LIAR_BACSU
ID LIAR_BACSU Reviewed; 211 AA.
AC O32197; Q7B2J3;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Transcriptional regulatory protein LiaR;
GN Name=liaR; Synonyms=yvqC; OrderedLocusNames=BSU33080;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9639930; DOI=10.1099/00221287-144-6-1593;
RA Wipat A., Brignell C.S., Guy J.B., Rose M., Emmerson P.T., Harwood C.R.;
RT "The yvsA-yvqA (293 degrees - 289 degrees) region of the Bacillus subtilis
RT chromosome containing genes involved in metal ion uptake and a putative
RT sigma factor.";
RL Microbiology 144:1593-1600(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION.
RX PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA Fujita Y.;
RT "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT regulatory systems.";
RL J. Bacteriol. 183:7365-7370(2001).
RN [4]
RP INDUCTION BY BACITRACIN.
RC STRAIN=168 / CU1065;
RX PubMed=14651641; DOI=10.1046/j.1365-2958.2003.03786.x;
RA Mascher T., Margulis N.G., Wang T., Ye R.W., Helmann J.D.;
RT "Cell wall stress responses in Bacillus subtilis: the regulatory network of
RT the bacitracin stimulon.";
RL Mol. Microbiol. 50:1591-1604(2003).
RN [5]
RP FUNCTION, AND INDUCTION BY ANTIBIOTICS.
RX PubMed=15273097; DOI=10.1128/aac.48.8.2888-2896.2004;
RA Mascher T., Zimmer S.L., Smith T.-A., Helmann J.D.;
RT "Antibiotic-inducible promoter regulated by the cell envelope stress-
RT sensing two-component system LiaRS of Bacillus subtilis.";
RL Antimicrob. Agents Chemother. 48:2888-2896(2004).
RN [6]
RP INDUCTION BY STRESS.
RX PubMed=15856219; DOI=10.1007/s00253-005-1898-1;
RA Hyyrylaeinen H.-L., Sarvas M., Kontinen V.P.;
RT "Transcriptome analysis of the secretion stress response of Bacillus
RT subtilis.";
RL Appl. Microbiol. Biotechnol. 67:389-396(2005).
RN [7]
RP INDUCTION BY LL-37; PG-1 AND TRITON X-100.
RC STRAIN=168;
RX PubMed=15870467; DOI=10.1099/mic.0.27761-0;
RA Pietiaeinen M., Gardemeister M., Mecklin M., Leskelae S., Sarvas M.,
RA Kontinen V.P.;
RT "Cationic antimicrobial peptides elicit a complex stress response in
RT Bacillus subtilis that involves ECF-type sigma factors and two-component
RT signal transduction systems.";
RL Microbiology 151:1577-1592(2005).
CC -!- FUNCTION: Member of the two-component regulatory system LiaS/LiaR
CC probably involved in response to a subset of cell wall-active
CC antibiotics that interfere with the lipid II cycle in the cytoplasmic
CC membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also
CC involved in response to cationic antimicrobial peptides and secretion
CC stress. LiaR regulates the transcription of the liaIHGFSR operon.
CC {ECO:0000269|PubMed:11717295, ECO:0000269|PubMed:15273097}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Autoregulated. Induced by antibiotics (vancomycin,
CC bacitracin, nisin and ramoplanin), cationic antimicrobial peptides
CC (human LL-37 and porcine PG-1), Triton X-100 and severe secretion
CC stress. {ECO:0000269|PubMed:14651641, ECO:0000269|PubMed:15273097,
CC ECO:0000269|PubMed:15856219, ECO:0000269|PubMed:15870467}.
CC -!- PTM: Phosphorylated by LiaS. {ECO:0000305}.
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DR EMBL; AJ223978; CAA11745.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15298.1; -; Genomic_DNA.
DR PIR; E70045; E70045.
DR RefSeq; NP_391188.1; NC_000964.3.
DR RefSeq; WP_003243201.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; O32197; -.
DR SMR; O32197; -.
DR STRING; 224308.BSU33080; -.
DR PaxDb; O32197; -.
DR PRIDE; O32197; -.
DR EnsemblBacteria; CAB15298; CAB15298; BSU_33080.
DR GeneID; 935957; -.
DR KEGG; bsu:BSU33080; -.
DR PATRIC; fig|224308.179.peg.3586; -.
DR eggNOG; COG2197; Bacteria.
DR InParanoid; O32197; -.
DR OMA; NVLRKTQ; -.
DR PhylomeDB; O32197; -.
DR BioCyc; BSUB:BSU33080-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR Pfam; PF00196; GerE; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS00622; HTH_LUXR_1; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 2: Evidence at transcript level;
KW Activator; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation; Two-component regulatory system.
FT CHAIN 1..211
FT /note="Transcriptional regulatory protein LiaR"
FT /id="PRO_0000081114"
FT DOMAIN 3..119
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 143..208
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 167..186
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT MOD_RES 54
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 211 AA; 23125 MW; ADBA4BAE61E3F5A8 CRC64;
MIRVLLIDDH EMVRMGLAAF LEAQPDIEVI GEASDGSEGV RLAVELSPDV ILMDLVMEGM
DGIEATKQIC RELSDPKIIV LTSFIDDDKV YPVIEAGALS YLLKTSKAAE IADAIRAASK
GEPKLESKVA GKVLSRLRHS GENALPHESL TKRELEILCL IAEGKTNKEI GEELFITIKT
VKTHITNILS KLDVSDRTQA AVYAHRNHLV N
