEPPKDVKHP
DMRSFFIGEL QMMKQPFDLN DLNVSGFIGD IKIDLSKAMI PEGESTIVIS GVIGNVDIYV
PSDLEVAVSS AVFIGDINLI GSKKSGLSTK VYAASTDFSE SKRRVKVSVS LFIGDVDVKY
V
