LIAG_BACSU
ID LIAG_BACSU Reviewed; 290 AA.
AC O32200; Q7B2J6;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 14-APR-2009, sequence version 2.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Protein LiaG;
DE Flags: Precursor;
GN Name=liaG; Synonyms=yvqG; OrderedLocusNames=BSU33110;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9639930; DOI=10.1099/00221287-144-6-1593;
RA Wipat A., Brignell C.S., Guy J.B., Rose M., Emmerson P.T., Harwood C.R.;
RT "The yvsA-yvqA (293 degrees - 289 degrees) region of the Bacillus subtilis
RT chromosome containing genes involved in metal ion uptake and a putative
RT sigma factor.";
RL Microbiology 144:1593-1600(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO N-TERMINUS.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP INDUCTION BY VANCOMYCIN.
RC STRAIN=168 / CU1065;
RX PubMed=12207695; DOI=10.1046/j.1365-2958.2002.03050.x;
RA Cao M., Wang T., Ye R., Helmann J.D.;
RT "Antibiotics that inhibit cell wall biosynthesis induce expression of the
RT Bacillus subtilis sigma(W) and sigma(M) regulons.";
RL Mol. Microbiol. 45:1267-1276(2002).
RN [5]
RP INDUCTION BY BACITRACIN.
RC STRAIN=168 / CU1065;
RX PubMed=14651641; DOI=10.1046/j.1365-2958.2003.03786.x;
RA Mascher T., Margulis N.G., Wang T., Ye R.W., Helmann J.D.;
RT "Cell wall stress responses in Bacillus subtilis: the regulatory network of
RT the bacitracin stimulon.";
RL Mol. Microbiol. 50:1591-1604(2003).
RN [6]
RP INDUCTION BY ANTIBIOTICS.
RX PubMed=15273097; DOI=10.1128/aac.48.8.2888-2896.2004;
RA Mascher T., Zimmer S.L., Smith T.-A., Helmann J.D.;
RT "Antibiotic-inducible promoter regulated by the cell envelope stress-
RT sensing two-component system LiaRS of Bacillus subtilis.";
RL Antimicrob. Agents Chemother. 48:2888-2896(2004).
RN [7]
RP INDUCTION BY STRESS.
RX PubMed=15856219; DOI=10.1007/s00253-005-1898-1;
RA Hyyrylaeinen H.-L., Sarvas M., Kontinen V.P.;
RT "Transcriptome analysis of the secretion stress response of Bacillus
RT subtilis.";
RL Appl. Microbiol. Biotechnol. 67:389-396(2005).
RN [8]
RP INDUCTION BY LL-37; PG-1 AND TRITON X-100.
RC STRAIN=168;
RX PubMed=15870467; DOI=10.1099/mic.0.27761-0;
RA Pietiaeinen M., Gardemeister M., Mecklin M., Leskelae S., Sarvas M.,
RA Kontinen V.P.;
RT "Cationic antimicrobial peptides elicit a complex stress response in
RT Bacillus subtilis that involves ECF-type sigma factors and two-component
RT signal transduction systems.";
RL Microbiology 151:1577-1592(2005).
RN [9]
RP DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=17921301; DOI=10.1128/jb.01181-07;
RA Butcher B.G., Lin Y.-P., Helmann J.D.;
RT "The yydFGHIJ operon of Bacillus subtilis encodes a peptide that induces
RT the LiaRS two-component system.";
RL J. Bacteriol. 189:8616-8625(2007).
CC -!- INDUCTION: Induced, via the two-component regulatory system LiaS/LiaR,
CC by antibiotics (vancomycin, bacitracin, nisin and ramoplanin), cationic
CC antimicrobial peptides (human LL-37 and porcine PG-1), Triton X-100 and
CC severe secretion stress. {ECO:0000269|PubMed:12207695,
CC ECO:0000269|PubMed:14651641, ECO:0000269|PubMed:15273097,
CC ECO:0000269|PubMed:15856219, ECO:0000269|PubMed:15870467}.
CC -!- DISRUPTION PHENOTYPE: Up-regulates expression of liaI which in turn
CC induces the liaRS two-component regulatory system.
CC {ECO:0000269|PubMed:17921301}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA11742.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AJ223978; CAA11742.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AL009126; CAB15301.2; -; Genomic_DNA.
DR PIR; H70045; H70045.
DR RefSeq; NP_391191.2; NC_000964.3.
DR RefSeq; WP_003244420.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; O32200; -.
DR SMR; O32200; -.
DR STRING; 224308.BSU33110; -.
DR PaxDb; O32200; -.
DR PRIDE; O32200; -.
DR EnsemblBacteria; CAB15301; CAB15301; BSU_33110.
DR GeneID; 938471; -.
DR KEGG; bsu:BSU33110; -.
DR PATRIC; fig|224308.179.peg.3589; -.
DR eggNOG; COG3595; Bacteria.
DR OMA; KEQHHIT; -.
DR BioCyc; BSUB:BSU33110-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR InterPro; IPR025164; DUF4097.
DR Pfam; PF13349; DUF4097; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Signal; Stress response.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..290
FT /note="Protein LiaG"
FT /id="PRO_0000084415"
FT REGION 257..290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 290 AA; 30262 MW; 214584684EF570F7 CRC64;
MKKMLGKLLI TAGILVFFAV VVKDVFAAGL GFINGTEADS ASASPRDIDS MVIESDSKDV
RIIAEERSDI SAGISGDSGK LFVTENKRKL ELTVKEKEFQ FLNGFNRSTL IVRLPYDYKG
DLAVRTSSGD VSVAGNDHLA LSGLNAVSAS GNTSVTDVRI QDLKVKASSG DVSISNTVSK
TAGIDLASGD ANLVHVSGSL DVKMTSGDFN AVLKKVTGPV SVTLTSGDAN VSLPQNGSFA
VNALSASGDV SSPYSFADKG HKEQHHITGT QGSGRHPIDI KTDSGDLAIR
